Abstract:
:Replication forks stall at different DNA obstacles such as those originated by transcription. Fork stalling can lead to DNA double-strand breaks (DSBs) that will be preferentially repaired by homologous recombination when the sister chromatid is available. The Rrm3 helicase is a replisome component that promotes replication upon fork stalling, accumulates at highly transcribed regions and prevents not only transcription-induced replication fork stalling but also transcription-associated hyper-recombination. This led us to explore the possible role of Rrm3 in the repair of DSBs when originating at the passage of the replication fork. Using a mini-HO system that induces mainly single-stranded DNA breaks, we show that rrm3Δ cells are defective in DSB repair. The defect is clearly seen in sister chromatid recombination, the major repair pathway of replication-born DSBs. Our results indicate that Rrm3 recruitment to replication-born DSBs is crucial for viability, uncovering a new role for Rrm3 in the repair of broken replication forks.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Muñoz-Galván S,García-Rubio M,Ortega P,Ruiz JF,Jimeno S,Pardo B,Gómez-González B,Aguilera Adoi
10.1371/journal.pgen.1006781subject
Has Abstractpub_date
2017-05-05 00:00:00pages
e1006781issue
5eissn
1553-7390issn
1553-7404pii
PGENETICS-D-16-02657journal_volume
13pub_type
杂志文章相关文献
PLoS Genetics文献大全abstract::Cellulolytic fungi have evolved a complex regulatory network to maintain the precise balance of nutrients required for growth and hydrolytic enzyme production. When fungi are exposed to cellulose, the transcript levels of cellulase genes rapidly increase and then decline. However, the mechanisms underlying this bell-s...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008510
更新日期:2019-11-25 00:00:00
abstract::Wnt signaling provides a paradigm for cell-cell signals that regulate embryonic development and stem cell homeostasis and are inappropriately activated in cancers. The tumor suppressors APC and Axin form the core of the multiprotein destruction complex, which targets the Wnt-effector beta-catenin for phosphorylation, ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007339
更新日期:2018-04-11 00:00:00
abstract::[This corrects the article DOI: 10.1371/journal.pgen.1005854.]. ...
journal_title:PLoS genetics
pub_type: 已发布勘误
doi:10.1371/journal.pgen.1006064
更新日期:2016-05-12 00:00:00
abstract::Shifts between epigenetic states of transcriptional activity are typically correlated with changes in epigenetic marks. However, exceptions to this rule suggest the existence of additional, as yet uncharacterized, layers of epigenetic regulation. MOM1, a protein of 2,001 amino acids that acts as a transcriptional sile...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002484
更新日期:2012-02-01 00:00:00
abstract::We performed a genome-scale chromatin immunoprecipitation (ChIP)-chip comparison of two modifications (trimethylation of lysine 9 [H3me3K9] and trimethylation of lysine 27 [H3me3K27]) of histone H3 in Ntera2 testicular carcinoma cells and in three different anatomical sources of primary human fibroblasts. We found tha...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.0030089
更新日期:2007-06-01 00:00:00
abstract::Caenorhabditis elegans SKN-1 (ortholog of mammalian Nrf1/2/3) is critical for oxidative stress resistance and promotes longevity under reduced insulin/IGF-1-like signaling (IIS), dietary restriction (DR), and normal conditions. SKN-1 inducibly activates genes involved in detoxification, protein homeostasis, and other ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1001048
更新日期:2010-08-05 00:00:00
abstract::DNA methyltransferases are ubiquitous enzymes conserved in bacteria, plants and opisthokonta. These enzymes, which methylate cytosines, are involved in numerous biological processes, notably development. In mammals and higher plants, methylation patterns established and maintained by the cytosine DNA methyltransferase...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008086
更新日期:2019-08-14 00:00:00
abstract::Genetic background exerts a strong modulatory effect on the toxicity of aggregation-prone proteins in conformational diseases. In addition to influencing the misfolding and aggregation behavior of the mutant proteins, polymorphisms in putative modifier genes may affect the molecular processes leading to the disease ph...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000399
更新日期:2009-03-01 00:00:00
abstract::TGFβs act through canonical and non-canonical pathways, and canonical signals are transduced via Smad2 and Smad3. However, the contribution of canonical vs. non-canonical pathways in cartilage is unknown because the role of Smad2 in chondrogenesis has not been investigated in vivo. Therefore, we analyzed mice in which...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006352
更新日期:2016-10-14 00:00:00
abstract::We resequenced and phased 27 kb of DNA within 580 kb of the MHC class II region in 158 population chromosomes, most of which were conserved extended haplotypes (CEHs) of European descent or contained their centromeric fragments. We determined the single nucleotide polymorphism and deletion-insertion polymorphism allel...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004637
更新日期:2014-10-09 00:00:00
abstract::Autosomal recessive congenital ichthyosis (ARCI) is a rare genetic disorder of the skin characterized by abnormal desquamation over the whole body. In this study we report four patients from three consanguineous Tunisian families with skin, eye, heart, and skeletal anomalies, who harbor a homozygous contiguous gene de...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003536
更新日期:2013-06-01 00:00:00
abstract::Numerous instances of presence/absence variations for introns have been documented in eukaryotes, and some cases of recurrent loss of the same intron have been suggested. However, there has been no comprehensive or phylogenetically deep analysis of recurrent intron loss. Of 883 cases of intron presence/absence variati...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004843
更新日期:2014-12-04 00:00:00
abstract::Human cancer is caused by the interplay of mutations in oncogenes and tumor suppressor genes and inherited variations in cancer susceptibility genes. While many of the tumor initiating mutations are well characterized, the effect of genetic background variation on disease onset and progression is less understood. We h...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005236
更新日期:2015-05-15 00:00:00
abstract::There is growing evidence that gene expression profiling of peripheral blood cells is a valuable tool for assessing gene signatures related to exposure, drug-response, or disease. However, the true promise of this approach can not be estimated until the scientific community has robust baseline data describing variatio...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000873
更新日期:2010-03-12 00:00:00
abstract::Although endocrine changes are known to modulate the timing of major developmental transitions, the genetic mechanisms underlying these changes remain poorly understood. In insects, two developmental hormones, juvenile hormone (JH) and ecdysteroids, are coordinated with each other to induce developmental changes assoc...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004425
更新日期:2014-06-19 00:00:00
abstract::We recently showed that cassette integration and deletion in integron platforms were occurring through unconventional site-specific recombination reactions involving only the bottom strand of attC sites. The lack of sequence conservation among attC sites led us to hypothesize that sequence-independent structural recog...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000632
更新日期:2009-09-01 00:00:00
abstract::miRNAs are small RNAs directing many developmental processes by posttranscriptional regulation of protein-coding genes. We uncovered a new role for miR-1-1/133a-2 and miR-1-2/133a-1 clusters in the specification of embryonic cardiomyocytes allowing transition from an immature state characterized by expression of smoot...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003793
更新日期:2013-01-01 00:00:00
abstract::The functional contribution of CNV to human biology and disease pathophysiology has undergone limited exploration. Recent observations in humans indicate a tentative link between CNV and weight regulation. Smith-Magenis syndrome (SMS), manifesting obesity and hypercholesterolemia, results from a deletion CNV at 17p11....
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002713
更新日期:2012-01-01 00:00:00
abstract::Variably expressive copy-number variants (CNVs) are characterized by extensive phenotypic heterogeneity of neuropsychiatric phenotypes. Approaches to identify single causative genes for these phenotypes within each CNV have not been successful. Here, we posit using multiple lines of evidence, including pathogenicity m...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007879
更新日期:2019-01-17 00:00:00
abstract::Magnetosomes of magnetotactic bacteria contain well-ordered nanocrystals for magnetic navigation and have recently emerged as the most sophisticated model system to study the formation of membrane bounded organelles in prokaryotes. Magnetosome biosynthesis is thought to begin with the formation of a dedicated compartm...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006101
更新日期:2016-06-10 00:00:00
abstract::Polycomb proteins are epigenetic regulators that localize to developmental loci in the early embryo where they mediate lineage-specific gene repression. In Drosophila, these repressors are recruited to sequence elements by DNA binding proteins associated with Polycomb repressive complex 2 (PRC2). However, the sequence...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1001244
更新日期:2010-12-09 00:00:00
abstract::Organisms perceive changes in their dietary environment and enact a suite of behavioral and metabolic adaptations that can impact motivational behavior, disease resistance, and longevity. However, the precise nature and mechanism of these dietary responses is not known. We have uncovered a novel link between dietary f...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002668
更新日期:2012-01-01 00:00:00
abstract::Immunoregulatory cytokine interleukin-10 (IL-10) is elevated in sera from patients with systemic lupus erythematosus (SLE) correlating with disease activity. The established association of IL10 with SLE and other autoimmune diseases led us to fine map causal variant(s) and to explore underlying mechanisms. We assessed...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003870
更新日期:2013-01-01 00:00:00
abstract::A variety of human diseases arise from mutations that alter muscle contraction. Evolutionary conservation allows genetic studies in Drosophila melanogaster to be used to better understand these myopathies and suggest novel therapeutic strategies. Integrin-mediated adhesion is required to support muscle structure and f...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003406
更新日期:2013-03-01 00:00:00
abstract::Breast cancer is the most common cancer in women in developed countries, and the contribution of genetic susceptibility to breast cancer development has been well-recognized. However, a great proportion of these hereditary predisposing factors still remain unidentified. To examine the contribution of rare copy number ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002734
更新日期:2012-01-01 00:00:00
abstract::Populations in sub-Saharan Africa have historically been exposed to intense selection from chronic infection with falciparum malaria. Interestingly, populations with the highest malaria intensity can be identified by the increased occurrence of endemic Burkitt Lymphoma (eBL), a pediatric cancer that affects population...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008027
更新日期:2019-03-08 00:00:00
abstract::Complex trait genome-wide association studies (GWAS) provide an efficient strategy for evaluating large numbers of common variants in large numbers of individuals and for identifying trait-associated variants. Nevertheless, GWAS often leave much of the trait heritability unexplained. We hypothesized that some of this ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002198
更新日期:2011-07-01 00:00:00
abstract::During development of the cerebral cortex, neural stem cells (NSCs) divide symmetrically to proliferate and asymmetrically to generate neurons. Although faithful segregation of mitotic chromosomes is critical for NSC divisions, its fundamental mechanism remains unclear. A class of evolutionarily conserved protein comp...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004847
更新日期:2014-12-04 00:00:00
abstract::During neurogenesis, transcription factors combinatorially specify neuronal fates and then differentiate subtype identities by inducing subtype-specific gene expression profiles. But how is neuronal subtype identity maintained in mature neurons? Modeling this question in two Drosophila neuronal subtypes (Tv1 and Tv4),...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002501
更新日期:2012-01-01 00:00:00
abstract::Copy number expansions such as amplifications and duplications contribute to human phenotypic variation, promote molecular diversification during evolution, and drive the initiation and/or progression of various cancers. The mechanisms underlying these copy number changes are still incompletely understood, however. We...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003192
更新日期:2013-01-01 00:00:00