Higher expression of the heterogeneous nuclear ribonucleoprotein k in melanoma.

Abstract:

BACKGROUND:The heterogeneous nuclear ribonucleoprotein (hnRNP) K is an essential RNA and DNA binding protein involved in gene expression and signal transduction. The role of hnRNP K in cancer is relatively understudied. However, several cellular functions strongly indicate that hnRNP K is involved in tumorigenesis. Oncogenes c-Src, c-myc, and eIF4E are regulated by hnRNP K. We have shown an increased cytoplasmic hnRNP K in pancreatic cancer. In the present study, we investigated the altered expression of hnRNP K protein and its correlation with p-ERK in melanoma using human melanoma cell lines and tissue microarray. MATERIALS AND METHODS:The protein levels of hnRNP K and p-ERK in 8 human melanoma cell lines and a melanoma progression tissue microarray containing 80 melanoma, 23 dysplastic nevi, and 14 benign nevi specimens were analyzed using Western blot and immunohistochemistry analysis. hnRNP K was knocked down by siRNA, and its effect on melanoma cells was assessed. RESULTS:We showed a higher hnRNP K protein level in both melanoma cell lines and melanoma tissue specimens, which correlated with a higher c-myc expression. An increase in the cytoplasmic hnRNP K and eIF4E protein levels in melanoma cells is also seen. p-ERK level was also higher in dysplastic nevi and melanoma tissues, but did not correlate with hnRNP K protein level. We then demonstrated that knocking down of hnRNP K by siRNA inhibited melanoma cell growth and colony formation, as well as c-myc expression. CONCLUSIONS:hnRNP K expression correlated with melanoma and may play a role in melanoma tumorigenesis.

journal_name

Ann Surg Oncol

authors

Wen F,Shen A,Shanas R,Bhattacharyya A,Lian F,Hostetter G,Shi J

doi

10.1245/s10434-010-1121-1

subject

Has Abstract

pub_date

2010-10-01 00:00:00

pages

2619-27

issue

10

eissn

1068-9265

issn

1534-4681

journal_volume

17

pub_type

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