Abstract：
:The systemic pharmacokinetics and pharmacodynamics of small molecules are determined by subcellular transport phenomena. Although approaches used to study the subcellular distribution of small molecules have gradually evolved over the past several decades, experimental analysis and prediction of cellular pharmacokinetics remains a challenge. In this review, we survey the progress of subcellular distribution research since the 1960s, with a focus on the advantages, disadvantages and limitations of the various experimental techniques. Critical review of the existing body of knowledge points to many opportunities to advance the rational design of organelle-targeted chemical agents. These opportunities include (1) development of quantitative, non-fluorescence-based, whole cell methods and techniques to measure the subcellular distribution of chemical agents in multiple compartments; (2) exploratory experimentation with nonspecific transport probes that have not been enriched with putative, organelle-targeting features; (3) elaboration of hypothesis-driven, mechanistic and modeling-based approaches to guide experiments aimed at elucidating subcellular distribution and transport; and (4) introduction of revolutionary conceptual approaches borrowed from the field of synthetic biology combined with cutting edge experimental strategies. In our laboratory, state-of-the-art subcellular transport studies are now being aimed at understanding the formation of new intracellular membrane structures in response to drug therapy, exploring the function of drug-membrane complexes as intracellular drug depots, and synthesizing new organelles with extraordinary physical and chemical properties.
journal_name
Mol Pharmjournal_title
Molecular pharmaceuticsauthors
Zheng N,Tsai HN,Zhang X,Rosania GRdoi
10.1021/mp200092vsubject
Has Abstractpub_date
2011-10-03 00:00:00pages
1619-28issue
5eissn
1543-8384issn
1543-8392journal_volume
8pub_type
杂志文章,评审abstract：:As a chronic inflammatory and angiogenic disease with increased morbidity and mortality, rheumatoid arthritis (RA) is characterized by the proliferation of synovial tissue and the accumulation of excessive mononuclear infiltration, which always results in the joint deformity, disability, and eventually the destruction...
journal_title：Molecular pharmaceutics
pub_type： 杂志文章
doi：10.1021/acs.molpharmaceut.7b00505
更新日期：2017-09-05 00:00:00
abstract：:PLGA was functionalized with PEG and biotin using click chemistry to generate a biotin receptor targeted copolymer (biotinylated-PEG-PLGA) which in turn was used to fabricate ultrafine nanoparticles (BPNP) of doxorubicin hydrochloride (DOX) for effective delivery in 4T1 cell induced breast cancer. However, adequate en...
journal_title：Molecular pharmaceutics
pub_type： 杂志文章
doi：10.1021/acs.molpharmaceut.7b00310
更新日期：2017-08-07 00:00:00
abstract：:Olive pollen is one of the most important causes of respiratory allergy, with Ole e 1 being the most clinically relevant sensitizing allergen. Peptide-based vaccines represent promising therapeutic approaches, but the use of adjuvants is required to strengthen the weak immunogenicity of small peptides. We propose the ...
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pub_type： 杂志文章
doi：10.1021/acs.molpharmaceut.9b01082
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pub_type： 杂志文章
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更新日期：2007-03-01 00:00:00
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pub_type： 杂志文章
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更新日期：2016-03-07 00:00:00
abstract：:In humans, C-X-C chemokine receptor type 4 (CXCR4) is a protein that is encoded by the CXCR4 gene and binds the ligand CXCL12 (also known as SDF-1). The CXCR4-CXCL12 interaction in cancer elicits biological activities that result in tumor progression and has accordingly been the subject of significant investigation fo...
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更新日期：2013-11-04 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1021/mp2001654
更新日期：2011-10-03 00:00:00
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更新日期：2019-06-03 00:00:00
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doi：10.1021/acs.molpharmaceut.9b00894
更新日期：2020-04-06 00:00:00
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pub_type： 杂志文章
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更新日期：2019-01-07 00:00:00
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更新日期：2018-09-04 00:00:00
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pub_type： 杂志文章
doi：10.1021/acs.molpharmaceut.5b00089
更新日期：2015-09-08 00:00:00
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pub_type： 杂志文章
doi：10.1021/acs.molpharmaceut.0c00492
更新日期：2020-08-03 00:00:00
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journal_title：Molecular pharmaceutics
pub_type： 杂志文章
doi：10.1021/mp3003336
更新日期：2012-10-01 00:00:00
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journal_title：Molecular pharmaceutics
pub_type： 杂志文章
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更新日期：2014-10-06 00:00:00
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更新日期：2019-08-05 00:00:00
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更新日期：2013-01-07 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2010-12-06 00:00:00
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更新日期：2012-04-02 00:00:00
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更新日期：2020-07-06 00:00:00
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pub_type： 杂志文章
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更新日期：2012-05-07 00:00:00
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更新日期：2019-02-04 00:00:00
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journal_title：Molecular pharmaceutics
pub_type： 杂志文章
doi：10.1021/acs.molpharmaceut.6b00438
更新日期：2016-09-06 00:00:00
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doi：10.1021/acs.molpharmaceut.8b00424
更新日期：2018-08-06 00:00:00
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journal_title：Molecular pharmaceutics
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doi：10.1021/mp3006209
更新日期：2013-06-03 00:00:00
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更新日期：2017-10-02 00:00:00
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journal_title：Molecular pharmaceutics
pub_type： 杂志文章
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更新日期：2018-08-06 00:00:00
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journal_title：Molecular pharmaceutics
pub_type： 杂志文章
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更新日期：2016-08-01 00:00:00
