Abstract:
BACKGROUND:Better understanding of the pathophysiological mechanisms involved in severe dysmotility disorders is crucial to improve patient management and identify novel therapeutic targets. Recent studies suggested that endoscopic full-thickness biopsies (eFTBs) could be developed as an alternative to surgical biopsies. However, currently it remains unknown whether eFTBs would allow myenteric plexus analysis on whole mounts and the evaluation of neuromuscular transmission. OBJECTIVE:To determine with eFTB specimens the ability to analyze on whole mounts the key parameters of the myenteric plexus, ie, ganglia and neurons, and to perform functional evaluation of neuromuscular transmission. DESIGN:An experimental pilot study in 6 pigs was conducted in accordance with French institutional guidelines. INTERVENTION:Under general anesthesia, pigs underwent a rectosigmoidoscopy. In each pig, an eFTB was performed at 25, 30, and 35 cm from the anal margin with an EMR-based technique. Tissue specimens were immediately processed for immunohistochemical and/or functional ex vivo analysis of neuromuscular transmission. In 2 pigs, over-the-scope clips were used to seal the perforation. MAIN OUTCOME MEASUREMENTS:Feasibility of obtaining specimens containing myenteric plexus and muscularis propria, quantitative and standardized immunohistochemical evaluation of ganglia and myenteric neurons, ex vivo assessment of neuromuscular transmission and its pharmacology, and closure rate (ancillary study). RESULTS:Adequate tissue specimens were obtained in 100% of the procedures, on average, in 6±2 minutes. Immunohistochemical analysis of a whole mount of the myenteric plexus showed that each eFTB contained 14±5 ganglia and 1562±1066 myenteric neurons. In circular muscle strips, electrical field stimulation or exposure to a pharmacological agent induced a specific tissue response. A successful closure was achieved in 50% of cases. LIMITATIONS:Nonsurvival study; safety of the procedure needs to be specifically assessed and compared with recently published data. CONCLUSIONS:We demonstrate, for the first time, that full-thickness biopsy specimens obtained by using an endoscopic approach allow the performance of a precise study of the ENS phenotype on whole mounts of the myenteric plexus and the performance of functional studies such as evaluation of neuromuscular transmission. However, further studies are warranted to identify the optimal and safest endoscopic procedure before application of eFTB in humans.
journal_name
Gastrointest Endoscjournal_title
Gastrointestinal endoscopyauthors
Neunlist M,Coquenlorge S,Aubert P,Duchalais-Dassonneville E,des Varannes SB,Meurette G,Coron Edoi
10.1016/j.gie.2011.01.041subject
Has Abstractpub_date
2011-05-01 00:00:00pages
1029-34issue
5eissn
0016-5107issn
1097-6779pii
S0016-5107(11)00057-5journal_volume
73pub_type
杂志文章abstract:OBJECTIVE:To determine the influence of biliary drainage catheter placement on bile duct wall thickness, we performed intraductal ultrasonography (IDUS) in patients before and after biliary drainage. METHODS:Patients underwent IDUS before and after either short-term (n = 9, 6 to 8 days) or long-term (n = 9, 14 to 35 d...
journal_title:Gastrointestinal endoscopy
pub_type: 临床试验,杂志文章
doi:10.1016/s0016-5107(98)70295-0
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND:New therapies are needed for pancreatic cancer. OBJECTIVE:To determine the feasibility and safety of a new endoscopic treatment. Secondary endpoints were to determine effects on tumor growth measured with CT scan and to find the overall survival. DESIGN:A cohort study of patients with local progression of ...
journal_title:Gastrointestinal endoscopy
pub_type: 临床试验,杂志文章
doi:10.1016/j.gie.2012.08.006
更新日期:2012-12-01 00:00:00
abstract::We investigated regional variation in resting focal gastric mucosal blood flow obtained by endoscopic laser-Doppler flowmetry in patients with symptoms of dyspepsia or peptic ulcer (N = 12) and asymptomatic healthy volunteers (N = 6). Mucosal blood flow was measured for 1 min at the duodenum (first part), prepylorus, ...
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abstract:BACKGROUND:The Rockall scoring system was developed in unselected patients, the majority of whom did not receive endoscopic therapy. The aim of this study was to assess the validity of the Rockall system in high-risk patients who undergo endoscopic therapy for peptic ulcer hemorrhage. METHODS:Rockall scores were calcu...
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abstract:BACKGROUND AND AIMS:Unresectable malignant biliary strictures are generally managed by palliative stent placement for drainage of biliary tree. Recently, radiofrequency ablation (RFA) has been used to improve the patency of biliary stents in these patients. Several studies have evaluated the effectiveness of biliary st...
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abstract::The endoscopic Congo red test allows accurate and rapid evaluation of the completeness of vagotomy and may result in a lower incidence of postoperative incomplete vagotomy. This report describes 44 patients tested during proximal gastric vagotomy. Evidence of incomplete vagotomy was found in over 95% at the conclusion...
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pub_type: 临床试验,杂志文章
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更新日期:2016-10-01 00:00:00
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更新日期:2017-04-01 00:00:00
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journal_title:Gastrointestinal endoscopy
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更新日期:2004-02-01 00:00:00
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doi:10.1016/s0016-5107(03)02304-6
更新日期:2003-12-01 00:00:00
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doi:10.1016/j.gie.2009.09.043
更新日期:2010-03-01 00:00:00
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doi:10.1016/s0016-5107(02)70181-8
更新日期:2002-08-01 00:00:00
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更新日期:2019-05-01 00:00:00
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journal_title:Gastrointestinal endoscopy
pub_type: 杂志文章,评审
doi:10.1016/j.gie.2007.06.001
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abstract:BACKGROUND:Endoscopic resection of large colorectal lesions is associated with high complication rates. OBJECTIVE:To evaluate the effect of prophylactic clip closure of polypectomy sites after resection of large (≥2 cm) sessile and flat colorectal lesions. DESIGN:Retrospective study. SETTING:Tertiary referral center...
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pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2002-01-01 00:00:00
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journal_title:Gastrointestinal endoscopy
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更新日期:2006-06-01 00:00:00
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journal_title:Gastrointestinal endoscopy
pub_type: 杂志文章,meta分析,评审
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更新日期:2014-02-01 00:00:00
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journal_title:Gastrointestinal endoscopy
pub_type: 杂志文章,评审
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更新日期:2021-02-01 00:00:00
abstract:BACKGROUND:The ability to access the abdominal cavity though a direct (modified-PEG type) gastric incision to perform natural orifice transluminal endoscopic surgery (NOTES) has been demonstrated in the literature. However, the optimal technique to access the abdomen remains unknown. OBJECTIVE:The aim of this study wa...
journal_title:Gastrointestinal endoscopy
pub_type: 杂志文章
doi:10.1016/j.gie.2007.09.023
更新日期:2008-04-01 00:00:00
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journal_title:Gastrointestinal endoscopy
pub_type: 杂志文章
doi:10.1016/j.gie.2008.07.025
更新日期:2009-05-01 00:00:00
abstract:BACKGROUND:The aim of this study was to evaluate the effectiveness of endoscopic sphincterotomy for preoperative and postoperative complications of hepatic hydatid disease. METHODS:Nineteen patients underwent endoscopic treatment for complications of hepatic hydatid disease. Indications for ERCP in 5 patients treated ...
journal_title:Gastrointestinal endoscopy
pub_type: 杂志文章
doi:10.1067/mge.2001.118256
更新日期:2001-10-01 00:00:00
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journal_title:Gastrointestinal endoscopy
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
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更新日期:2003-11-01 00:00:00
abstract:BACKGROUND:The use of disposable biopsy forceps seems to be effective and safer than reusable biopsy forceps with respect to the risk of infection transmission. The results of cost analysis studies comparing reusable versus disposable biopsy forceps are conflicting. This study compared the cost of reusable versus dispo...
journal_title:Gastrointestinal endoscopy
pub_type: 杂志文章
doi:10.1067/mge.2003.341
更新日期:2003-08-01 00:00:00
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journal_title:Gastrointestinal endoscopy
pub_type: 杂志文章
doi:10.1016/j.gie.2020.05.065
更新日期:2021-02-01 00:00:00