Abstract:
OBJECTIVE:The aim of this work was to determine the relevance of Choukroun's platelet-rich fibrin (PRF) in dental implantology by determining the in vitro effects of soluble factors released by PRF clot. We used 3 different cell lines implicated in dental implantology: osteoblast, keratinocyte, and fibroblast. METHODS:Cellular viability, cell proliferation, and gene expression were analyzed using PRF conditioned medium. Three different cells lines were used: SaOS2 (osteoblast), MRC5 (fibroblast), and KB (epithelial cell). RESULTS:The sulforhodamine B assay showed a significant increase in cell number for the undiluted and 1:3 diluted conditioned medium after 24 and 48 hours. There was no effect for the 1:9 dilution. Cell cycle analysis by flow cytometry confirmed the viability test results. After 48 hours, PRF conditioned medium induced gene expression in osteoblasts. Expression of osteopontin and osteocalcin, late osteogenic markers, was observed using reverse transcriptase-polymerase chain reaction (RT-PCR). CONCLUSIONS:This study establishes a model to evaluate, in vitro, the effects of soluble growth factors released by PRF clot. Our work confirmed PRF is useful in stimulating tissue healing and bone regeneration. This work should recommend Choukroun's PRF in numerous implantology clinical applications.
journal_name
Implant Dentjournal_title
Implant dentistryauthors
Clipet F,Tricot S,Alno N,Massot M,Solhi H,Cathelineau G,Perez F,De Mello G,Pellen-Mussi Pdoi
10.1097/ID.0b013e31822b9cb4subject
Has Abstractpub_date
2012-02-01 00:00:00pages
51-6issue
1eissn
1056-6163issn
1538-2982journal_volume
21pub_type
杂志文章abstract:PURPOSE:This review considers possible surgical treatment modalities for induced periimplantitis to regain re-osseointegration as reported in the recent literature. MATERIALS AND METHODS:Electronic searches in MEDLINE/PubMed and Google Scholar databases were performed on experimental studies considering induced periim...
journal_title:Implant dentistry
pub_type: 杂志文章,评审
doi:10.1097/ID.0000000000000712
更新日期:2018-02-01 00:00:00
abstract::We report a case of a postextraction maxillary buccal dehiscence grafted with calcium sulfate to insert a dental implant. The clinical results were supported by histologic analysis of two specimens collected in the healed socket to better understand the biologic effects of calcium sulfate. A 42-year-old white female p...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/01.id.0000156384.05026.a6
更新日期:2005-03-01 00:00:00
abstract:PURPOSE:This study aims to gauge the knowledge and perceptions of undergraduate dental students or residents toward dental implants. MATERIALS AND METHODS:The dental colleges in India were divided into 5 zones, and by random selection method, 7 colleges were selected from each zone. Thus, a total of 35 dental institut...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/ID.0000000000000184
更新日期:2015-04-01 00:00:00
abstract:PURPOSE:To evaluate, in vitro, the leakage observed in internal hexagon and Morse taper implant-abutment connections. MATERIALS:Ten specimens with internal hexagon and 10 with Cone Morse connection were used. The inner parts of 5 implants, per group, were inoculated with Pseudomonas aeruginosa (PS) suspension and 5 im...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/ID.0b013e31825cd472
更新日期:2012-08-01 00:00:00
abstract::Factors affecting cell and tissue responses to dental implant biomaterials are typically characterized as spatial and temporal. Spatially, a dental implant must form an interface with both bone tissue for the development and maintenance of biomechanical stability and soft tissue for the prevention of microbial infecti...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/00008505-199807040-00012
更新日期:1998-01-01 00:00:00
abstract::Accurate knowledge of vital anatomical structures, such as the inferior alveolar nerve, mental nerve, and mental foramen, is critical to achieve favorable results during oral surgical procedures and dental implant placement. Although uncommon, variations in mandibular foramina have been reported and if unnoticed and, ...
journal_title:Implant dentistry
pub_type: 杂志文章,评审
doi:10.1097/ID.0000000000000056
更新日期:2014-04-01 00:00:00
abstract:PURPOSE:The purpose of this study was to compare the bone regenerative capability of using unsintered hydroxyapatite/poly-L-lactic acid (uHA/PLLA) mesh and titanium mesh. MATERIALS AND METHODS:Adult male white rabbits (n = 24) were divided into 2 groups. After incising along the mandible, 3 × 5 mm bone defects were ma...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/ID.0b013e31828336be
更新日期:2013-06-01 00:00:00
abstract::Maxillary alveolar atrophy often limits the placement of dental implants. This article reports on a refinement of a technique for widening the atrophic ridge by splitting the alveolar bone longitudinally. Treatment of a patient with a severely resorbed edentulous maxilla is described. Six 4-mm wide by 13-mm long threa...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/01.id.0000156390.58391.64
更新日期:2005-03-01 00:00:00
abstract:PURPOSE:The aim of this investigation was to assess the efficacy of using subepithelial connective tissue graft (CTG) over the buccal plate in conjunction with 2 different bone graft materials in fresh extraction sites. MATERIALS AND METHODS:Five mongrel dogs were used. Bilateral third (P3) mandibular premolars were e...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/ID.0000000000000307
更新日期:2015-12-01 00:00:00
abstract:BACKGROUND:: A comparison of freeze-dried cancellous and cortical particulate bone allograft was made using a non-human primate model. METHODS AND MATERIALS:: Microparticulate bone allograft (90-300 μm size) made from either cortical or cancellous bone was used to pack standard bone defects in the lower extremities of...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/ID.0b013e3181ad18d6
更新日期:2009-10-01 00:00:00
abstract:PURPOSE:To compare the effectiveness of 2 different techniques to lift the maxillary sinus floor through a crestal approach on fresh human cadaver heads: the Intralift technique using Piezosurgery and the Summers technique using osteotomy. MATERIALS AND METHODS:Two different protocols were simulated on 11 fresh human ...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/ID.0000000000000143
更新日期:2014-10-01 00:00:00
abstract:PURPOSE:The aim of this study was to investigate whether bioactive surfaces were more favorable to bone than bioinert surfaces by evaluating bone responses around two commercial dental implants. MATERIALS AND METHODS:Bioactive fluoride-modified implants (Osseospeed) were compared with bioinert oxidized implants (TiUni...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/ID.0b013e318249f283
更新日期:2012-04-01 00:00:00
abstract:INTRODUCTION:Recognizing the radiographic position of the mental foramina is not sufficient to avoid complications during dental implant surgery. In many cases, the inferior alveolar nerve extends mesially as an "anterior loop" (AL). The aim of this study was to investigate the prevalence and spatial extension of the A...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/ID.0000000000000312
更新日期:2015-10-01 00:00:00
abstract::In 1980 the Veterans Administration Cooperative Studies Program No. 147 initiated a multicenter, multidisciplinary clinical study to evaluate the use of alternative alloys for porcelain-fused-to-metal restorations. An investigation of interexaminer agreement was conducted after seven years utilizing three measures of ...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/00008505-199312000-00004
更新日期:1993-01-01 00:00:00
abstract:PURPOSE:The purpose of this study is the investigation of the application of implant-supported prosthetic restorations using 2 implants supporting a fixed prosthesis during the physical growth and the development period of growing pigs. MATERIALS AND METHODS:This study was carried out on 6 male farm pigs (1 as a contr...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/01.id.0000243323.11666.38
更新日期:2006-12-01 00:00:00
abstract:OBJECTIVES:To evaluate the effectiveness of 4 procedures to disinfect implant surfaces intentionally inoculated with bacteria and afterward to evaluate osteoblast viability to the disinfected implant surfaces. MATERIALS AND METHODS:Eighty-eight commercially pure Osseotite and Nanotite titanium implant discs were inocu...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/ID.0000000000000067
更新日期:2014-08-01 00:00:00
abstract:PURPOSE:The association of doxycycline (DOX) and autogenous bone on repair of critical-size defects was evaluated. MATERIALS AND METHODS:Fifty albino rats were divided into 5 groups (n = 10). A 5-mm diameter defect was treated with: control (CO)-blood clot; DOX in Natrosol (NAT)-10% gel; NAT-gel; particulate autogenou...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/ID.0000000000000783
更新日期:2018-08-01 00:00:00
abstract:PURPOSE:To evaluate the influence of Morse taper implant index on microleakage. MATERIALS AND METHODS:Thirty implants and abutments were divided into 3 groups (n = 10): CM1 (universal post and implant without index), CM2 (universal post and implant with index), and CM3 (abutment and implant with index). To evaluate th...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/ID.0000000000000284
更新日期:2015-10-01 00:00:00
abstract:CASE REPORT:A 46-year-old man with severe hemophilia A, stage A2 HIV infection and chronic hepatitis C genotype 1A, for whom the treatment plan included implant-supported prostheses in 2 mandibular edentulous sections. The protocol followed included factor VIII replacement concentrate and oral antifibrinolytic therapy....
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/ID.0000000000000145
更新日期:2014-10-01 00:00:00
abstract:PURPOSE:To study implant primary stability and bone healing using resonance frequency analysis in different anatomical locations 4 months after placement. MATERIAL AND METHODS:Fifty-six partially edentulous patients restored by dental implants were included. Overall, 214 implants were placed without bone or soft tissu...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/ID.0000000000000019
更新日期:2014-02-01 00:00:00
abstract:PURPOSE:To document the clinical and histologic outcomes of sinus augmentation using a novel allogenic bone substitute as a sole grafting material. MATERIALS:Patients in need of sinus augmentation before implant placement were recruited for this study. Sinus augmentation procedures were performed following a lateral a...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/ID.0b013e3181e59b32
更新日期:2010-08-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to evaluate the outcome of fresh-frozen bone allografts in preprosthetic surgery for implant placement purposes. MATERIALS AND METHODS:The cohort comprised 45 patients treated with fresh-frozen bone block grafts and dental implants. Clinical and radiological evaluations were perf...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/ID.0000000000000412
更新日期:2016-06-01 00:00:00
abstract::The conventional soft tissue conditioning technique around implant demands time and effort for optimizing soft tissue contour. This article describes an alternative technique for achieving proper emergence profile with a custom-milled titanium abutment, interim restoration, and surgical intervention. With this techniq...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/ID.0000000000000339
更新日期:2015-12-01 00:00:00
abstract:PURPOSE:The aim of the present study was to examine the osseointegration in low-density bone tissue for SLAffinity-treated implants with StemBios (SB) cell therapy. MATERIALS AND METHODS:The morphologies of SLAffinity-treated surfaces were characterized using scanning electron microscopy. In the animal model, implants...
journal_title:Implant dentistry
pub_type: 临床试验,杂志文章
doi:10.1097/ID.0000000000000337
更新日期:2016-02-01 00:00:00
abstract::Dental care of the aging patient presents a number of problems not encountered in younger patients. Most of these problems result from tissue changes that occur during aging. Dental implants and implant-retained and/or implant-supported prostheses are viable treatment options for older patients. The majority of elderl...
journal_title:Implant dentistry
pub_type: 杂志文章,评审
doi:10.1097/00008505-199700630-00002
更新日期:1997-10-01 00:00:00
abstract:AIMS:The primary aim of this study was to evaluate the 1-year crestal bone loss and success rate of an immediately placed single-stage implant placed and restored by novice operators. A secondary aim was to determine the patient's assessment of the appearance of the final restoration. METHODS:Fifty-one patients receiv...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/ID.0b013e3181bcd36b
更新日期:2010-02-01 00:00:00
abstract:PURPOSE:The aim of the clinical preliminary trial was to determine the possible relationship among radiologic bone density, primary implant stability, and tactile sense measurements, and to clarify their possible association with histological measurements at endosseous implant osteotomy sites. MATERIALS:Eleven mandibu...
journal_title:Implant dentistry
pub_type: 临床试验,杂志文章
doi:10.1097/ID.0b013e31819ecc12
更新日期:2009-08-01 00:00:00
abstract:BACKGROUND:The aim was to identify and evaluate those surgical protocols reporting on positive clinical outcomes for treating peri-implantitis with 12 or more months of follow-up. Method of surface decontamination (SDC) was evaluated for any correlation with outcomes. RESULTS:A literature search was performed of all a...
journal_title:Implant dentistry
pub_type: 杂志文章,评审
doi:10.1097/ID.0000000000000428
更新日期:2016-06-01 00:00:00
abstract:PURPOSE:To present a vertical ridge augmentation with a composite synthetic bone graft by the mixed bone-filling materials and autogenous bone chips retrieved from the implant osteotomy site for a case for esthetic implant prosthesis on the atrophied alveolar bone. MATERIALS:Nonresorbable and resorbable hydroxyapatite...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/ID.0b013e3181fce1a9
更新日期:2010-12-01 00:00:00
abstract::The biologic principle of guided bone regeneration has been successfully used to prevent bone loss in extraction sites. This study comprises 420 cases of alveolar ridge maintenance in preparation for dental implant placements. Nonexpanded polytetrafluoroethylene membranes were positioned over all extraction sites and ...
journal_title:Implant dentistry
pub_type: 杂志文章
doi:10.1097/ID.0b013e3181cda72c
更新日期:2010-02-01 00:00:00