Abstract:
:Peptide nucleic acids have a number of features that make them an ideal platform for the development of in vitro biological probes and tools. Unfortunately, their inability to pass through membranes has limited their in vivo application as diagnostic and therapeutic agents. Herein, we describe the development of cationic shell-cross-linked knedel-like (cSCK) nanoparticles as highly efficient vehicles for the delivery of PNAs into cells, either through electrostatic complexation with a PNA * ODN hybrid, or through a bioreductively cleavable disulfide linkage to a PNA. These delivery systems are better than the standard Lipofectamine/ODN-mediated method and much better than the Arg(g)-mediated method for PNA delivery in HeLa cells, showing lower toxicity and higher bioactivity. The cSCKs were also found to facilitate both endocytosis and endosomal release of the PNAs, while themselves remaining trapped in the endosomes.
journal_name
Mol Pharmjournal_title
Molecular pharmaceuticsauthors
Fang H,Zhang K,Shen G,Wooley KL,Taylor JSdoi
10.1021/mp800199wsubject
Has Abstractpub_date
2009-03-01 00:00:00pages
615-26issue
2eissn
1543-8384issn
1543-8392journal_volume
6pub_type
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