当前位置： SCI文献检索 > MOLECULAR PHARMACEUTICS期刊下所有文献 > Localized hydration in lyophilized myoglobin by hydrogen-deuterium exchange mass spectrometry. 2. Exchange kinetics.

Localized hydration in lyophilized myoglobin by hydrogen-deuterium exchange mass spectrometry. 2. Exchange kinetics.

Abstract：

:Solid-state hydrogen-deuterium exchange with mass spectrometric analysis (ssHDX) is a promising method for characterizing proteins in amorphous solids. Though analysis of HDX kinetics is informative and well-established in solution, application of these methods to solid samples is complicated by possible heterogeneities in the solid. The studies reported here provide a detailed analysis of the kinetics of hydration and ssHDX for equine myoglobin (Mb) in solid matrices containing sucrose or mannitol. Water sorption was rapid relative to ssHDX, indicating that ssHDX kinetics was not limited by bulk water transport. Deuterium uptake in solids was well-characterized by a biexponential model; values for regression parameters provided insight into differences between the two solid matrices. Analysis of the widths of peptide mass envelopes revealed that, in solution, an apparent EX2 mechanism prevails, consistent with native conformation of the protein. In contrast, in mannitol-containing samples, a smaller non-native subpopulation exchanges by an EX1-like mechanism. Together, the results indicate that the analysis of ssHDX kinetic data and of the widths of peptide mass envelopes is useful in screening solid formulations of protein drugs for the presence of non-native species that cannot be detected by amide I FTIR.

journal_name

Mol Pharm

journal_title

Molecular pharmaceutics

authors

Sophocleous AM,Topp EM

doi

10.1021/mp2004093

subject

Has Abstract

pub_date

2012-04-02 00:00:00

pages

727-33

issue

4

eissn

1543-8384

issn

1543-8392

journal_volume

9

pub_type

杂志文章

相关文献

MOLECULAR PHARMACEUTICS文献大全
  • Retro-inverso isomer of Angiopep-2: a stable d-peptide ligand inspires brain-targeted drug delivery.

    abstract：:The blood-brain barrier (BBB) prevents most drugs from reaching the site of central nervous system (CNS) diseases, intensively confining the therapeutic efficiency. Angiopep-2 (here termed (L)Angiopep), which is a 19-mer peptide derived from human Kunitz domain, can trigger transcytosis and traverse the BBB by recogni...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/mp500086e

    authors： Wei X,Zhan C,Chen X,Hou J,Xie C,Lu W

    更新日期：2014-10-06 00:00:00

  • Enhanced antiproliferative activity of transferrin-conjugated paclitaxel-loaded nanoparticles is mediated via sustained intracellular drug retention.

    abstract：:We studied the molecular mechanism of greater efficacy of paclitaxel-loaded nanoparticles (Tx-NPs) following conjugation to transferrin (Tf) ligand in breast cancer cell line. NPs were formulated using biodegradable polymer, poly(lactic-co-glycolide) (PLGA), with encapsulated Tx and conjugated to Tf ligand via an epox...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/mp050032z

    authors： Sahoo SK,Labhasetwar V

    更新日期：2005-09-01 00:00:00

  • Physicochemical characterization of molecular assemblies of miltefosine and amphotericin B.

    abstract：:This study describes the interactions between two amphiphilic molecules with antileishmanial activity, amphotericin B (AmB) and miltefosine [hexadecylphosphocholine (HePC)], the latter being effective by the oral route. The effect of HePC on the aggregation state of AmB in aqueous solution and the interactions between...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/mp0601143

    authors： Ménez C,Legrand P,Rosilio V,Lesieur S,Barratt G

    更新日期：2007-03-01 00:00:00

  • Increasing the Net Negative Charge by Replacement of DOTA Chelator with DOTAGA Improves the Biodistribution of Radiolabeled Second-Generation Synthetic Affibody Molecules.

    abstract：:A promising strategy to enable patient stratification for targeted therapies is to monitor the target expression in a tumor by radionuclide molecular imaging. Affibody molecules (7 kDa) are nonimmunoglobulin scaffold proteins with a 25-fold smaller size than intact antibodies. They have shown an apparent potential as ...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/acs.molpharmaceut.6b00089

    authors： Westerlund K,Honarvar H,Norrström E,Strand J,Mitran B,Orlova A,Eriksson Karlström A,Tolmachev V

    更新日期：2016-05-02 00:00:00

  • Engineered Human Adipose Stem-Cell-Derived Exosomes Loaded with miR-21-5p to Promote Diabetic Cutaneous Wound Healing.

    abstract：:Diabetic wounds are a worldwide health problem causing extremely heavy public health burden and require effective treatment. Optimal strategies for treating nonhealing diabetic wounds include stem-cell-based therapy and delivery of novel drug substances, such as functional microRNAs (miRNAs); however, miRNA easily deg...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/acs.molpharmaceut.0c00177

    authors： Lv Q,Deng J,Chen Y,Wang Y,Liu B,Liu J

    更新日期：2020-05-04 00:00:00

  • Targeted and Synergic Glioblastoma Treatment: Multifunctional Nanoparticles Delivering Verteporfin as Adjuvant Therapy for Temozolomide Chemotherapy.

    abstract：:Despite advances in cancer therapies, glioblastoma multiforme treatment remains inefficient due to the brain-blood barrier (BBB) inhibitory activity and to the low temozolomide (TMZ) chemotherapeutic selectivity. To improve therapeutic outcomes, in this work we propose two strategies, (i) photodynamic therapy (PDT) as...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/acs.molpharmaceut.8b01001

    authors： Pellosi DS,Paula LB,de Melo MT,Tedesco AC

    更新日期：2019-03-04 00:00:00

  • Post-PEGylation of siRNA Lipo-oligoamino Amide Polyplexes Using Tetra-glutamylated Folic Acid as Ligand for Receptor-Targeted Delivery.

    abstract：:For efficient and receptor-specific siRNA delivery, a new post-PEGylation strategy was established to provide siRNA polyplexes with targeting and shielding agents. For this purpose, core nanoparticles were formed by complexing siRNA with sequence-defined cationic lipo-oligomers. The T-shaped bis-oleoyl-oligoethanamino...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/acs.molpharmaceut.6b00102

    authors： Müller K,Kessel E,Klein PM,Höhn M,Wagner E

    更新日期：2016-07-05 00:00:00

  • Side Chain Optimization Remarkably Enhances the in Vivo Stability of 18F-Labeled Glutamine for Tumor Imaging.

    abstract：:Similar to glycolysis, glutaminolysis acts as a vital energy source in tumor cells, providing building blocks for the metabolic needs of tumor cells. To capture glutaminolysis in tumors, 18F-(2S,4R)4-fluoroglutamine ([18F]FGln) and 18F-fluoroboronoglutamine ([18F]FBQ) have been successfully developed for positron emis...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/acs.molpharmaceut.9b00891

    authors： Chen J,Li C,Hong H,Liu H,Wang C,Xu M,Han Y,Liu Z

    更新日期：2019-12-02 00:00:00

  • Factors Governing P-Glycoprotein-Mediated Drug-Drug Interactions at the Blood-Brain Barrier Measured with Positron Emission Tomography.

    abstract：:The adenosine triphosphate-binding cassette transporter P-glycoprotein (ABCB1/Abcb1a) restricts at the blood-brain barrier (BBB) brain distribution of many drugs. ABCB1 may be involved in drug-drug interactions (DDIs) at the BBB, which may lead to changes in brain distribution and central nervous system side effects o...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/acs.molpharmaceut.5b00168

    authors： Wanek T,Römermann K,Mairinger S,Stanek J,Sauberer M,Filip T,Traxl A,Kuntner C,Pahnke J,Bauer F,Erker T,Löscher W,Müller M,Langer O

    更新日期：2015-09-08 00:00:00

  • Controlling the Release of Indomethacin from Glass Solutions Layered with a Rate Controlling Membrane Using Fluid-Bed Processing. Part 1: Surface and Cross-Sectional Chemical Analysis.

    abstract：:Fluid bed coating has been shown to be a suitable manufacturing technique to formulate poorly soluble drugs in glass solutions. Layering inert carriers with a drug-polymer mixture enables these beads to be immediately filled into capsules, thus avoiding additional, potentially destabilizing, downstream processing. In ...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/acs.molpharmaceut.6b01023

    authors： Dereymaker A,Scurr DJ,Steer ED,Roberts CJ,Van den Mooter G

    更新日期：2017-04-03 00:00:00

  • Targeting of injectable drug nanocrystals.

    abstract：:"Nano" drug delivery carriers are established technologies for improving the therapeutic index of chemotherapeutic drugs and overcoming formulation challenges of poorly water-soluble compounds. Two important remaining challenges, however, are the need to formulate drugs on a case-by-case basis (due to the specific che...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章,评审

    doi：10.1021/mp5001247

    authors： Fuhrmann K,Gauthier MA,Leroux JC

    更新日期：2014-06-02 00:00:00

  • Assessment of a modular transfection system based upon cellular localization of DNA.

    abstract：:Delivery of plasmid DNA for protein production in mammalian cells is not an efficient process. In this study, the theory that cellular localization of plasmid DNA increases transfection efficiency is examined with an emphasis on the understanding of the cellular association of the components of a ternary transfection ...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/mp049969a

    authors： Guo C,Gemeinhart RA

    更新日期：2004-07-01 00:00:00

  • The biowaiver extension for BCS class III drugs: the effect of dissolution rate on the bioequivalence of BCS class III immediate-release drugs predicted by computer simulation.

    abstract：:The Biopharmaceutical Classification System (BCS) guidance issued by the FDA allows waivers for in vivo bioavailability and bioequivalence studies for immediate-release (IR) solid oral dosage forms only for BCS class I drugs. However, a number of drugs within BCS class III have been proposed to be eligible for biowaiv...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/mp100053q

    authors： Tsume Y,Amidon GL

    更新日期：2010-08-02 00:00:00

  • Dual Functional Peptide-Driven Nanoparticles for Highly Efficient Glioma-Targeting and Drug Codelivery.

    abstract：:Compared with peripheral tumors, glioma is very difficult to treat, not only because it has general features of tumor but also because the therapy has been restricted by the brain-blood barrier (BBB). The two main features of tumor growth are angiogenesis and proliferation of tumor cells. RNA interference (RNAi) can d...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/acs.molpharmaceut.6b00051

    authors： Kuang Y,Jiang X,Zhang Y,Lu Y,Ma H,Guo Y,Zhang Y,An S,Li J,Liu L,Wu Y,Liang J,Jiang C

    更新日期：2016-05-02 00:00:00

  • Biodistribution of fluorescently labeled PAMAM dendrimers in neonatal rabbits: effect of neuroinflammation.

    abstract：:Dendrimers are being explored in many preclinical studies as drug, gene, and imaging agent delivery systems. Understanding their detailed organ, tissue, cellular uptake, and retention can provide valuable insights into their effectiveness as delivery vehicles and the associated toxicity. This work explores a fluoresce...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/mp400371r

    authors： Lesniak WG,Mishra MK,Jyoti A,Balakrishnan B,Zhang F,Nance E,Romero R,Kannan S,Kannan RM

    更新日期：2013-12-02 00:00:00

  • Oral Delivery of β-Lactoglobulin-Nanosphere-Encapsulated Resveratrol Alleviates Inflammation in Winnie Mice with Spontaneous Ulcerative Colitis.

    abstract：:Resveratrol (RES) is a nutraceutical with promising anti-inflammatory properties for the treatment of inflammatory bowel diseases (IBD). However, the clinical effectiveness of resveratrol as an oral anti-inflammatory agent is hindered by its extremely poor solubility and poor stability. In this study, we encapsulated ...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/acs.molpharmaceut.0c00048

    authors： Pujara N,Wong KY,Qu Z,Wang R,Moniruzzaman M,Rewatkar P,Kumeria T,Ross BP,McGuckin M,Popat A

    更新日期：2021-02-01 00:00:00

  • Development of a poly(d,l-lactic-co-glycolic acid) nanoparticle formulation of STAT3 inhibitor JSI-124: implication for cancer immunotherapy.

    abstract：:Constitutively activated signal transducer and activator of transcription-3 (STAT3) in tumor and dendritic cells (DCs) plays a critical role in tumor-induced immunosuppression. This is considered a major challenge in effective immunotherapy of cancer. Herein we describe the development of a polymeric nanocarrier for t...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/mp900145g

    authors： Molavi O,Mahmud A,Hamdy S,Hung RW,Lai R,Samuel J,Lavasanifar A

    更新日期：2010-04-05 00:00:00

  • Active targeting of block copolymer micelles with trastuzumab Fab fragments and nuclear localization signal leads to increased tumor uptake and nuclear localization in HER2-overexpressing xenografts.

    abstract：:Block copolymer micelles (BCMs) have been employed as effective drug delivery systems to solid tumors by virtue of their capacity to transport large therapeutic payloads and passively target tumor sites. Active targeting of nanoparticles (NPs) has been exploited as a means to increase the therapeutic efficacy of NP-ba...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/mp400315p

    authors： Hoang B,Ekdawi SN,Reilly RM,Allen C

    更新日期：2013-11-04 00:00:00

  • Enhancing Oral Bioavailability of Cyclic RGD Hexa-peptides by the Lipophilic Prodrug Charge Masking Approach: Redirection of Peptide Intestinal Permeability from a Paracellular to Transcellular Pathway.

    abstract：:Hydrophilic peptides constitute most of the active peptides. They mostly permeate via tight junctions (paracellular pathway) in the intestine. This permeability mechanism restricts the magnitude of their oral absorption and bioavailability. We hypothesized that concealing the hydrophilic residues of the peptide using ...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/acs.molpharmaceut.8b00466

    authors： Schumacher-Klinger A,Fanous J,Merzbach S,Weinmüller M,Reichart F,Räder AFB,Gitlin-Domagalska A,Gilon C,Kessler H,Hoffman A

    更新日期：2018-08-06 00:00:00

  • Internalization of mRNA lipoplexes by dendritic cells.

    abstract：:Lipoplexes, composed of Lipofectamine and mRNA encoding HIV Gag protein, were shown to be internalized by dendritic cells (DCs) and promote antigen presentation to stimulate HIV-specific T cell responses. Using confocal microscopy, we showed that one-third of fluorescently labeled mRNA containing lipoplexes are coloca...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/mp3003336

    authors： De Haes W,Van Mol G,Merlin C,De Smedt SC,Vanham G,Rejman J

    更新日期：2012-10-01 00:00:00

  • Quantitative Imaging of the Hypoxia-Related Marker CAIX in Head and Neck Squamous Cell Carcinoma Xenograft Models.

    abstract：:Tumor hypoxia plays a major role in radio- and chemotherapy resistance in solid tumors. Carbonic Anhydrase IX (CAIX) is an endogenous hypoxia-related protein, which is associated with poor patient outcome. The quantitative assessment of CAIX expression of tumors may steer cancer treatment by predicting therapy respons...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/acs.molpharmaceut.8b00950

    authors： Huizing FJ,Hoeben BAW,Franssen GM,Boerman OC,Heskamp S,Bussink J

    更新日期：2019-02-04 00:00:00

  • Differential cytosolic delivery and presentation of antigen by listeriolysin O-liposomes to macrophages and dendritic cells.

    abstract：:Delivery of antigenic protein to the cytosol of antigen-presenting cells (APCs), such as macrophages (MPhi) and dendritic cells (DCs), is required for an efficient CD8 T-cell-mediated immune response. We have previously shown that co-encapsulation of antigenic protein inside pH-sensitive liposomes with listeriolysin O...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/mp049896v

    authors： Stier EM,Mandal M,Lee KD

    更新日期：2005-01-01 00:00:00

  • Hierarchical Mass Transfer Analysis of Drug Particle Dissolution, Highlighting the Hydrodynamics, pH, Particle Size, and Buffer Effects for the Dissolution of Ionizable and Nonionizable Drugs in a Compendial Dissolution Vessel.

    abstract：:Dissolution is a crucial process for the oral delivery of drug products. Before being absorbed through epithelial cell membranes to reach the systemic circulation, drugs must first dissolve in the human gastrointestinal (GI) tract. In vivo and in vitro dissolutions are complex because of their dependency upon the drug...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/acs.molpharmaceut.0c00614

    authors： Salehi N,Al-Gousous J,Mudie DM,Amidon GL,Ziff RM,Amidon GE

    更新日期：2020-10-05 00:00:00

  • Cyclopamine attenuates acute warm ischemia reperfusion injury in cholestatic rat liver: hope for marginal livers.

    abstract：:Cholestasis is a significant risk factor for immediate hepatic failure due to ischemia reperfusion (I/R) injury in patients undergoing liver surgery or transplantation. We recently demonstrated that inhibition of Hedgehog (Hh) signaling with cyclopamine (CYA) before I/R prevents liver injury. In this study we hypothes...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/mp200115v

    authors： Pratap A,Panakanti R,Yang N,Lakshmi R,Modanlou KA,Eason JD,Mahato RI

    更新日期：2011-06-06 00:00:00

  • Influence of microemulsion-mucin interaction on the fate of microemulsions diffusing through pig gastric mucin solutions.

    abstract：:Mucus layer, a selective diffusion barrier, has an important effect on the fate of drug delivery systems in the gastrointestinal tract. To study the fate of microemulsions in the mucus layer, four microemulsion formulations with different particle sizes and lipid compositions were prepared. The microemulsion-mucin int...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/mp500475y

    authors： Zhang J,Lv Y,Wang B,Zhao S,Tan M,Lv G,Ma X

    更新日期：2015-03-02 00:00:00

  • Selective Photokilling of Colorectal Tumors by Near-Infrared Photoimmunotherapy with a GPA33-Targeted Single-Chain Antibody Variable Fragment Conjugate.

    abstract：:Antibody-based near-infrared photoimmunotherapy (NIR-PIT) is an attractive strategy for cancer treatment. Tumor cells can be selectively and efficiently killed by the targeted delivery of an antibody-photoabsorber complex followed by exposure to NIR light. Glycoprotein A33 antigen (GPA33) is highly expressed in most h...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/acs.molpharmaceut.0c00210

    authors： Wei D,Tao Z,Shi Q,Wang L,Liu L,She T,Yi Q,Wen X,Liu L,Li S,Yang H,Jiang X

    更新日期：2020-07-06 00:00:00

  • Microgel Bioreactors for Cancer Cell Targeting by pH-Dependent Generation of Radicals.

    abstract：:The lack of specificity of traditional cytostatics and increasing resistance of cancer cells represent important challenges in cancer therapy. One of the characteristics of cancer cells is their intrinsic oxidative stress caused by higher metabolic activity, mitochondrial malfunction, and oncogene stimulation. This fe...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/acs.molpharmaceut.9b00531

    authors： Majerská M,Jakubec M,Klimša V,Rimpelová S,Král V,Štěpánek F

    更新日期：2019-07-01 00:00:00

  • Liver imaging with a novel hepatitis B surface protein derived SPECT-tracer.

    abstract：:Diagnostic imaging of the liver by ultrasound, computed tomography (CT) and magnetic resonance tomography (MRT) is generally limited to the visualization of the morphology. In order to exploit the intriguing liver tropism of the human hepatitis B virus (HBV) for molecular imaging of the liver, peptidic tracers derived...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/mp400038r

    authors： Müller T,Mehrle S,Schieck A,Haberkorn U,Urban S,Mier W

    更新日期：2013-06-03 00:00:00

  • SIRT1-mediated FoxO1 deacetylation is essential for multidrug resistance-associated protein 2 expression in tamoxifen-resistant breast cancer cells.

    abstract：:Our previous studies have shown that multidrug resistance protein 2 (MRP2) is overexpressed in tamoxifen-resistant MCF-7 breast cancer cells (TAMR-MCF-7 cells) and forkhead box-containing protein, O subfamily1 (FoxO1), functions as a key regulator of multidrug resistance 1 (MDR1) gene transcription. This study aimed t...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/mp400287p

    authors： Choi HK,Cho KB,Phuong NT,Han CY,Han HK,Hien TT,Choi HS,Kang KW

    更新日期：2013-07-01 00:00:00

  • Interactions of microbicide nanoparticles with a simulated vaginal fluid.

    abstract：:The interaction with cervicovaginal mucus presents the potential to impact the performance of drug nanocarriers. These systems must migrate through this biological fluid in order to deliver their drug payload to the underlying mucosal surface. We studied the ability of dapivirine-loaded polycaprolactone (PCL)-based na...

    journal_title：Molecular pharmaceutics

    pub_type： 杂志文章

    doi：10.1021/mp300408m

    authors： das Neves J,Rocha CM,Gonçalves MP,Carrier RL,Amiji M,Bahia MF,Sarmento B

    更新日期：2012-11-05 00:00:00