In vivo treatment with progestogens causes immunosuppression of carp Cyprinus carpio leucocytes by affecting nitric oxide production and arginase activity.

Abstract:

:In this study, carp Cyprinus carpio were injected with various steroid compounds, including synthetic and natural progestogens and the glucocorticoid cortisol, to investigate effects on leucocytes isolated from their kidneys. Injection of cortisol led to an increased spleeno-somatic index (I(S)) on day 21 post-injection (pi) and immunosuppressive effects measured as decreased nitric oxide (NO) production and increased arginase activity in isolated leucocytes on days 14 and 21 pi, respectively. Moreover, reduced NO production was also observed after injection of the synthetic progestogens, levonorgestrel (LEV) and medroxyprogesterone acetate. In addition, LEV influenced arginase activity in head kidney cells on day 14 and day 21 pi. This study is the first demonstration in fishes that the application of these steroid compounds in vivo affects NO production and arginase activity of isolated leucocytes.

journal_name

J Fish Biol

journal_title

Journal of fish biology

authors

Pietsch C,Neumann N,Preuer T,Kloas W

doi

10.1111/j.1095-8649.2011.02981.x

subject

Has Abstract

pub_date

2011-07-01 00:00:00

pages

53-69

issue

1

eissn

0022-1112

issn

1095-8649

journal_volume

79

pub_type

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