Abstract:
:We develop PIRCh-seq, a method which enables a comprehensive survey of chromatin-associated RNAs in a histone modification-specific manner. We identify hundreds of chromatin-associated RNAs in several cell types with substantially less contamination by nascent transcripts. Non-coding RNAs are found enriched on chromatin and are classified into functional groups based on the patterns of their association with specific histone modifications. We find single-stranded RNA bases are more chromatin-associated, and we discover hundreds of allele-specific RNA-chromatin interactions. These results provide a unique resource to globally study the functions of chromatin-associated lncRNAs and elucidate the basic mechanisms of chromatin-RNA interactions.
journal_name
Genome Bioljournal_title
Genome biologyauthors
Fang J,Ma Q,Chu C,Huang B,Li L,Cai P,Batista PJ,Tolentino KEM,Xu J,Li R,Du P,Qu K,Chang HYdoi
10.1186/s13059-019-1880-3subject
Has Abstractpub_date
2019-12-20 00:00:00pages
292issue
1eissn
1474-7596issn
1474-760Xpii
10.1186/s13059-019-1880-3journal_volume
20pub_type
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