Glutamine-induced production and secretion of Helicobacter pylori gamma-glutamyltranspeptidase at low pH and its putative role in glutathione transport.

abstract：:Energy-efficient metabolic responses were often noted in high-productive cultures. To better understand these metabolic responses, an investigation into the relationship between metabolic responses and energy regulation was conducted via a comparative analysis among cultures with different energy source supplies. Both...

journal_title：Journal of microbiology and biotechnology

authors： Sun YT,Zhao L,Ye ZY,Fan L,Liu XP,Tan WS

更新日期：2013-11-28 00:00:00

abstract：:Small heat shock proteins (sHSPs) function as molecular chaperones that protect cells against environmental stresses. In the present study, the genes of hsp17.6 and hsp17.7, cytosolic class I sHSPs, were cloned from a tropical plant, Ageratina adenophorum. Their C-terminal domains were highly conserved with those of s...

journal_title：Journal of microbiology and biotechnology

authors： Gong W,Yue M,Xie B,Wan F,Guo J

更新日期：2009-12-01 00:00:00

abstract：:With the advantages of biocatalytic method, enzymes have been excavated for the synthesis of chiral amino acids by the reductive amination of ketones, offering a promising way of producing pharmaceutical intermediates. In this work, a robust phenylalanine dehydrogenase (PheDH) with wide substrate spectrum and high cat...

journal_title：Journal of microbiology and biotechnology

authors： Jiang W,Wang Y

更新日期：2020-01-28 00:00:00

abstract：:An investigation was conducted on the enhancement of production and purification of an oxidant and SDS-stable alkaline protease (BHAP) secreted by an alkalophilic Bacillus horikoshii, which was screened from the body fluid of a unique Korean polychaeta (Periserrula leucophryna) living in the tidal mud flats of Kwangwh...

journal_title：Journal of microbiology and biotechnology

authors： Joo HS,Choi JW

更新日期：2012-01-01 00:00:00

abstract：:A new versatile acrylonitrile-bioconverting strain isolated from a petroleum-contaminated sludge sample and identified as Rhodococcus ruber AKSH-84 was used for optimization of medium and biotransformation conditions for nitrilase activity to produce acrylic acid. A simple and rapid HPLC protocol was optimized for qua...

journal_title：Journal of microbiology and biotechnology

authors： Kamal A,Kumar MS,Kumar CG,Shaik T

更新日期：2011-01-01 00:00:00

abstract：:Gibberellins (GAs) are a group of phytohormones that control many developmental processes in higher plants. We report the cloning and expression pattern of gibberellin biosynthesis genes from a new GA-producing fungus, Fusarium proliferatum (strain KGL0401). These genes sequences are deposited in the National Center f...

journal_title：Journal of microbiology and biotechnology

authors： Rim SO,You YH,Yoon H,Kim YE,Lee JH,Kang MS,Kim C,Seu YB,Kim JG

更新日期：2013-05-01 00:00:00

abstract：:Glucose dehydrogenase (GDH) is an oxidoreductase enzyme and is used as a biocatalyst to regenerate NAD(P)H in reductase-mediated chiral synthesis reactions. In this study, the glucose 1-dehydrogenase B gene (gdhB) was cloned from Bacillus thuringiensis subsp. kurstaki, and wild-type (GDH-BTWT) and His-tagged (GDH-BTN-...

journal_title：Journal of microbiology and biotechnology

authors： Hyun J,Abigail M,Choo JW,Ryu J,Kim HK

更新日期：2016-10-28 00:00:00

abstract：:Doenjang, a traditional Korean fermented soybean paste, is made by mixing and ripening meju with high salt brine (approximately 18%). Meju is a naturally fermented soybean block prepared by soaking, steaming, and molding soybean. To understand living bacterial community migration and the roles of bacteria in the manuf...

journal_title：Journal of microbiology and biotechnology

authors： Jeong DW,Kim HR,Jung G,Han S,Kim CT,Lee JH

更新日期：2014-05-01 00:00:00

abstract：:Hepatitis B core antigen (HBcAg) is an important serological marker used in the diagnosis of hepatitis B virus (HBV) infections. In the current study, a fast and efficient preparative purification protocol for truncated HBcAg from Escherichia coli disruptate was developed. The recombinant HBcAg was first captured by a...

journal_title：Journal of microbiology and biotechnology

authors： Ho CW,Tan WS,Chong FC,Ling TC,Tey BT

更新日期：2009-04-01 00:00:00

abstract：:A tightly regulated gene expression system composed of a single-copy target gene under the control of a lac promoter derivative and lacI gene in a multicopy plasmid is proposed, and its ability to control the flux of a metabolic pathway is demonstrated. A model system to control the flux of acetyl-CoA to acetyl phosph...

journal_title：Journal of microbiology and biotechnology

authors： Lee SG,Liao JC

更新日期：2008-02-01 00:00:00

abstract：:Activated sludge was sequentially adapted to benzene, toluene, and o-xylene (BTX) to study the effects on the change of microbial community. Sludge adapted to BTX separately degraded each by various rates in the following order; toluene>o-xylene>benzene. Degradation rates were increased after exposure to repeated spik...

journal_title：Journal of microbiology and biotechnology

authors： Park JY,Sang BI

更新日期：2007-11-01 00:00:00

abstract：:This study investigated the antibacterial activities of sophoraflavanone G from Sophora flavescens in combination with two antimicrobial agents against oral bacteria. The combined effect of sophoraflavanone G and the antimicrobial agents was evaluated using the checkerboard method to obtain a fractional inhibitory con...

journal_title：Journal of microbiology and biotechnology

authors： Cha JD,Jeong MR,Jeong SI,Lee KY

更新日期：2007-05-01 00:00:00

abstract：:To shed light on the genetic differences among food-originated coagulase-negative Staphylococcus (CNS), we performed pan-genome analysis of five species: Staphylococcus carnosus (two strains), Staphylococcus equorum (two strains), Staphylococcus succinus (three strains), Staphylococcus xylosus (two strains), and Staph...

journal_title：Journal of microbiology and biotechnology

authors： Heo S,Lee JS,Lee JH,Jeong DW

更新日期：2020-03-28 00:00:00

abstract：:An endocellulase-free multienzyme complex was produced by a thermophilic anaerobic bacterium, Thermoanaerobacterium thermosaccharolyticum strain NOI-1, when grown on xylan. The temperature and pH optima for growth were 60 degrees C and 6.0, respectively. The bacterial cells were found to adhere to insoluble xylan and ...

journal_title：Journal of microbiology and biotechnology

authors： Chimtong S,Tachaapaikoon C,Pason P,Kyu KL,Kosugi A,Mori Y,Ratanakhanokchai K

更新日期：2011-03-01 00:00:00

abstract：:Colorectal cancer (CRC) is regarded as one of the most common and deadly forms of cancer. Gut microbiota is vital to retain and promote several functions of intestinal. Although previous researches have shown that some gut microbiota have the abilities to inhibit tumorigenesis and prevent cancer from progressing, they...

journal_title：Journal of microbiology and biotechnology

authors： Chen C,Li H

更新日期：2020-11-28 00:00:00

abstract：:Total 121 lactic acid bacteria were isolated from homemade Inner Mongolia extra hard Hurood cheese. Seven of these strains, identified as Lactobacillus plantarum, were studied for probiotic characteristics. All seven strains survived at pH 3.0 for 3 h, or in the presence of oxgall at 0.3% or 0.6% for 4 h, but their vi...

journal_title：Journal of microbiology and biotechnology

authors： Zhang J,Zhang X,Zhang L,Zhao Y,Niu C,Yang Z,Li S

更新日期：2014-02-28 00:00:00

abstract：:Multidomain proteins for the biochemical analysis of the scouring efficiency of cotton fabrics were constructed by the fusion of a reporter moiety in the N-terminal and the cellulose binding domain (CBD) in the C-terminal. Based on the specific binding of the CBD of Cellulomonas fimi exoglucanase (Cex) to crystalline ...

journal_title：Journal of microbiology and biotechnology

authors： Ha JS,Lee YM,Choi SL,Song JJ,Shin CS,Kim JH,Lee SG

更新日期：2008-03-01 00:00:00

abstract：:The cohesin complex holds sister chromatids together and prevents premature chromosome segregation until the onset of anaphase. Mcd1 (also known as Scc1), the α-kleisin subunit of cohesin, is a key regulatory subunit of the mitotic cohesin complex and is required for maintaining sister chromatid cohesion, chromosome o...

journal_title：Journal of microbiology and biotechnology

authors： Lee MS,Yoon SW,Kim KP

更新日期：2015-05-01 00:00:00

abstract：:Accumulating evidence indicates that lactic acid bacteria could improve host physiology and lipid metabolism. To investigate the effect of the gut microbiota on host lipid metabolism, a hyperlipidemic rat model was established by feeding rats a high-fat diet for 28 days, and the gut microbiota of the rats was analyzed...

journal_title：Journal of microbiology and biotechnology

authors： Chen D,Yang Z,Chen X,Huang Y,Yin B,Guo F,Zhao H,Huang J,Wu Y,Gu R

更新日期：2015-05-01 00:00:00

abstract：:A beta-agarase gene, agaB34, was functionally cloned from the genomic DNA of a marine bacterium, Agarivorans albus YKW-34. The open reading frame of agaB34 consisted of 1,362 bp encoding 453 amino acids. The deduced amino acid sequence, consisting of a typical N-terminal signal peptide followed by a catalytic domain o...

journal_title：Journal of microbiology and biotechnology

authors： Fu XT,Pan CH,Lin H,Kim SM

更新日期：2009-03-01 00:00:00

abstract：:A 2.5 kb aga gene encoding alpha-galactosidase (alpha- Gal) from Leuconostoc mesenteroides SY1 was cloned into pSJE, an E. coli-Leuconostoc shuttle vector. The recombinant plasmid, pSJEaga, was introduced into Leuconostoc citreum KCTC3526 (ATCC49370) by electroporation. Transcription level of aga was the highest in ce...

journal_title：Journal of microbiology and biotechnology

authors： Park JY,Jeong SJ,Lee AR,Park JY,Jeong WJ,Kim JH

更新日期：2007-12-01 00:00:00

abstract：:Paenibacillus polymyxa is known to be a plant-growthpromoting rhizobacterium. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the specific detection and quantitation of P. polymyxa using a primer pair based on the sequence of a membranefusion protein for the amplification of a 268...

journal_title：Journal of microbiology and biotechnology

authors： Cho MS,Park DS,Lee JW,Chi HY,Sohn SI,Jeon BK,Ma JB

更新日期：2012-11-01 00:00:00

abstract：:Dephosphocoenzyme A (CoaE) catalyzes the last step in the biosynthesis of the cofactor coenzyme A. In this study, we report the identification and application of CoaE from Stretomyces peucetius ATCC27952. After expression of coaE, the protein was found to have a molecular mass of 28.6 kDa. Purification of the His-tagg...

journal_title：Journal of microbiology and biotechnology

authors： Lee NR,Rimal H,Lee JH,Oh TJ

更新日期：2014-09-01 00:00:00

abstract：:Leaves are the major biomass-producing organs in herbaceous plants and mainly develop during vegetative stage by activities of shoot apical meristem. There is a strong correlation between leaf number and bolting, a characteristic phenotype during the transition to reproductive phase in Arabidopsis thaliana. In order t...

journal_title：Journal of microbiology and biotechnology

authors： Lee BH

更新日期：2009-06-01 00:00:00

abstract：:Metarhizium anisopliae is a widely studied model to understand the virulence factors that participate in pathogenicity. Proteases such as subtilisin-like enzymes (Pr1) and trypsin-like enzymes (Pr2) are considered important factors for insect cuticle degradation. In four M. anisopliae strains (798, 6342, 6345, and 634...

journal_title：Journal of microbiology and biotechnology

authors： Rosas-García NM,Avalos-de-León O,Villegas-Mendoza JM,Mireles-Martínez M,Barboza-Corona JE,Castañeda-Ramírez JC

更新日期：2014-11-28 00:00:00

abstract：:The objectives of this study were to determine the antibiotypes, SCCmec subtypes, PVL carriage, and genetic diversity of MRSA strains from a tertiary hospital. Sixtysix MRSA strains were selected randomly (2003, 2004, and 2007) and tested for the Panton-Valentine leukocidin gene, mecA gene, and SCCmec type via a PCR. ...

journal_title：Journal of microbiology and biotechnology

authors： Thong KL,Junnie J,Liew FY,Yusof MY,Hanifah YA

更新日期：2009-10-01 00:00:00

abstract：:Mycophenolic acid (MPA) is an antibiotic produced by Penicillium brevicompactum. MPA has antifungal, antineoplastic, and immunosuppressive functions, among others. β-Hydroxy-β-methylglutaryl-CoA (HMG-CoA) lyase is a key enzyme in the bypass metabolic pathway. The inhibitory activity of HMG-CoA lyase increases the MPA ...

journal_title：Journal of microbiology and biotechnology

authors： Dong Y,Zhang J,Xu R,Lv X,Wang L,Sun A,Wei D

更新日期：2016-11-28 00:00:00

abstract：:Cryptomonads are unicellular, biflagellate algae. Generally, cryptomonad cells cannot be preserved well because of their fragile nature, and an improved methodology should be developed to identify cryptomonads from natural habitats. In this study, we tried using several cytological fixatives, including glutaraldehyde,...

journal_title：Journal of microbiology and biotechnology

authors： Xia S,Cheng Y,Zhu H,Liu G,Hu Z

更新日期：2013-03-01 00:00:00

abstract：:A Gram-negative, strictly aerobic, motile bacterial strain, designated Gsoil 124T, was isolated from a soil sample taken from a ginseng field in Pocheon Province (South Korea). The isolate contained Q-10 as the predominant lipoquinone, plus C18:1 7c and summed feature 4 (C16:1 6c and/or iso- C15:0 2-OH) as the major f...

journal_title：Journal of microbiology and biotechnology

authors： Lee HW,Ten IL,Jung HM,Liu QM,Im WT,Lee ST

更新日期：2008-06-01 00:00:00

abstract：:The abundance, biomass, size distribution, and taxonomic composition of bacterial and protistan (heterotrophic and autotrophic nanoflagellates and ciliates) communities were investigated in six lakes of Masurian Lake District (north-eastern Poland) differing in trophic state. Samples were taken from the trophogenic wa...

journal_title：Journal of microbiology and biotechnology

authors： Ryszard CJ,Tomasz A,Kalinowska K,Skowronska A

更新日期：2009-09-01 00:00:00