Abstract:
:Precise and effective genome-editing tools are essential for functional genomics and gene therapy. Targeting nucleases have been successfully used to edit genomes. However, whole-locus or element-specific deletions abolishing transcript expression have not previously been reported. Here, we show heritable targeting of locus-specific deletions in the zebrafish nodal-related genes squint (sqt) and cyclops (cyc). Our strategy of heritable chromosomal editing can be used for disease modeling, analyzing gene clusters, regulatory regions, and determining the functions of non-coding RNAs in genomes.
journal_name
Genome Bioljournal_title
Genome biologyauthors
Lim S,Wang Y,Yu X,Huang Y,Featherstone MS,Sampath Kdoi
10.1186/gb-2013-14-7-r69subject
Has Abstractpub_date
2013-07-01 00:00:00pages
R69issue
7eissn
1474-7596issn
1474-760Xpii
gb-2013-14-7-r69journal_volume
14pub_type
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