Abstract:
:A fundamental step in the evolution of the visual system is the gene duplication of visual opsins and differentiation between the duplicates in absorption spectra and expression pattern in the retina. However, our understanding of the mechanism of expression differentiation is far behind that of spectral tuning of opsins. Zebrafish (Danio rerio) have two red-sensitive cone opsin genes, LWS-1 and LWS-2. These genes are arrayed in a tail-to-head manner, in this order, and are both expressed in the long member of double cones (LDCs) in the retina. Expression of the longer-wave sensitive LWS-1 occurs later in development and is thus confined to the peripheral, especially ventral-nasal region of the adult retina, whereas expression of LWS-2 occurs earlier and is confined to the central region of the adult retina, shifted slightly to the dorsal-temporal region. In this study, we employed a transgenic reporter assay using fluorescent proteins and P1-artificial chromosome (PAC) clones encompassing the two genes and identified a 0.6-kb "LWS-activating region" (LAR) upstream of LWS-1, which regulates expression of both genes. Under the 2.6-kb flanking upstream region containing the LAR, the expression pattern of LWS-1 was recapitulated by the fluorescent reporter. On the other hand, when LAR was directly conjugated to the LWS-2 upstream region, the reporter was expressed in the LDCs but also across the entire outer nuclear layer. Deletion of LAR from the PAC clones drastically lowered the reporter expression of the two genes. These results suggest that LAR regulates both LWS-1 and LWS-2 by enhancing their expression and that interaction of LAR with the promoters is competitive between the two genes in a developmentally restricted manner. Sharing a regulatory region between duplicated genes could be a general way to facilitate the expression differentiation in duplicated visual opsins.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Tsujimura T,Hosoya T,Kawamura Sdoi
10.1371/journal.pgen.1001245subject
Has Abstractpub_date
2010-12-16 00:00:00pages
e1001245issue
12eissn
1553-7390issn
1553-7404journal_volume
6pub_type
杂志文章相关文献
PLoS Genetics文献大全abstract::Mollicutes is a class of parasitic bacteria that have evolved from a common Firmicutes ancestor mostly by massive genome reduction. With genomes under 1 Mbp in size, most Mollicutes species retain the capacity to replicate and grow autonomously. The major goal of this work was to identify the minimal set of proteins t...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004363
更新日期:2014-05-08 00:00:00
abstract::The heterochromatin-enriched HP1 proteins play a critical role in regulation of transcription. These proteins contain two related domains known as the chromo- and the chromoshadow-domain. The chromo-domain binds histone H3 tails methylated on lysine 9. However, in vivo and in vitro experiments have shown that the affi...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000769
更新日期:2009-12-01 00:00:00
abstract::Skin pigment patterns are important, being under strong selection for multiple roles including camouflage and UV protection. Pigment cells underlying these patterns form from adult pigment stem cells (APSCs). In zebrafish, APSCs derive from embryonic neural crest cells, but sit dormant until activated to produce pigme...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007941
更新日期:2019-02-27 00:00:00
abstract::We propose a novel statistical framework for integrating the result from molecular quantitative trait loci (QTL) mapping into genome-wide genetic association analysis of complex traits, with the primary objectives of quantitatively assessing the enrichment of the molecular QTLs in complex trait-associated genetic vari...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006646
更新日期:2017-03-09 00:00:00
abstract::Although autism has a clear genetic component, the high genetic heterogeneity of the disorder has been a challenge for the identification of causative genes. We used homozygosity analysis to identify probands from nonconsanguineous families that showed evidence of distant shared ancestry, suggesting potentially recess...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002635
更新日期:2012-01-01 00:00:00
abstract::For many decades, Salmonella enterica has been subdivided by serological properties into serovars or further subdivided for epidemiological tracing by a variety of diagnostic tests with higher resolution. Recently, it has been proposed that so-called eBurst groups (eBGs) based on the alleles of seven housekeeping gene...
journal_title:PLoS genetics
pub_type: 杂志文章,评审
doi:10.1371/journal.pgen.1007261
更新日期:2018-04-05 00:00:00
abstract::Genome-wide association studies have successfully identified thousands of loci for a range of human complex traits and diseases. The proportion of phenotypic variance explained by significant associations is, however, limited. Given the same dense SNP panels, mixed model analyses capture a greater proportion of phenot...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005804
更新日期:2016-02-02 00:00:00
abstract::Recent comparative genomic analysis of alternative splicing has shown that protein modularity is an important criterion for functional alternative splicing events. Exons that are alternatively spliced in multiple organisms are much more likely to be an exact multiple of 3 nt in length, representing a class of "modular...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.0010034
更新日期:2005-09-01 00:00:00
abstract::In humans, the absence or irreversible loss of hair cells, the sensory mechanoreceptors in the cochlea, accounts for a large majority of acquired and congenital hearing disorders. In the auditory and vestibular neuroepithelia of the inner ear, hair cells are accompanied by another cell type called supporting cells. Th...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000455
更新日期:2009-04-01 00:00:00
abstract::In understanding the etiology of breast cancer, the contributions of both genetic and environmental risk factors are further complicated by the impact of breast developmental stage. Specifically, the time period ranging from childhood to young adulthood represents a critical developmental window in a woman's life when...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006261
更新日期:2016-08-18 00:00:00
abstract::The lipopolysaccharide O-antigen structure expressed by the European Helicobacter pylori model strain G27 encompasses a trisaccharide, an intervening glucan-heptan and distal Lewis antigens that promote immune escape. However, several gaps still remain in the corresponding biosynthetic pathway. Here, systematic mutage...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008497
更新日期:2019-11-20 00:00:00
abstract::The phenotype of the spontaneous mutant mouse hop-sterile (hop) is characterized by a hopping gait, polydactyly, hydrocephalus, and male sterility. Previous analyses of the hop mouse revealed a deficiency of inner dynein arms in motile cilia and a lack of sperm flagella, potentially accounting for the hydrocephalus an...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004689
更新日期:2014-10-23 00:00:00
abstract::Human genome-wide association studies have linked single nucleotide polymorphisms (SNPs) on chromosome 9p21.3 near the INK4/ARF (CDKN2a/b) locus with susceptibility to atherosclerotic vascular disease (ASVD). Although this locus encodes three well-characterized tumor suppressors, p16(INK4a), p15(INK4b), and ARF, the S...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1001233
更新日期:2010-12-02 00:00:00
abstract::Hematopoietic stem cells (HSCs) are rare quiescent cells that continuously replenish the cellular components of the peripheral blood. Observing that the ataxia-associated gene Ataxin-1-like (Atxn1L) was highly expressed in HSCs, we examined its role in HSC function through in vitro and in vivo assays. Mice lacking Atx...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003359
更新日期:2013-03-01 00:00:00
abstract::UV-induced DNA damage causes repression of RNA synthesis. Following the removal of DNA lesions, transcription recovery operates through a process that is not understood yet. Here we show that knocking-out of the histone methyltransferase DOT1L in mouse embryonic fibroblasts (MEF(DOT1L)) leads to a UV hypersensitivity ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003611
更新日期:2013-01-01 00:00:00
abstract::Recent genome-wide meta-analyses identified 157 loci associated with cross-sectional lipid traits. Here we tested whether these loci associate (singly and in trait-specific genetic risk scores [GRS]) with longitudinal changes in total cholesterol (TC) and triglyceride (TG) levels in a population-based prospective coho...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004388
更新日期:2014-06-12 00:00:00
abstract::[This corrects the article DOI: 10.1371/journal.pgen.1006940.]. ...
journal_title:PLoS genetics
pub_type: 已发布勘误
doi:10.1371/journal.pgen.1007035
更新日期:2017-10-03 00:00:00
abstract::Replication Protein A (RPA) is a heterotrimeric, single-stranded DNA (ssDNA)-binding complex required for DNA replication and repair, homologous recombination, DNA damage checkpoint signaling, and telomere maintenance. Whilst the larger RPA subunits, Rpa1 and Rpa2, have essential interactions with ssDNA, the molecular...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1001138
更新日期:2010-09-23 00:00:00
abstract::Replication forks stall at different DNA obstacles such as those originated by transcription. Fork stalling can lead to DNA double-strand breaks (DSBs) that will be preferentially repaired by homologous recombination when the sister chromatid is available. The Rrm3 helicase is a replisome component that promotes repli...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006781
更新日期:2017-05-05 00:00:00
abstract::Pervasive natural selection can strongly influence observed patterns of genetic variation, but these effects remain poorly understood when multiple selected variants segregate in nearby regions of the genome. Classical population genetics fails to account for interference between linked mutations, which grows increasi...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004222
更新日期:2014-03-27 00:00:00
abstract::North African populations are distinct from sub-Saharan Africans based on cultural, linguistic, and phenotypic attributes; however, the time and the extent of genetic divergence between populations north and south of the Sahara remain poorly understood. Here, we interrogate the multilayered history of North Africa by ...
journal_title:PLoS genetics
pub_type: 历史文章,杂志文章
doi:10.1371/journal.pgen.1002397
更新日期:2012-01-01 00:00:00
abstract::Translesion DNA synthesis (TLS) is a DNA damage tolerance mechanism in which specialized low-fidelity DNA polymerases bypass replication-blocking lesions, and it is usually associated with mutagenesis. In Saccharomyces cerevisiae a key event in TLS is the monoubiquitination of PCNA, which enables recruitment of the sp...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002262
更新日期:2011-09-01 00:00:00
abstract::During transcription, the nascent pre-mRNA undergoes a series of processing steps before being exported to the cytoplasm. The 3'-end processing machinery involves different proteins, this function being crucial to cell growth and viability in eukaryotes. Here, we found that the rna14-1, rna15-1, and hrp1-5 alleles of ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004203
更新日期:2014-03-06 00:00:00
abstract::[This corrects the article DOI: 10.1371/journal.pgen.1006396.]. ...
journal_title:PLoS genetics
pub_type: 杂志文章,已发布勘误
doi:10.1371/journal.pgen.1007639
更新日期:2018-08-30 00:00:00
abstract::Inter-individual variation in gene regulatory elements is hypothesized to play a causative role in adverse drug reactions and reduced drug activity. However, relatively little is known about the location and function of drug-dependent elements. To uncover drug-associated elements in a genome-wide manner, we performed ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004648
更新日期:2014-10-02 00:00:00
abstract::Anti-tumor necrosis factor alpha (anti-TNF) biologic therapy is a widely used treatment for rheumatoid arthritis (RA). It is unknown why some RA patients fail to respond adequately to anti-TNF therapy, which limits the development of clinical biomarkers to predict response or new drugs to target refractory cases. To u...
journal_title:PLoS genetics
pub_type: 杂志文章,meta分析
doi:10.1371/journal.pgen.1003394
更新日期:2013-03-01 00:00:00
abstract::Detailed information about stage-specific changes in gene expression is crucial for the understanding of the gene regulatory networks underlying development. Here, we describe the global gene expression dynamics during early flower development, a key process in the life cycle of a plant, during which floral patterning...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.0020117.eor
更新日期:2006-07-01 00:00:00
abstract::TGFβs act through canonical and non-canonical pathways, and canonical signals are transduced via Smad2 and Smad3. However, the contribution of canonical vs. non-canonical pathways in cartilage is unknown because the role of Smad2 in chondrogenesis has not been investigated in vivo. Therefore, we analyzed mice in which...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006352
更新日期:2016-10-14 00:00:00
abstract::The differentiation of cells into distinct cell types, each of which is heritable for many generations, underlies many biological phenomena. White and opaque cells of the fungal pathogen Candida albicans are two such heritable cell types, each thought to be adapted to unique niches within their human host. To systemat...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1001070
更新日期:2010-08-19 00:00:00
abstract::The molecular genetic mechanisms of sex determination are not known for most vertebrates, including zebrafish. We identified a mutation in the zebrafish fancl gene that causes homozygous mutants to develop as fertile males due to female-to-male sex reversal. Fancl is a member of the Fanconi Anemia/BRCA DNA repair path...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1001034
更新日期:2010-07-22 00:00:00