当前位置： SCI文献检索 > SPINE期刊下所有文献 > Rheological and dynamic integrity of simulated degenerated disc and consequences after cross-linker augmentation.

Rheological and dynamic integrity of simulated degenerated disc and consequences after cross-linker augmentation.

Abstract：

STUDY DESIGN:An in situ study using whole-organ culture system. OBJECTIVE:To study the effect of disc degeneration at different stages on its rheological and dynamic properties and to investigate the efficacy of exogenous cross-linking therapy. SUMMARY OF BACKGROUND DATA:Disc degeneration can involve protein denaturation or microdefects to the disc's collagen fiber network. A disc degeneration model using whole-organ culture technique can be effectively used for the screening of treatments of degenerated discs. Exogenous cross-linking therapy has been shown to enhance the mechanical properties of the disc by cross-linking collagen. However, the efficacy of this therapy on the degenerated disc is unclear. METHODS:A total of 40 porcine thoracic discs were assigned to 5 groups: intact discs, moderately degenerated discs, moderately degenerated discs with cross-linker augmentation, severely degenerated discs, and severely degenerated discs with cross-linker augmentation. The disc degeneration was simulated by trypsin digestion and mechanical fatigue loading. Rheological properties, dynamic properties, water content, and histological analysis were conducted after a 7-day incubation. RESULTS:The mechanical properties of moderate degenerated discs significantly decrease both in rheological and dynamic properties, and laminate structure disorganization was observed. Mechanical defects of severely degenerated discs resulted in disc height loss, an increase in the aggregate modulus and stiffness modulus, and a decrease in the damping coefficient, hydraulic permeability, and water content. Cross-linker augmentation significantly recovered mechanical properties of moderately degenerated discs and restored the water content compared with the intact disc. However, the augmentation did not fully repair the severely degenerated discs. CONCLUSION:Trypsin-induced extracellular matrix damage resulted in a change of the disc's biomechanics. Cross-linker augmentation recovers the rheological and dynamic properties of moderately degenerated discs but not of the severely degenerated discs. The genipin cross-linker may be able to improve the proteoglycan depletion effect in the nucleus pulposus but may not be effective to restore the structural damage in the collagen molecule of the anulus fibrosus.

journal_name

Spine (Phila Pa 1976)

journal_title

Spine

authors

Hsu YC,Kuo YW,Chang YC,Nikkhoo M,Wang JL

doi

10.1097/BRS.0b013e3182a3d09d

subject

Has Abstract

pub_date

2013-11-01 00:00:00

pages

E1446-53

issue

eissn

0362-2436

issn

1528-1159

journal_volume

pub_type

杂志文章

在线工具

Rheological and dynamic integrity of simulated degenerated disc and consequences after cross-linker augmentation.