Abstract:
:Gene expression analysis of microRNA molecules is becoming increasingly important. In this study we assess the use of the mean expression value of all expressed microRNAs in a given sample as a normalization factor for microRNA real-time quantitative PCR data and compare its performance to the currently adopted approach. We demonstrate that the mean expression value outperforms the current normalization strategy in terms of better reduction of technical variation and more accurate appreciation of biological changes.
journal_name
Genome Bioljournal_title
Genome biologyauthors
Mestdagh P,Van Vlierberghe P,De Weer A,Muth D,Westermann F,Speleman F,Vandesompele Jdoi
10.1186/gb-2009-10-6-r64subject
Has Abstractpub_date
2009-01-01 00:00:00pages
R64issue
6eissn
1474-7596issn
1474-760Xpii
gb-2009-10-6-r64journal_volume
10pub_type
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