Developmental regulation of beta-thymosins in the rat central nervous system.

abstract：:Procedures are described for the solubilization of adenosine uptake sites in guinea pig and rat brain tissue. Using [3H]nitrobenzylthioinosine [( 3H]NBI) the solubilized site is characterized both kinetically and pharmacologically. The binding is dependent on protein concentration and is saturable, reversible, specifi...

journal_title：Journal of neurochemistry

authors： Verma A,Houston M,Marangos PJ

更新日期：1985-08-01 00:00:00

abstract：:A rapid and simple procedure is presented to obtain nearly pure populations of human neuron-like cells from the SH-SY5Y neuroblastoma cell line. Sequential exposure of SH-SY5Y cells to retinoic acid and brain-derived neurotrophic factor in serum-free medium yields homogeneous populations of cells with neuronal morphol...

journal_title：Journal of neurochemistry

authors： Encinas M,Iglesias M,Liu Y,Wang H,Muhaisen A,Ceña V,Gallego C,Comella JX

更新日期：2000-09-01 00:00:00

abstract：:A mass fragmentographic method for the determination of trace amounts of amino acid neurotransmitter candidates from brain perfusates is described. The analytical procedure includes the measurements of glycine, beta-alanine, gamma-aminobutyric acid, proline, aspartic acid, and glutamic acid; alpha-alanine, leucine, an...

journal_title：Journal of neurochemistry

authors： Wolfensberger M,Amsler U

更新日期：1982-02-01 00:00:00

abstract：:Previous work from our laboratory points to plasma free tryptophan being a useful predictor of brain tryptophan concentration in many circumstances. Other work, in particular various studies on the acute effects of food intake, has emphasized the roles of plasma total tryptophan and of plasma large neutral amino acids...

journal_title：Journal of neurochemistry

authors： Sarna GS,Kantamaneni BD,Curzon G

更新日期：1985-05-01 00:00:00

abstract：:Type 1 cannabinoid receptor (CB1) is expressed in different neuronal populations in the mammalian brain. In particular, CB1 on GABAergic or glutamatergic neurons exerts different functions and display different pharmacological properties in vivo. This suggests the existence of neuron-type specific signalling pathways ...

journal_title：Journal of neurochemistry

authors： Steindel F,Lerner R,Häring M,Ruehle S,Marsicano G,Lutz B,Monory K

更新日期：2013-03-01 00:00:00

abstract：:Cavernous angiomas are vascular malformations that cause neurodegeneration and symptoms including epileptiform seizures, headache, and motor deficits. Following neurosurgical removal of the angiomas, patients mostly recover well and become seizure-free. This study reports on the levels of certain amino acids in angiom...

journal_title：Journal of neurochemistry

authors： von Essen C,Rydenhag B,Nyström B,Mozzi R,van Gelder N,Hamberger A

更新日期：1996-07-01 00:00:00

abstract：:GTP binding regulatory protein (G protein)-coupled receptors can activate MAPK pathways via G protein-dependent and -independent mechanisms. However, the physiological outcomes correlated with the cellular signaling events are not as well characterized. In this study, we examine the involvement of G protein and beta-a...

journal_title：Journal of neurochemistry

authors： McLennan GP,Kiss A,Miyatake M,Belcheva MM,Chambers KT,Pozek JJ,Mohabbat Y,Moyer RA,Bohn LM,Coscia CJ

更新日期：2008-12-01 00:00:00

abstract：:Agmatine (decarboxylated arginine) was originally identified in the CNS as an imidazoline receptor ligand. Further studies demonstrated that agmatine antagonizes NMDA receptors and inhibits nitric oxide synthase. Intrathecally administered agmatine inhibits opioid tolerance and hyperalgesia evoked by inflammation, ner...

journal_title：Journal of neurochemistry

authors： Goracke-Postle CJ,Overland AC,Riedl MS,Stone LS,Fairbanks CA

更新日期：2007-09-01 00:00:00

abstract：:The extracellular concentrations of amino acids in the hippocampal CA1 field and striatum of conscious freely moving rats were monitored simultaneously by in vivo brain microdialysis using HPLC with electrochemical detection. Under basal conditions, aspartate, glutamate, glutamine, glycine, taurine, and alanine were d...

journal_title：Journal of neurochemistry

authors： Shibanoki S,Kogure M,Sugahara M,Ishikawa K

更新日期：1993-11-01 00:00:00

abstract：:Mitochondrial dysfunction represents a critical event during the pathogenesis of Parkinson's disease (PD) and expanding evidences demonstrate that an altered balance in mitochondrial fission/fusion is likely an important mechanism leading to mitochondrial and neuronal dysfunction/degeneration. In this study, we invest...

journal_title：Journal of neurochemistry

authors： Wang X,Petrie TG,Liu Y,Liu J,Fujioka H,Zhu X

更新日期：2012-06-01 00:00:00

abstract：:The deficits in Alzheimer disease (AD) stem at least partly from neurotoxic beta-amyloid peptides generated from the amyloid precursor protein (APP). APP may also be cleaved intracellularly at Asp664 to yield a second neurotoxic peptide, C31. Previously, we showed that cleavage of APP at the C-terminus is required for...

journal_title：Journal of neurochemistry

authors： Nguyen TV,Galvan V,Huang W,Banwait S,Tang H,Zhang J,Bredesen DE

更新日期：2008-02-01 00:00:00

abstract：:Diadenosine tetraphosphate (Ap4A) and diadenosine pentaphosphate (Ap5A) have been identified in bovine adrenal medullary tissue using an HPLC method. The values obtained were 0.1 +/- 0.05 mumol/g of tissue for both compounds. The subcellular fraction where Ap4A and Ap5A were present in the highest concentration was ch...

journal_title：Journal of neurochemistry

authors： Rodriguez del Castillo A,Torres M,Delicado EG,Miras-Portugal MT

更新日期：1988-12-01 00:00:00

abstract：:Purkinje cell degeneration (pcd) was first identified in a spontaneous mouse mutant showing cerebellar ataxia. In addition to cerebellar Purkinje cells (PCs), retinal photoreceptors, mitral cells in the olfactory bulb, and a discrete subpopulation of thalamic neurons also degenerate in the mutant brains. The gene resp...

journal_title：Journal of neurochemistry

authors： Zhou L,Hossain MI,Yamazaki M,Abe M,Natsume R,Konno K,Kageyama S,Komatsu M,Watanabe M,Sakimura K,Takebayashi H

更新日期：2018-11-01 00:00:00

abstract：:The biosynthesis of angiotensin II-like peptide was measured in primary cultured brain cells from the fetal rat. Cells from the whole brains of 20-day gestational age Sprague-Dawley rats were dissociated by mild trypsinization and grown for 5 days in supplemented (serum-free) medium prior to experimental analysis. The...

journal_title：Journal of neurochemistry

authors： Weyhenmeyer JA,Meyer JM,Watkins JE

更新日期：1984-09-01 00:00:00

abstract：:Spinocerebellar ataxia type 3 (SCA3) is one of at least nine inherited neurodegenerative diseases caused by an expansion of a polyglutamine tract within corresponding disease-specific proteins. In case of SCA3, mutation of Ataxin-3 results in aggregation of misfolded protein, formation of intranuclear as well as cytos...

journal_title：Journal of neurochemistry

authors： Liman J,Deeg S,Voigt A,Voßfeldt H,Dohm CP,Karch A,Weishaupt J,Schulz JB,Bähr M,Kermer P

更新日期：2014-06-01 00:00:00

abstract：:Recent neurochemical studies of the properties of 5-hydroxytryptamine (5-HT) pathways arising from the dorsal raphe nucleus (DRN) and median raphe nucleus (MRN) have measured extracellular 5-HT in brain regions with reported preferential DRN or MRN 5-HT inputs. Here, we have tested whether electrical stimulation of th...

journal_title：Journal of neurochemistry

authors： McQuade R,Sharp T

更新日期：1997-08-01 00:00:00

abstract：:Neurodegenerative diseases, such as Parkinson's disease, Alzheimer's disease, and Huntington's disease, are considered incurable and significantly reduce the quality of life of the patients. A variety of drugs that modulate neurotransmitter levels have been used for the treatment of the neurodegenerative diseases but ...

journal_title：Journal of neurochemistry

authors： Huang M,Rathore SS,Lindau M

更新日期：2019-10-01 00:00:00

abstract：:Limited information is available on the identity of antigens targeted by antibodies present in cerebrospinal fluid (CSF) of patients with clinically isolated syndrome (CIS). The aim of this study was to identify novel antigens for CIS and investigate their prognostic potential to predict conversion to multiple scleros...

journal_title：Journal of neurochemistry

authors： Rouwette M,Somers K,Govarts C,De Deyn PP,Hupperts R,Van Wijmeersch B,De Jong BA,Verbeek MM,Van Pesch V,Sindic C,Villar LM,Álvarez-Cermeño JC,Stinissen P,Somers V

更新日期：2012-11-01 00:00:00

abstract：:The NMDA receptor exhibits increased sensitivity to stimulation during early development compared with the adult. In this study, we examined modulation of the NMDA receptor by polyamines during development to see if it correlates with differences in the functional responsiveness of the NMDA receptor. [3H]MK-801 bindin...

journal_title：Journal of neurochemistry

authors： Subramaniam S,McGonigle P

更新日期：1994-04-01 00:00:00

abstract：:The beta-amyloid peptide (Abeta) is considered responsible for the pathogenesis of Alzheimer's disease. Despite the magnitude of reports describing a neurotoxic role of extracellular Abeta, the role for intracellular Abeta (iAbeta) has not been elucidated. We previously demonstrated that in rat pheochromocytoma cells ...

journal_title：Journal of neurochemistry

authors： Arvanitis DN,Ducatenzeiler A,Ou JN,Grodstein E,Andrews SD,Tendulkar SR,Ribeiro-da-Silva A,Szyf M,Cuello AC

更新日期：2007-10-01 00:00:00

abstract：:Phospholipids are asymmetrically distributed across the mammalian plasma membrane, with phosphatidylserine (PS) and phosphatidylethanolamine concentrated in the cytoplasmic leaflet of the membrane bilayer and phosphatidylcholine in the exoplasmic leaflet. This asymmetric distribution is dependent on a group of P4 ATPa...

journal_title：Journal of neurochemistry

authors： Yang Y,Liu W,Sun K,Jiang L,Zhu X

更新日期：2019-02-01 00:00:00

abstract：:Variations occurring in cortical nitric oxide (NO) release were analysed with a voltametric method in rats (i) placed in control conditions, (ii) while being paradoxical sleep deprived (PSD), or (iii) recovering from a PSD. Activities of neuronal (nNOS) and inducible (iNOS) NO-synthases as well as nNOS expression were...

journal_title：Journal of neurochemistry

authors： Clément P,Sarda N,Cespuglio R,Gharib A

更新日期：2004-08-01 00:00:00

abstract：:Parkinson's disease is a neurodegenerative movement disorder. The histopathology of Parkinson's disease comprises proteinaceous inclusions known as Lewy bodies, which contains aggregated α-synuclein. Cathepsin D (CD) is a lysosomal protease previously demonstrated to cleave α-synuclein and decrease its toxicity in bot...

journal_title：Journal of neurochemistry

authors： Crabtree D,Dodson M,Ouyang X,Boyer-Guittaut M,Liang Q,Ballestas ME,Fineberg N,Zhang J

更新日期：2014-03-01 00:00:00

abstract：:We examined changes in biosynthetic capacity of sciatic nerve during the early stages of Wallerian degeneration, utilizing a model that permits exclusion of nonresident cells from degenerating nerve. Sciatic nerve segments were placed in either 5-microns pore (allowing infiltration of nonresident cells) or 0.22-micron...

journal_title：Journal of neurochemistry

authors： White FV,Toews AD,Goodrum JF,Novicki DL,Bouldin TW,Morell P

更新日期：1989-04-01 00:00:00

abstract：:GD3 synthase (Sial-T2) is a key enzyme of ganglioside synthesis that, in concert with GM2 synthase (GalNAc-T), regulates the ratio of a- and b-pathway gangliosides. In this work, we study the sub-Golgi location of an epitope-tagged version of chicken Sial-T2 transfected to CHO-K1 cells. The expressed protein was enzym...

journal_title：Journal of neurochemistry

authors： Daniotti JL,Martina JA,Giraudo CG,Zurita AR,Maccioni HJ

更新日期：2000-04-01 00:00:00

abstract：:Microtubule-associated protein (MAP) 1B is a high-molecular-weight cytoskeletal protein that is abundant in developing neuronal processes and appears to be necessary for axonal growth. Various biochemical and immunocytochemical results are reported, indicating that a significant fraction of MAP1B is expressed as an in...

journal_title：Journal of neurochemistry

authors： Tanner SL,Franzen R,Jaffe H,Quarles RH

更新日期：2000-08-01 00:00:00

abstract：:Nitric oxide (.NO) synthase (NOS) was induced in cultured rat astrocytes by incubation with lipopolysaccharide (LPS) for 18 h and gap junction permeability was assessed by the scrape-loading/Lucifer yellow transfer technique. Induction of NOS was confirmed by determining either the NG-methyl-L-arginine (NMMA)-inhibita...

journal_title：Journal of neurochemistry

authors： Bolaños JP,Medina JM

更新日期：1996-05-01 00:00:00

abstract：:The fate of gonadotropin-releasing hormone (GnRH) was examined by a GnRH radioimmunoassay during in vitro incubations of the rat medial basal hypothalamus (MBH). There was a progressive disappearance of exogenous GnRH during MBH incubations. The GnRH degradation could be explained by the release of peptidases from the...

journal_title：Journal of neurochemistry

authors： Powers CA,Johnson DC

更新日期：1981-02-01 00:00:00

abstract：:The present study demonstrates a conditional, agonist-dependent phosphorylation of the mu-opioid receptor (MOR-1) by cyclic AMP-dependent protein kinase (PKA) in membrane preparations of MOR-1-transfected neuroblastoma Neuro2A cells. Opioid agonist-dependent phosphorylation occurs in a time- and concentration-dependen...

journal_title：Journal of neurochemistry

authors： Chakrabarti S,Law PY,Loh HH

更新日期：1998-07-01 00:00:00

abstract：:Three major subtypes of glutamate receptors that are coupled to cation channels--N-methyl-D-aspartate (NMDA), kainate, and alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptors--are known as ionotropic receptors in the mammalian CNS. Recently, an additional subtype that is coupled to GTP binding protei...

journal_title：Journal of neurochemistry

authors： Itano Y,Murayama T,Kitamura Y,Nomura Y

更新日期：1992-09-01 00:00:00