Abstract:
:Burkholderia pseudomallei and B. mallei are two highly pathogenic bacteria responsible for melioidosis and glanders, respectively. Our laboratory developed hydrolysis probe-based real-time polymerase chain reaction assays targeting type three secretion system (TTS) and transposase family protein (TFP) of B. pseudomallei and B. malli, respectively. The assays were validated for target specificity, amplification sensitivity, and reproducibility. A bacterial DNA panel, composed of B. pseudomallei (13 strains), B. mallei (11 strains), Burkholderia species close neighbors (5 strains), and other bacterial species (17 strains), was prepared for specificity testing. Reference DNAs from B. pseudomallei and B. mallei bacterial cultures were used as controls for amplification, limit of detection, and reproducibility testing. The two TaqMan assays, Bp-TTS 1 and Bm-TFP, were optimized and applied in a retrospective study of archived cases from the Armed Forces Institute of Pathology. We tested 10 formalin-fixed paraffin-embedded blocks originally from autopsy specimens of patients who died of melioidosis or glanders during or after overseas tours in 1960s. Polymerase chain reaction results confirmed that DNA samples from formalin-fixed paraffin-embedded blocks of eight patients with melioidosis were positive for Bp-TTS 1 target and two patients with glanders were positive for Bm-TFP target.
journal_name
Mil Medjournal_title
Military medicineauthors
Zhang B,Wear DJ,Kim HS,Weina P,Stojadinovic A,Izadjoo Mdoi
10.7205/milmed-d-11-00232subject
Has Abstractpub_date
2012-02-01 00:00:00pages
216-21issue
2eissn
0026-4075issn
1930-613Xjournal_volume
177pub_type
杂志文章abstract:OBJECTIVES:Military medical subspecialists consult with referring physicians, direct patient care and education, support isolated subspecialists, and maintain cohesive readiness plans. The World Wide Web appears promising in fulfilling these roles. METHODS:A website providing directed resources, the Walter Reed Army M...
journal_title:Military medicine
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doi:
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abstract::From April 24 to 26, 2013, the Armed Forces Health Surveillance Center and the U.S. Africa Command cosponsored the inaugural meeting of the West Africa Malaria Task Force in Accra, Ghana. The meeting's purpose was to identify common challenges, explore regional and transcontinental collaborations, and to share knowled...
journal_title:Military medicine
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journal_title:Military medicine
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journal_title:Military medicine
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doi:
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更新日期:2015-04-01 00:00:00
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journal_title:Military medicine
pub_type: 历史文章,杂志文章
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更新日期:2011-06-01 00:00:00
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journal_title:Military medicine
pub_type: 杂志文章
doi:
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journal_title:Military medicine
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
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更新日期:2013-05-01 00:00:00
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journal_title:Military medicine
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doi:
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journal_title:Military medicine
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doi:
更新日期:1991-02-01 00:00:00
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journal_title:Military medicine
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
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journal_title:Military medicine
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doi:
更新日期:2002-06-01 00:00:00
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更新日期:2011-04-01 00:00:00
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doi:
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更新日期:2006-08-01 00:00:00
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