Effect of heparin-derived oligosaccharide on vascular smooth muscle cell proliferation.

Abstract:

:In this study, the effect of heparin-derived oligosaccharide on bovine vascular smooth muscle cell (VSMC) proliferation and signal transduction mechanism was investigated. Extracellular-signal-regulated kinase (ERK) 1/2 has been implicated in the regulation of various cellular functions including proliferation, and we sought to define a functional role for ERK 1/2 in an established proliferation model in order to find a possible mechanism for inhibition of VSMC proliferation by heparin-derived oligosaccharide. The VSMC proliferation model was developed by platelet-derived growth factor (PDGF), and the level of ERK 1/2 protein and messenger RNA was determined by reverse transcriptase-polymerase chain reaction, Western blotting, and immunocytochemical methods. Flow cytometry analysis indicated that heparin-derived oligosaccharide blocked PDGF-induced cell cycle progression by arresting cells in the G0/G1 phase. The results imply that heparin-derived oligosaccharide inhibits VSMC proliferation by moderating the gene and the phosphorylation levels of ERK 1/2, eventually blocking G1/S transition, may be one of the mechanisms for inhibition of VSMC proliferation by heparin-derived oligosaccharide.

journal_name

Vasc Endovascular Surg

authors

Li L,Li W,Ren Z,He S,Xu G,Yang L

doi

10.1177/1538574412442595

subject

Has Abstract

pub_date

2012-07-01 00:00:00

pages

393-400

issue

5

eissn

1538-5744

issn

1938-9116

pii

1538574412442595

journal_volume

46

pub_type

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