Anabolic effects of Peniel 2000, a peptide that regulates TGF-β1 signaling on intervertebral disc degeneration.

Abstract:

STUDY DESIGN:An in vitro study with bovine intervertebral disc (IVD) cells and an in vivo study with a rabbit disc degeneration model on the extracellular matrix metabolism by a biglycan-derived peptide (Peniel 2000; P2K). OBJECTIVE:To investigate the mechanism for P2K-induced increases in extracellular matrix and in vitro and in vivo effects of the peptide on IVD. SUMMARY OF BACKGROUND DATA:Transforming growth factor-β (TGF-β) has a functional versatility on the metabolism of IVD cells, suggesting that the regulation of TGF-β signaling is important in IVD degeneration. P2K was explored by an in silico drug discovery strategy to regulate TGF-β signaling. METHODS:The putative target of P2K was verified by Biacore 3000 analysis and affinity purification using biotin-P2K. A monolayer culture system of bovine IVD cells was used to demonstrate the mechanism underlying the anabolic effects of P2K. Smad signaling and extracellular matrix metabolism of the IVD cells were investigated by Western blot and reverse transcription-polymerase chain reaction, respectively. The in vivo effect of P2K on degenerated disc was investigated using a rabbit model of disc degeneration. In 14 New Zealand white rabbits, disc degeneration was induced by percutaneous annular punctures. After 4 weeks, 3 consecutive discs in the same animal were treated with 5% lactose or P2K per disc. Twelve weeks after the treatment, the regenerative activity in the disc was examined by radiography, magnetic resonance imaging, and biochemical and histological analyses. RESULTS:Direct binding of P2K to an active form of TGF-β1 was shown. Type II collagen and aggrecan were increased in TGF-β1/P2K-treated bovine IVD cells, compared with nontreated and TGF-β1-treated cells.In in vivo analysis, a single injection of P2K increased the disc height (P < 0.001) on the radiographs and improved the magnetic resonance imaging grade (P < 0.05) compared with controls. Biochemical analysis, showed a significant increase in PG content because of P2K treatment (P < 0.05). Histological analysis using disc degeneration grades demonstrated improvement in P2K-treated discs (P <0.01). CONCLUSION:A novel peptide, P2K, regulating TGF-β1 signaling had an anabolic effect on bovine IVD cells and rabbit degenerated discs. The results suggest that P2K has considerable potential as a treatment of degenerative disc disease.

journal_name

Spine (Phila Pa 1976)

journal_title

Spine

authors

Kwon YJ,Lee JW,Moon EJ,Chung YG,Kim OS,Kim HJ

doi

10.1097/BRS.0b013e31827aa896

subject

Has Abstract

pub_date

2013-01-15 00:00:00

pages

E49-58

issue

2

eissn

0362-2436

issn

1528-1159

journal_volume

38

pub_type

杂志文章

相关文献

SPINE文献大全
  • Changes in proteoglycans of intervertebral disc in diabetic patients. A possible cause of increased back pain.

    abstract:STUDY DESIGN:Characterization of the analytic profile of proteoglycans in the intervertebral discs at L4-L5 of nondiabetic (n = 5) and diabetic (n = 5) age-matched subjects. The discs used were discarded material from operations. OBJECTIVES:To clarify the reason for the higher risk of disc prolapse in diabetic patient...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/00007632-199804150-00001

    authors: Robinson D,Mirovsky Y,Halperin N,Evron Z,Nevo Z

    更新日期:1998-04-15 00:00:00

  • Low back pain. Epidemiologic aspects and work-related factors in the steel industry.

    abstract::A cross-sectional epidemiologic study about low back pain (LBP) reports and associated factors was conducted in two steel industries. A checklist was used to collect information concerning the health, the LBP history, the personal characteristics as well as the characteristics of the present and past working condition...

    journal_title:Spine

    pub_type: 杂志文章

    doi:

    authors: Masset D,Malchaire J

    更新日期:1994-01-15 00:00:00

  • The validity of compliance monitors to assess wearing time of thoracic-lumbar-sacral orthoses in children with spinal cord injury.

    abstract:STUDY DESIGN:Prospective multicenter observation. OBJECTIVE:To determine the validity of 3 commercially available at recording thoracic-lumbar-sacral orthosis (TLSO) wearing time of children with spinal cord injury (SCI) and to assess each monitor's function during daily activities. SUMMARY OF BACKGROUND DATA:A major...

    journal_title:Spine

    pub_type: 杂志文章,多中心研究

    doi:10.1097/BRS.0b013e318178864e

    authors: Hunter LN,Sison-Williamson M,Mendoza MM,McDonald CM,Molitor F,Mulcahey MJ,Betz RR,Vogel LC,Bagley A

    更新日期:2008-06-15 00:00:00

  • Spinal stability and intersegmental muscle forces. A biomechanical model.

    abstract::The human spinal column, devoid of musculature, is incapable of carrying normal physiologic loads. In an in vitro experiment, the effect of simulated intersegmental muscle forces on spinal instability was investigated. Intact and sequentially injured fresh lumbar functional spinal units were subjected to three-dimensi...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/00007632-198902000-00008

    authors: Panjabi M,Abumi K,Duranceau J,Oxland T

    更新日期:1989-02-01 00:00:00

  • Primary Epidural Lumbar Ewing Sarcoma: Case Report and Review of the Literature.

    abstract:STUDY DESIGN:Case report. OBJECTIVE:We present a case of isolated primary epidural lumbar Ewing sarcoma and review the current literature on the standard management. We also propose laminoplasty as safe procedure in this patient population that can provide good stabilization in young people. SUMMARY OF BACKGROUND DAT...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/BRS.0000000000001246

    authors: Giner J,Isla A,Cubedo R,Tejerina E

    更新日期:2016-03-01 00:00:00

  • Mobility of lumbar segments instrumented with a ProDisc II prosthesis: a two-year follow-up study.

    abstract:STUDY DESIGN:Longitudinal prospective study on a sample of 41 consecutive disc prosthesis patients, covering a postoperative time period of at least 2 years. OBJECTIVES:To document the rotational and translational range of segmental motion of patients instrumented with ProDisc II prostheses in the lumbar spine and to ...

    journal_title:Spine

    pub_type: 临床试验,杂志文章

    doi:10.1097/01.brs.0000224213.45330.68

    authors: Leivseth G,Braaten S,Frobin W,Brinckmann P

    更新日期:2006-07-01 00:00:00

  • Surgical management in 40 consecutive patients with cervical spinal epidural abscesses: shifting toward circumferential treatment.

    abstract:STUDY DESIGN:Retrospective database review of a prospectively maintained neurosurgical database. OBJECTIVE:The surgical management of cervical spinal epidural abscesses (CSEA) is reviewed examining the shift from single to staged anteroposterior decompression and stabilization. SUMMARY OF BACKGROUND DATA:CSEA managem...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/BRS.0000000000000942

    authors: Ghobrial GM,Viereck MJ,Margiotta PJ,Beygi S,Maulucci CM,Heller JE,Vaccaro AR,Harrop JS

    更新日期:2015-09-01 00:00:00

  • Coccygeal retroversion.

    abstract:STUDY DESIGN:A retrospective review of a case of coccygodynia caused by a previously unreported coccygeal morphology. OBJECTIVES:To draw attention to a previously undiagnosed pathology. BACKGROUND:This case adds to the recently described variants of coccygeal anatomy. METHODS:The authors present the case of a 42-yea...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/01.brs.0000127194.97062.17

    authors: Dennell LV,Nathan S

    更新日期:2004-06-15 00:00:00

  • Direct Repair of Lumbar Pars Interarticularis Defects by Utilizing Intraoperative O-Arm-Based Navigation and Microendoscopic Techniques.

    abstract:STUDY DESIGN:A retrospective analysis of the clinical outcomes of eight patients with the lumbar pars interarticulars defects treated by direct repair with the aid of intraoperative O-arm based navigation and microendoscopic techniques. OBJECTIVE:The aim of this study was to investigate the efficacy and safety of dire...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/BRS.0000000000001815

    authors: Jia M,Wang J,Zhang Z,Zheng W,Zhou Y

    更新日期:2016-10-01 00:00:00

  • Radiologic evaluation of adjacent superior segment facet joint violation following transpedicular instrumentation of the lumbar spine.

    abstract:STUDY DESIGN:The location of pedicle screws in relation to adjacent superior segment facet joints in 106 patients after lumbar spinal fusion was assessed using computed tomography and plain radiographs. OBJECTIVES:To document the incidence of adjacent superior segment facet joint violation following transpedicular ins...

    journal_title:Spine

    pub_type: 临床试验,杂志文章

    doi:10.1097/01.BRS.0000042361.93572.74

    authors: Shah RR,Mohammed S,Saifuddin A,Taylor BA

    更新日期:2003-02-01 00:00:00

  • Quantification of leakage pressures after durotomy repairs in the canine.

    abstract::This study was undertaken to investigate the relative strengths of dural repair using standard suture techniques, suture supplemented with tissue adhesive, and tissue adhesive alone. Uniform 2 mm dural defects were created in adult beagles, repaired, and then subjected to pressurization testing. Defects repaired with ...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/00007632-199009000-00024

    authors: Cain JE Jr,Rosenthal HG,Broom MJ,Jauch EC,Borek DA,Jacobs RR

    更新日期:1990-09-01 00:00:00

  • Anterior spinal fusion with Zielke instrumentation for idiopathic scoliosis. A frontal and sagittal curve analysis in 36 patients.

    abstract::The authors have reviewed the records of 36 patients with idiopathic scoliosis who underwent a single-stage anterior spinal fusion with Zielke instrumentation to determine if any adverse frontal or secondary sagittal deformities or changes in balance resulted from the surgery. Spontaneous correction of uninstrumented ...

    journal_title:Spine

    pub_type: 杂志文章

    doi:

    authors: Lowe TG,Peters JD

    更新日期:1993-03-15 00:00:00

  • Are We Underestimating the Significance of Pedicle Screw Misplacement?

    abstract:STUDY DESIGN:A retrospective review of charts, x-rays (XRs) and computed tomography (CT) scans was performed. OBJECTIVE:To evaluate the accuracy of pedicle screw placement using a novel classification system to determine potentially significant screw misplacement. SUMMARY OF BACKGROUND DATA:The accuracy rate of pedic...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/BRS.0000000000001318

    authors: Sarwahi V,Wendolowski SF,Gecelter RC,Amaral T,Lo Y,Wollowick AL,Thornhill B

    更新日期:2016-05-01 00:00:00

  • Cost-effectiveness of Balloon Kyphoplasty for Patients With Acute/Subacute Osteoporotic Vertebral Fractures in the Super-Aging Japanese Society.

    abstract:STUDY DESIGN:A propensity score matching study. OBJECTIVE:The aim of this study was to assess the cost-effectiveness of balloon kyphoplasty (BKP) in Japan. SUMMARY OF BACKGROUND DATA:Osteoporotic vertebral fracture (OVF) is a common disease in elderly people. In Japan, the incidence of painful OVF in 2008 was estimat...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/BRS.0000000000002829

    authors: Takahashi S,Hoshino M,Yasuda H,Terai H,Hayashi K,Tsujio T,Kono H,Suzuki A,Tamai K,Ohyama S,Toyoda H,Dohzono S,Kanematsu F,Hori Y,Nakamura H

    更新日期:2019-03-01 00:00:00

  • Factors associated with recurrent back pain and cyst recurrence after surgical resection of one hundred ninety-five spinal synovial cysts: analysis of one hundred sixty-seven consecutive cases.

    abstract:STUDY DESIGN:Retrospective study. OBJECTIVE:Compare outcomes of different treatment methods for intraspinal synovial cysts. SUMMARY OF BACKGROUND DATA:Intraspinal synovial cysts are cited as an increasing cause of back pain and radiculopathy. To date, few studies have compared outcomes of differing treatment methods ...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/BRS.0b013e3181bdafed

    authors: Xu R,McGirt MJ,Parker SL,Bydon M,Olivi A,Wolinsky JP,Witham TF,Gokaslan ZL,Bydon A

    更新日期:2010-05-01 00:00:00

  • The use of thoracoscopy in the management of adolescent idiopathic scoliosis.

    abstract:STUDY DESIGN:Retrospective case cohort series. OBJECTIVE:To analyze the outcomes of thoracoscopy in the surgical treatment of adolescent idiopathic scoliosis. SUMMARY OF BACKGROUND DATA:Traditionally, progressive idiopathic scoliosis has been treated surgically with either an open posterior, anterior, or combined sur...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/BRS.0b013e31815a51e3

    authors: Norton RP,Patel D,Kurd MF,Picetti GD,Vaccaro AR

    更新日期:2007-11-15 00:00:00

  • Mechanical stability of thoracolumbar pedicle screw fixation. The effect of crosslinks.

    abstract:STUDY DESIGN:Pedicle screw fixation for unstable thoracolumbar spine injuries is relatively new. The effect of one or two crosslinks on rotational and lateral bending stiffness was studied. OBJECTIVE:To determine the rotational and bending stiffness values of thoracolumbar fractures fixed by the AO's internal fixation...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/00007632-199707150-00007

    authors: Lynn G,Mukherjee DP,Kruse RN,Sadasivan KK,Albright JA

    更新日期:1997-07-15 00:00:00

  • Effect of type II odontoid fracture nonunion on outcome among elderly patients treated without surgery: based on the AOSpine North America geriatric odontoid fracture study.

    abstract:STUDY DESIGN:Subgroup analysis of a prospective multicenter study. OBJECTIVE:Outcome analysis of nonoperatively treated elderly patients with type II odontoid fractures, including assessment of consequence of a fracture nonunion. SUMMARY OF BACKGROUND DATA:Odontoid fractures are among the most common fractures in the...

    journal_title:Spine

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1097/BRS.0000000000000009

    authors: Smith JS,Kepler CK,Kopjar B,Harrop JS,Arnold P,Chapman JR,Fehlings MG,Vaccaro AR,Shaffrey CI

    更新日期:2013-12-15 00:00:00

  • The symptom of night pain in a back pain triage clinic.

    abstract:STUDY DESIGN:Prospective longitudinal study of patients attending a back pain triage clinic with night pain. OBJECTIVE:To assess the importance of the symptom of night pain in patients attending a back pain triage clinic. SUMMARY OF BACKGROUND DATA:The 1994 US Agency for Health Care Policy and Research guidelines sug...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/01.brs.0000176313.02258.5e

    authors: Harding IJ,Davies E,Buchanan E,Fairbank JT

    更新日期:2005-09-01 00:00:00

  • Inpatient Pain Scores and Narcotic Utilization Based on American Society of Anesthesiologists Score Following Anterior Cervical Discectomy and Fusion.

    abstract:STUDY DESIGN:Retrospective. OBJECTIVE:To examine the relationship between American Society of Anesthesiologists (ASA) score and inpatient pain and narcotics consumption following anterior cervical discectomy and fusion (ACDF). SUMMARY OF BACKGROUND DATA:Higher ASA scores have been previously linked to increased posto...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/BRS.0000000000003135

    authors: Yoo JS,Brundage TS,Hrynewycz NM,Singh K

    更新日期:2019-11-15 00:00:00

  • Bone mineral density in patients with ossification of the posterior longitudinal ligament. Minimal decrease of bone mineral density with aging.

    abstract:STUDY DESIGN:Bone mineral density of individuals with ossification of the posterior longitudinal ligament and that of normal people was determined by dual-energy x-ray absorptiometry. OBJECTIVES:To determine whether bone mineral density in the people with ossification of the posterior longitudinal ligament is higher t...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/00007632-199710150-00013

    authors: Mamada T,Nakamura K,Hoshino Y,Saita K,Kurokawa T

    更新日期:1997-10-15 00:00:00

  • A new in vivo animal model to create intervertebral disc degeneration characterized by MRI, radiography, CT/discogram, biochemistry, and histology.

    abstract:STUDY DESIGN:A new in vivo sheep model was developed that produced disc degeneration through the injection of 5-bromodeoxyuridine (BrdU) into the intervertebral disc. This process was studied using magnetic resonance imaging (MRI), radiography, CT/discogram, histology, and biochemistry. OBJECTIVES:To develop a sheep m...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/01.brs.0000259835.31062.3d

    authors: Zhou H,Hou S,Shang W,Wu W,Cheng Y,Mei F,Peng B

    更新日期:2007-04-15 00:00:00

  • Posterior spinal artery infarct due to patent foramen ovale: a case report.

    abstract:STUDY DESIGN:Case report. OBJECTIVE:To report the first case of posterior spinal artery (PSA) infarct due to patent foramen ovale (PFO). SUMMARY OF BACKGROUND DATA:Infarct in the territories of PSA are very rare: till now 38 cases are reported in the literature. Moreover only 1 case of spinal cord infarction was attr...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/BRS.0b013e3181cf29ea

    authors: Petruzzellis M,Fraddosio A,Giorelli M,Prontera M,Tinelli A,Lucivero V,Federico F

    更新日期:2010-03-01 00:00:00

  • Delayed granulocyte colony-stimulating factor treatment in rats attenuates mechanical allodynia induced by chronic constriction injury of the sciatic nerve.

    abstract:STUDY DESIGN:Animal experimental study with intervention. OBJECTIVE:The aim of this study was to elucidate therapeutic effects of delayed granulocyte colony-stimulating factor treatment for mechanical allodynia induced by chronic constriction injury (CCI) of the sciatic nerve in rats. SUMMARY OF BACKGROUND DATA:Granu...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/BRS.0000000000000108

    authors: Koda M,Furuya T,Kato K,Mannoji C,Hashimoto M,Inada T,Kamiya K,Ota M,Maki S,Okawa A,Takahashi K,Ishikawa T,Yamazaki M

    更新日期:2014-02-01 00:00:00

  • Repositioning error in low back pain. Comparing trunk repositioning error in subjects with chronic low back pain and control subjects.

    abstract:STUDY DESIGN:Repositioning error of the trunk was tested in 20 subjects with chronic low back pain and in 20 control subjects. The 3Space Tracker (Polhemus, Colchester, VT), a device that measures three-dimensional position in space, was used to determine the subject's trunk position. OBJECTIVES:To determine whether r...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/00007632-200001150-00017

    authors: Newcomer K,Laskowski ER,Yu B,Larson DR,An KN

    更新日期:2000-01-15 00:00:00

  • Influence of sacral morphology in developmental spondylolisthesis.

    abstract:STUDY DESIGN:A radiographic study was conducted to investigate sacral morphology in a children and adolescent population with developmental L5-S1 spondylolisthesis. OBJECTIVE:To determine the relationship between sacral morphology and developmental L5-S1 spondylolisthesis. SUMMARY AND BACKGROUND DATA:The morphology o...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/BRS.0b013e3181857f70

    authors: Wang Z,Parent S,Mac-Thiong JM,Petit Y,Labelle H

    更新日期:2008-09-15 00:00:00

  • Studies in the modified Scoliosis Research Society Outcomes Instrument in adults: validation, reliability, and discriminatory capacity.

    abstract:STUDY DESIGN:Observational study of patients with scoliosis and matched controls. OBJECTIVES:To determine the validity and reliability of the modified Scoliosis Research Society Outcomes Instrument (SRS-22) for use in the assessment of deformity in adults. To demonstrate the discriminate validity of the SRS-22 in diff...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/01.BRS.0000084666.53553.D6

    authors: Berven S,Deviren V,Demir-Deviren S,Hu SS,Bradford DS

    更新日期:2003-09-15 00:00:00

  • Functional morphology of the thoracolumbar transversospinal muscles.

    abstract::STUDY DESIGN. A qualitative and semiquantitative study of the morphology of the human thoracolumbar transversospinal (TSP) muscles. OBJECTIVE. To further define the functional morphology of the thoracolumbar TSP muscles. SUMMARY OF BACKGROUND DATA. The TSP muscle group plays an important role in vertebral function but...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/BRS.0b013e3181f79629

    authors: Cornwall J,Stringer MD,Duxson M

    更新日期:2011-07-15 00:00:00

  • Sagittal translation in opening wedge osteotomy for the correction of thoracolumbar kyphotic deformity in ankylosing spondylitis.

    abstract:STUDY DESIGN:Retrospective. OBJECTIVES:To document sagittal translation (ST) of the vertebral column in opening wedge osteotomy (OWO) for ankylosing spondylitis (AS) kyphosis. SUMMARY OF BACKGROUND DATA:Surgeons recognize displacement at the hinge of correction in OWO as an accidental event between the vertebrae on t...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/01.brs.0000216479.09250.96

    authors: Chang KW,Chen HC,Chen YY,Lin CC,Hsu HL,Cai YH

    更新日期:2006-05-01 00:00:00

  • Relative safety of anterior microsurgical decompression versus laminoplasty for cervical myelopathy with a massive ossified posterior longitudinal ligament.

    abstract:STUDY DESIGN:A retrospective clinical study was conducted. OBJECTIVE:To investigate the relative safety of anterior microsurgical decompression with iliac strut graft fusion versus laminoplasty for a massive ossified posterior longitudinal ligament in the cervical spine. SUMMARY OF BACKGROUND DATA:The question of how...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/00007632-200211150-00013

    authors: Tani T,Ushida T,Ishida K,Iai H,Noguchi T,Yamamoto H

    更新日期:2002-11-15 00:00:00