Abstract:
:Map based cloning in Arabidopsis thaliana can be a difficult and time-consuming process, specifically if the phenotype is subtle and scoring labour intensive. Here, we have re-sequenced the 120-Mb genome of a novel Arabidopsis clock mutant early bird (ebi-1) in Wassilewskija (Ws-2). We demonstrate the utility of sequencing a backcrossed line in limiting the number of SNPs considered. We identify a SNP in the gene AtNFXL-2 as the likely cause of the ebi-1 phenotype.
journal_name
Genome Bioljournal_title
Genome biologyauthors
Ashelford K,Eriksson ME,Allen CM,D'Amore R,Johansson M,Gould P,Kay S,Millar AJ,Hall N,Hall Adoi
10.1186/gb-2011-12-3-r28subject
Has Abstractpub_date
2011-01-01 00:00:00pages
R28issue
3eissn
1474-7596issn
1474-760Xpii
gb-2011-12-3-r28journal_volume
12pub_type
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