Abstract:
BACKGROUND:In plants, eIF4E translation initiation factors and their eIFiso4E isoforms are essential susceptibility factors for many RNA viruses, including potyviruses. Mutations altering these factors are a major source of resistance to the viruses. The eIF4E allelic series is associated with specific resistance spectra in crops such as Capsicum annum. Genetic evidence shows that potyviruses have a specific requirement for a given 4E isoform that depends on the host plant. For example, Tobacco etch virus (TEV) uses eIF4E1 to infect Capsicum annuum but uses eIFiso4E to infect Arabidopsis thaliana. Here, we investigated how TEV exploits different translation initiation factor isoforms to infect these two plant species. RESULTS:A complementation system was set up in Arabidopsis to test the restoration of systemic infection by TEV. Using this system, Arabidopsis susceptibility to TEV was complemented with a susceptible pepper eIF4E1 allele but not with a resistant allele. Therefore, in Arabidopsis, TEV can use the pepper eIF4E1 instead of the endogenous eIFiso4E isoform so is able to switch between translation initiation factor 4E isoform to infect the same host. Moreover, we show that overexpressing the pepper eIF4E1 alleles is sufficient to make Arabidopsis susceptible to an otherwise incompatible TEV strain. Lastly, we show that the resistant eIF4E1 allele is similarly overcome by a resistance-breaking TEV strain as in pepper, confirming that this Arabidopsis TEV-susceptibility complementation system is allele-specific. CONCLUSION:We report here a complementation system in Arabidopsis that makes it possible to assess the role of pepper pvr2-eIF4E alleles in susceptibility to TEV. Heterologous complementation experiments showed that the idiosyncratic properties of the 4E and iso4E proteins create a major checkpoint for viral infection of different hosts. This system could be used to screen natural or induced eIF4E alleles to find and study alleles of interest for plant breeding.
journal_name
BMC Plant Bioljournal_title
BMC plant biologyauthors
Estevan J,Maréna A,Callot C,Lacombe S,Moretti A,Caranta C,Gallois JLdoi
10.1186/1471-2229-14-67subject
Has Abstractpub_date
2014-03-19 00:00:00pages
67issn
1471-2229pii
1471-2229-14-67journal_volume
14pub_type
杂志文章abstract:BACKGROUND:Herbivory imposes an important selective pressure on plants. In Arabidopsis thaliana leaf trichomes provide a key defense against insect herbivory; however, trichome production incurs a fitness cost in the absence of herbivory. Previous work on A. thaliana has shown an increase in trichome density in respons...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-14-119
更新日期:2014-05-05 00:00:00
abstract:BACKGROUND:Salt stress is one of the major abiotic stresses affecting plant growth and productivity. Vacuolar H+-pyrophosphatase (H+-PPase) genes play an important role in salt stress tolerance in multiple species. RESULTS:In this study, the promoter from the vacuolar H+-pyrophosphatase from Thellungiella halophila (T...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-90
更新日期:2010-05-18 00:00:00
abstract:BACKGROUND:Plant breeding has been proposed as one of the most effective and environmentally safe methods to control fungal infection and to reduce fumonisin accumulation. However, conventional breeding can be hampered by the complex genetic architecture of resistance to fumonisin accumulation and marker-assisted selec...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-1759-1
更新日期:2019-04-27 00:00:00
abstract:BACKGROUND:MicroRNAs play important roles in the adaptive responses of plants to nutrient deficiencies. Most research, however, has focused on nitrogen (N), phosphorus (P), sulfur (S), copper (Cu) and iron (Fe) deficiencies, limited data are available on the differential expression of miRNAs and their target genes in r...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-015-0642-y
更新日期:2015-11-04 00:00:00
abstract:BACKGROUND:Rice is highly sensitive to temperature fluctuations. Recently, the frequent occurrence of high temperature stress has heavily influenced rice production. Proper heading date in specific environmental conditions could ensure high grain yield. Rice heading greatly depends on the accurate measurement of enviro...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-018-1330-5
更新日期:2018-06-07 00:00:00
abstract:BACKGROUND:Plant defensins are small (45-54 amino acids), basic, cysteine-rich proteins that have a major role in innate immunity in plants. Many defensins are potent antifungal molecules and are being evaluated for their potential to create crop plants with sustainable disease resistance. Defensins are produced as pre...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-14-41
更新日期:2014-02-05 00:00:00
abstract:BACKGROUND:An interesting seed protein family with a role in preventing insect herbivory is the multi-gene, APA family encoding the alpha-amylase inhibitor, phytohemagglutinin and arcelin proteins of common bean (Phaseolus vulgaris). Variability for this gene family exists and has been exploited to breed for insect res...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-79
更新日期:2010-04-29 00:00:00
abstract:BACKGROUND:Strawberry (Fragaria × ananassa Duch.) is an important fruit crop worldwide. It was particularly sensitive to drought stress because of their fibrous and shallow root systems. Mutant rty of Arabidopsis thaliana ROOTY (RTY) results in increased endogenous auxin levels, more roots, and shoot growth. It is stil...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-021-02839-4
更新日期:2021-01-21 00:00:00
abstract:BACKGROUND:Cytokinin Response Factors (CRFs) are a small subset of AP2/ERF transcription factor genes shown in Arabidopsis to regulate leaf development as part of the cytokinin signal transduction pathway. This study examines the phylogenetic distribution of CRF genes in other plant species, and attempts to identify th...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-74
更新日期:2010-04-26 00:00:00
abstract:BACKGROUND:Alternate bearing is a widespread phenomenon among crop plants, defined as the tendency of certain fruit trees to produce a high-yield crop one year ("on-year"), followed by a low-yield or even no crop the following year ("off-year"). Several factors may affect the balance between such developmental phase-tr...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-13-10
更新日期:2013-01-15 00:00:00
abstract:BACKGROUND:Long noncoding RNAs (lncRNAs) have several known functions involving various biological regulatory processes in plant. However, the possible roles of lncRNAs during peanut seed development have not been fully explored. RESULTS:In this study, two peanut recombinant inbred lines (RIL8) that differ in seed siz...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-020-02405-4
更新日期:2020-05-06 00:00:00
abstract:BACKGROUND:Chickpea (Cicer arietinum L.) is the second most widely grown pulse and drought (limiting water) is one of the major constraints leading to about 40-50% yield losses annually. Dehydration responsive element binding proteins (DREBs) are important plant transcription factors that regulate the expression of man...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-020-02815-4
更新日期:2021-01-11 00:00:00
abstract:BACKGROUND:Multiple hybridization events gave rise to pentaploid dogroses which can reproduce sexually despite their uneven ploidy level by the unique canina meiosis. Two homologous chromosome sets are involved in bivalent formation and are transmitted by the haploid pollen grains and the tetraploid egg cells. In addit...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-11-37
更新日期:2011-02-23 00:00:00
abstract:BACKGROUND:Currently, Tectona grandis is one of the most valuable trees in the world and no transcript dataset related to secondary xylem is available. Considering how important the secondary xylem and sapwood transition from young to mature trees is, little is known about the expression differences between those succe...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-015-0599-x
更新日期:2015-09-15 00:00:00
abstract:BACKGROUND:Sugar beet is a highly salt-tolerant crop. However, its ability to withstand high salinity is reduced compared to sea beet, a wild ancestor of all beet crops. The aim of this study was to investigate transcriptional patterns associated with physiological, cytological and biochemical mechanisms involved in sa...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-1661-x
更新日期:2019-02-06 00:00:00
abstract:BACKGROUND:Sucrose synthase (SUS) is widely considered a key enzyme participating in sucrose metabolism in higher plants and regarded as a biochemical marker for sink strength in crops. However, despite significant progress in characterizing the physiological functions of the SUS gene family, knowledge of the trajector...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-2181-4
更新日期:2019-12-18 00:00:00
abstract:BACKGROUND:Maize (Zea mays) is an important model crop for transgenic studies. However, genetic transformation of maize requires embryonic calli derived from immature embryo, and the impact of utilizing tissue culture methods on the maize epigenome is poorly understood. Here, we generated whole-genome MeDIP-seq data ex...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-017-1055-x
更新日期:2017-06-15 00:00:00
abstract:BACKGROUND:From field harvest to the consumer's table, fresh citrus fruit spends a considerable amount of time in shipment and storage. During these processes, physiological disorders and pathological diseases are the main causes of fruit loss. Heat treatment (HT) has been widely used to maintain fruit quality during p...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-13-44
更新日期:2013-03-16 00:00:00
abstract:BACKGROUND:Extremely high temperatures are becoming an increasingly severe threat to crop yields. It is well documented that salicylic acid (SA) can enhance the stress tolerance of plants; however, its effect on the reproductive organs of rice plants has not been described before. To investigate the mechanism underlyin...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-018-1472-5
更新日期:2018-10-19 00:00:00
abstract:BACKGROUND:Fruit coloration of red-skinned grapevines is mainly due to anthocyanin pigments. We analysed a panel of nine cultivars that included extreme phenotypes for berry colour, ranging from green (absence of anthocyanins) to red, purple, violet and blue. Expression of six genes of the anthocyanin pathway coding fo...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-7-46
更新日期:2007-08-30 00:00:00
abstract:BACKGROUND:Protein synthesis is a highly energy demanding process and is regulated according to cellular energy levels. Light and sugar availability affect mRNA translation in plant cells but the specific roles of these factors remain unclear. In this study, sucrose was applied to Arabidopsis seedlings kept in the ligh...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-014-0306-3
更新日期:2014-11-18 00:00:00
abstract:BACKGROUND:DNA ligase enzymes catalyse the joining of adjacent polynucleotides and as such play important roles in DNA replication and repair pathways. Eukaryotes possess multiple DNA ligases with distinct roles in DNA metabolism, with clear differences in the functions of DNA ligase orthologues between animals, yeast ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-9-79
更新日期:2009-06-26 00:00:00
abstract:BACKGROUND:The sweet, floral flavor typical of Muscat varieties (Muscats), due to high levels of monoterpenoids (geraniol, linalool and nerol), is highly distinct and has been greatly appreciated both in table grapes and in wine since ancient times. Muscat flavor determination in grape (Vitis vinifera L.) has up to now...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-241
更新日期:2010-11-09 00:00:00
abstract:BACKGROUND:Photosynthesis plays a vital role as an energy source for plant metabolism, and its efficiency may be drastically reduced owing to abiotic stresses. Moso bamboo (Phyllostachys edulis), is a renewable and versatile resource with significant ecological and economic value, which encounters high light stress wit...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-016-0720-9
更新日期:2016-01-28 00:00:00
abstract:BACKGROUND:Genome-wide association studies (GWAS) based on linkage disequilibrium (LD) provide a promising tool for the detection and fine mapping of quantitative trait loci (QTL) underlying complex agronomic traits. In this study we explored the genetic basis of variation for the traits heading date, plant height, tho...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-12-16
更新日期:2012-01-27 00:00:00
abstract:BACKGROUND:Oil palm is the world's most productive oil-food crop despite yielding well below its theoretical maximum. This maximum could be approached with the introduction of elite F1 varieties. The development of such elite lines has thus far been prevented by difficulties in generating homozygous parental types for ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-218
更新日期:2010-10-07 00:00:00
abstract:BACKGROUND:Pongamia (Millettia pinnata syn. Pongamia pinnata), an oilseed legume species, is emerging as potential feedstock for sustainable biodiesel production. Breeding Pongamia for favorable traits in commercial application will rely on a comprehensive understanding of molecular mechanism regulating oil accumulatio...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-018-1356-8
更新日期:2018-07-09 00:00:00
abstract:BACKGROUND:Angiosperm sex chromosomes, where present, are generally recently evolved. The key step in initiating the development of sex chromosomes from autosomes is the establishment of a sex-determining locus within a region of non-recombination. To better understand early sex chromosome evolution, it is important to...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-1766-2
更新日期:2019-04-30 00:00:00
abstract:BACKGROUND:Although a number of leucine-rich repeat receptor-like kinase-encoding genes (LRR-RLKs) have been identified in plants, a functional role has been determined for only a few. Recent studies have demonstrated that an LRR-RLK, PXY/TDR, is important for the process of secondary vascular development. Other studie...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-13-94
更新日期:2013-07-01 00:00:00
abstract:BACKGROUND:Flax (Linum usitatissimum L.) is an economically important fiber and oil crop that has been grown for thousands of years. The genome has been recently sequenced and transcriptomics are providing information on candidate genes potentially related to agronomically-important traits. In order to accelerate funct...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-13-159
更新日期:2013-10-15 00:00:00