Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar.

Abstract:

BACKGROUND:Two thaumatin-like proteins (TLPs) were previously identified in phloem exudate of hybrid poplar (Populus trichocarpa x P. deltoides) using proteomics methods, and their sieve element localization confirmed by immunofluorescence. In the current study, we analyzed different tissues to further understand TLP expression and localization in poplar, and used immunogold labelling to determine intracellular localization. RESULTS:Immunofluorescence using a TLP antiserum confirmed the presence of TLP in punctate, organelle-like structures within sieve elements. On western blots, the antiserum labeled two constitutively expressed proteins with distinct expression patterns. Immunogold labelling suggested that TLPs are associated with starch granules and starch-containing plastids in sieve elements and phloem parenchyma cells. In addition, the antiserum recognized TLPs in the inner cell wall and sieve plate region of sieve elements. CONCLUSIONS:TLP localization in poplar cells and tissues is complex. TLP1 is expressed predominantly in tissues with a prominent vascular system such as midveins, petioles and stems, whereas the second TLP is primarily expressed in starch-storing plastids found in young leaves and the shoot apex.

journal_name

BMC Plant Biol

journal_title

BMC plant biology

authors

Dafoe NJ,Gowen BE,Constabel CP

doi

10.1186/1471-2229-10-191

subject

Has Abstract

pub_date

2010-08-26 00:00:00

pages

191

issn

1471-2229

pii

1471-2229-10-191

journal_volume

10

pub_type

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