Abstract:
BACKGROUND:Pongamia (Millettia pinnata syn. Pongamia pinnata), an oilseed legume species, is emerging as potential feedstock for sustainable biodiesel production. Breeding Pongamia for favorable traits in commercial application will rely on a comprehensive understanding of molecular mechanism regulating oil accumulation during its seed development. To date, only limited genomic or transcript sequences are available for Pongamia, while a temporal transcriptome profiling of developing seeds is still lacking in this species. RESULTS:In this work, we conducted a time-series analysis of morphological and physiological characters, oil contents and compositions, as well as global gene expression profiles in developing Pongamia seeds. Firstly, three major developmental phases were characterized based on the combined evidences from embryonic shape, seed weight, seed moisture content, and seed color. Then, the gene expression levels at these three phases were quantified by RNA-Seq analyses with three biological replicates from each phase. Nearly 94% of unigenes were expressed at all three phases, whereas only less than 2% of unigenes were exclusively expressed at one of these phases. A total of 8881 differentially expressed genes (DEGs) were identified between phases. Furthermore, the qRT-PCR analyses for 10 DEGs involved in lipid metabolism demonstrated a good reliability of our RNA-Seq data in temporal gene expression profiling. We observed a dramatic increase in seed oil content from the embryogenesis phase to the early seed-filling phase, followed by a steady and moderate increase towards the maximum at the desiccation phase. We proposed that a highly active expression of most genes related to fatty acid (FA) and triacylglycerol (TAG) biosynthesis at the embryogenesis phase might trigger both the substantial oil accumulation and the membrane lipid synthesis for rapid cell proliferation at this phase, while a concerted reactivation of TAG synthesis-related genes at the desiccation phase might further promote storage lipid synthesis to achieve the maximum content of seed oils. CONCLUSIONS:This study not only built a bridge between gene expression profiles and oil accumulation in developing seeds, but also laid a foundation for future attempts on genetic engineering of Pongamia varieties to acquire higher oil yield or improved oil properties for biofuel applications.
journal_name
BMC Plant Bioljournal_title
BMC plant biologyauthors
Huang J,Hao X,Jin Y,Guo X,Shao Q,Kumar KS,Ahlawat YK,Harry DE,Joshi CP,Zheng Ydoi
10.1186/s12870-018-1356-8subject
Has Abstractpub_date
2018-07-09 00:00:00pages
140issue
1issn
1471-2229pii
10.1186/s12870-018-1356-8journal_volume
18pub_type
杂志文章abstract:BACKGROUND:Cattail (Typha domingensis Pers.) is a perennial emergent plant which is used in Green Floating Filters (GFFs), one of the most innovative systems of wastewater treatment to bioremediate eutrophic waters and produce biomass as biofuel feedstocks. The establishment of cattails in GFFs depends on the seed germ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-020-02573-3
更新日期:2020-08-17 00:00:00
abstract:BACKGROUND:Characterization of the expression and function of genes regulating embryo development in conifers is interesting from an evolutionary point of view. However, our knowledge about the regulation of embryo development in conifers is limited. During early embryo development in Pinus species the proembyo goes th...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-016-0939-5
更新日期:2016-11-18 00:00:00
abstract:BACKGROUND:The translocator protein 18 kDa (TSPO), previously known as the peripheral-type benzodiazepine receptor (PBR), is important for many cellular functions in mammals and bacteria, such as steroid biosynthesis, cellular respiration, cell proliferation, apoptosis, immunomodulation, transport of porphyrins and ani...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-11-108
更新日期:2011-06-20 00:00:00
abstract:BACKGROUND:An interesting seed protein family with a role in preventing insect herbivory is the multi-gene, APA family encoding the alpha-amylase inhibitor, phytohemagglutinin and arcelin proteins of common bean (Phaseolus vulgaris). Variability for this gene family exists and has been exploited to breed for insect res...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-79
更新日期:2010-04-29 00:00:00
abstract:BACKGROUND:CRISPR/Cas9 gene editing has become a revolutionary technique for crop improvement as it can facilitate fast and efficient genetic changes without the retention of transgene components in the final plant line. Lack of robust bioinformatics tools to facilitate the design of highly specific functional guide RN...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-2097-z
更新日期:2019-11-06 00:00:00
abstract:BACKGROUND:Among the various biochemical markers, fatty acids or lipid profiles represent a chemically relatively inert class of compounds that is easy to isolate from biological material. Fatty acid (FA) profiles are considered as chemotaxonomic markers to define groups of various taxonomic ranks in flowering plants, ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-11-124
更新日期:2011-09-06 00:00:00
abstract::Classical plant breeding was extremely successful in generating high yielding crop varieties. Yet, in modern crops, the long domestication process has impoverished the genetic diversity available for breeding. This is limiting further improvements of elite germplasm by classical approaches. The CRISPR/Cas system now e...
journal_title:BMC plant biology
pub_type: 杂志文章,评审
doi:10.1186/s12870-019-1775-1
更新日期:2019-05-02 00:00:00
abstract:BACKGROUND:Haematococcus lacustris is an ideal source of astaxanthin (AST), which is stored in oil bodies containing esterified AST (EAST) and triacylglycerol (TAG). Diacylglycerol acyltransferases (DGATs) catalyze the last step of acyl-CoA-dependent TAG biosynthesis and are also considered as crucial enzymes involved ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-020-02794-6
更新日期:2021-01-06 00:00:00
abstract:BACKGROUND:Vacuolar invertases (VINs) have been reported to regulate plant growth and development and respond to abiotic stresses such as drought and cold. With our best knowledge, the functions of VIN genes little have been reported in tea plant (Camellia sinensis L.). Therefore, it is necessary to develop research in...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-018-1456-5
更新日期:2018-10-11 00:00:00
abstract:BACKGROUND:Jasmonates play an important role in plant stress and defence responses and are also involved in the regulation of anthocyanin synthesis in response to sucrose availability. Here we explore the signalling interactions between sucrose and jasmonates in response to cold stress in Arabidopsis. RESULTS:Sucrose ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-020-02376-6
更新日期:2020-04-22 00:00:00
abstract:BACKGROUND:Chromosome pairing, recombination and DNA repair are essential processes during meiosis in sexually reproducing organisms. Investigating the bread wheat (Triticum aestivum L.) Ph2 (Pairing homoeologous) locus has identified numerous candidate genes that may have a role in controlling such processes, includin...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-7-67
更新日期:2007-12-20 00:00:00
abstract:BACKGROUND:Leaves of Poaceae have a unique morphological feature: they consist of a proximal sheath and a distal blade separated by a ligular region. The sheath provides structural support and protects young developing leaves, whereas the main function of the blade is photosynthesis. The auricles allow the blade to til...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-1635-z
更新日期:2019-02-15 00:00:00
abstract:BACKGROUND:Gynodioecious species exist in two sexes - male-sterile females and hermaphrodites. Male sterility in higher plants often results from mitonuclear interaction between the CMS (cytoplasmic male sterility) gene(s) encoded by mitochondrial genome and by nuclear-encoded restorer genes. Mitochondrial and nuclear-...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-2193-0
更新日期:2019-12-19 00:00:00
abstract:BACKGROUND:Cytokinin Response Factors (CRFs) are a small subset of AP2/ERF transcription factor genes shown in Arabidopsis to regulate leaf development as part of the cytokinin signal transduction pathway. This study examines the phylogenetic distribution of CRF genes in other plant species, and attempts to identify th...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-74
更新日期:2010-04-26 00:00:00
abstract:BACKGROUND:Glutamine synthetase (GS) acts as a key enzyme in plant nitrogen (N) metabolism. It is important to understand the regulation of GS expression in plant. Promoters can initiate the transcription of its downstream gene. Eichhornia crassipes is a most prominent aquatic invasive plant, which has negative effects...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-021-02832-x
更新日期:2021-01-29 00:00:00
abstract:BACKGROUND:Manganese (Mn), an essential element for plants, can be toxic when present in excess. Stylo (Stylosanthes) is a pioneer tropical legume with great potential for Mn tolerance, but its Mn tolerance mechanisms remain poorly understood. RESULTS:In this study, variations in Mn tolerance were observed among nine ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-1822-y
更新日期:2019-05-22 00:00:00
abstract:BACKGROUND:Sweet potato (Ipomoea batatas L.) is the sixth most important food crop in the world. The formation and development of storage roots in sweet potato is a highly complicated and genetically programmed process. However, the underlying mechanisms of storage root development have not yet been elucidated. RESULT...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-1731-0
更新日期:2019-04-11 00:00:00
abstract:BACKGROUND:NADP-malic enzyme (NAPD-ME), and pyruvate orthophosphate dikinase (PPDK) are important enzymes that participate in C4 photosynthesis. However, the evolutionary history and forces driving evolution of these genes in C4 plants are not completely understood. RESULTS:We identified 162 NADP-ME and 35 PPDK genes ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-020-02339-x
更新日期:2020-03-30 00:00:00
abstract:BACKGROUND:Plant microRNAs (miRNAs) are a class of small, non-coding RNAs that play an important role in development and environmental responses. Hundreds of plant miRNAs have been identified to date, mainly from the model species for which there are available genome sequences. The current challenge is to characterise ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-159
更新日期:2010-08-04 00:00:00
abstract:BACKGROUND:miR399 and miR827 are both involved in conserved phosphorus (P) deficiency signalling pathways. miR399 targets the PHO2 gene encoding E2 enzyme that negatively regulates phosphate uptake and root-to-shoot allocation, while miR827 targets SPX-domain-containing genes that negatively regulate other P-responsive...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-13-214
更新日期:2013-12-13 00:00:00
abstract:BACKGROUND:Epigenetic factors such as DNA methylation and histone modifications regulate a wide range of processes in plant development. Cytosine methylation and demethylation exist in a dynamic balance and have been associated with gene silencing or activation, respectively. In Arabidopsis, cytosine demethylation is a...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-13-172
更新日期:2013-10-31 00:00:00
abstract:BACKGROUND:Assembly of stable light-harvesting complexes (LHCs) in the chloroplast of green algae and plants requires synthesis of chlorophyll (Chl) b, a reaction that involves oxygenation of the 7-methyl group of Chl a to a formyl group. This reaction uses molecular oxygen and is catalyzed by chlorophyllide a oxygenas...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-4-5
更新日期:2004-04-15 00:00:00
abstract:BACKGROUND:Wheat grain avenin-like proteins (ALPs) belong to a recently discovered class of wheat grain storage protein. ALPs in wheat grains not only have beneficial effects on dough quality but also display antifungal activities, which is a novel observation for wheat storage proteins. Previous studies have shown tha...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-020-2259-z
更新日期:2020-01-29 00:00:00
abstract:BACKGROUND:Paphiopedilum is a horticulturally and ecologically important genus of ca. 80 species of lady's slipper orchids native to Southeast Asia. These plants have long been of interest regarding their chromosomal evolution, which involves a progressive aneuploid series based on either fission or fusion of centromer...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-11-126
更新日期:2011-09-12 00:00:00
abstract:BACKGROUND:The phloem of dicotyledonous plants contains specialized P-proteins (phloem proteins) that accumulate during sieve element differentiation and remain parietally associated with the cisternae of the endoplasmic reticulum in mature sieve elements. Wounding causes P-protein filaments to accumulate at the sieve ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-219
更新日期:2010-10-08 00:00:00
abstract:BACKGROUND:Rhododendron molle (Ericaceae) is a traditional Chinese medicinal plant, its flower and root have been widely used to treat rheumatism and relieve pain for thousands of years in China. Chemical studies have revealed that R. molle contains abundant secondary metabolites such as terpenoinds, flavonoids and lig...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-020-02586-y
更新日期:2020-09-04 00:00:00
abstract:BACKGROUND:Hordeum chilense, a native South American diploid wild barley, is one of the species of the genus Hordeum with a high potential for cereal breeding purposes, given its high crossability with other members of the Triticeae tribe. Hexaploid tritordeum (×Tritordeum Ascherson et Graebner, 2n=6×=42, AABBH(ch)H(ch...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-13-87
更新日期:2013-06-03 00:00:00
abstract:BACKGROUND:Flavonoids have essential roles in flower pigmentation, fibre development and disease resistance in cotton. Previous studies show that accumulation of naringenin in developing cotton fibres significantly affects fibre growth. This study focused on determining the effects of the flavonoids naringenin, dihydro...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-2054-x
更新日期:2019-10-24 00:00:00
abstract:BACKGROUND:The hypersensitive necrosis response (HR) of resistant plants to avirulent pathogens is a form of programmed cell death in which the plant sacrifices a few cells under attack, restricting pathogen growth into adjacent healthy tissues. In spite of the importance of this defense response, relatively little is ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-7-35
更新日期:2007-07-06 00:00:00
abstract:BACKGROUND:Cultivated peanut (Arachis hypogaea. L) is one of the most important oil crops in the world. After flowering, the peanut plant forms aboveground pegs (gynophores) that penetrate the soil, giving rise to underground pods. This means of reproduction, referred to as geocarpy, distinguishes peanuts from most oth...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-015-0582-6
更新日期:2015-08-04 00:00:00