Abstract:
:RNA interference appears to have a great potential not only as an in vitro target validation, but also as a novel therapeutic strategy based on the highly specific and efficient silencing of a target gene. We hypothesize that MMP-9 siRNA can be effective as an MMP-9 protein inhibitor in a rat focal ischemia model. Male Sprague-Dawley rats (156) were subjected to 2 h of middle cerebral artery occlusion (by using the suture insertion method) followed by 24 h of reperfusion. In the treatment group, 5 microl MMP-9 siRNA was administrated by intracerebroventricular injection within 60 min after 2 h of focal ischemia. The siRNA transfection was demonstrated by fluorescence conjugated siRNA. Treatment with MMP-9 siRNA produced a significant reduction in the cerebral infarction volume, brain water content, mortality rate and accompanying neurological deficits. The followings were recorded: Evan's blue and IgG extravasation were reduced; the expression of MMP-9 mRNA and protein were significantly silenced; and immunohistochemistry and Western blot analysis revealed that the expression of MMP-9 and VEGF were reduced while occludin and collagen-IV were up-regulated in brain tissues. Our findings provide evidence that a liposomal formulation of siRNA might be used in vivo to silence the MMP-9 gene and could potentially serve as an important therapeutic alternative in patients with cerebral ischemia.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Hu Q,Chen C,Yan J,Yang X,Shi X,Zhao J,Lei J,Yang L,Wang K,Chen L,Huang H,Han J,Zhang JH,Zhou Cdoi
10.1016/j.expneurol.2008.11.007subject
Has Abstractpub_date
2009-03-01 00:00:00pages
35-46issue
1eissn
0014-4886issn
1090-2430pii
S0014-4886(08)00430-5journal_volume
216pub_type
杂志文章abstract::Alcohol intake is one of the important lifestyle factors for the risk of insulin resistance and type 2 diabetes. Acetaldehyde, the major ethanol metabolite which is far more reactive than ethanol, has been postulated to participate in alcohol-induced tissue injury although its direct impact on insulin signaling is unc...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2006.08.002
更新日期:2007-01-01 00:00:00
abstract::Acute exposure of peripheral axons to the free radical Nitric Oxide (NO) may trigger conduction block and, if prolonged, Wallerian degeneration. It was hypothesized that this neurotoxic effect of NO may be due primarily to energy restriction by inhibition of mitochondrial respiration. We compared the neurotoxic effect...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2008.03.022
更新日期:2008-07-01 00:00:00
abstract::Oxidative stress can contribute to many neurological disease processes. Because many events known to involve oxidative stress (infection, hemorrhage, brain trauma) are accompanied by hydrocephalus, the present study sought to evaluate the relationship between oxidative stress and the progression of hydrocephalus. Assa...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1998.6969
更新日期:1999-01-01 00:00:00
abstract::The expression of tyrosine hydroxylase (TH) was studied in adult mouse dorsal root ganglia (DRGs) and spinal cord by means of immunohistochemistry and in situ hybridization. TH immunoreactivity and TH mRNA were present in 10-15% of lumbar DRG neurons, in most cases being small/medium-sized. Only very few of these neur...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2006.01.023
更新日期:2006-07-01 00:00:00
abstract::Cortical spreading depression (CSD) is associated with various short- and long-term physiological and neurochemical changes and has been shown to confer an increased susceptibility to accompanying ischemic injury or provide protection against a subsequent experimental ischemia. Nitric oxide is involved in the processe...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1999.7218
更新日期:1999-12-01 00:00:00
abstract::With the use of four independent cell markers and Brd-U birthdating we have charted the earliest stages of neuronal differentiation and migration in the developing rat central nervous system, including the cortex, spinal cord, and retina. One of the markers, the monoclonal antibody 2G12, labeled a large subpopulation ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1995.1031
更新日期:1995-07-01 00:00:00
abstract::The antiretroviral toxic neuropathy, a distal sensory polyneuropathy associated with antiretroviral treatment, is a frequently occurring neurological complication during treatment of patients with AIDS and often leads to discontinuation of antiretroviral therapy. The mechanisms by which antiretroviral drugs contribute...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2015.02.036
更新日期:2015-05-01 00:00:00
abstract::We conducted a quantitative study of the trochlear motoneurons and the myotube clusters in the corresponding superior oblique muscle of the Japanese quail, before, during, and after the period of normally occurring motoneuron degeneration. A ratio of approximately 1:1 between myotube clusters and neurons was observed ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(85)90063-9
更新日期:1985-10-01 00:00:00
abstract::We have previously demonstrated that systemic administration of testosterone propionate (TP) to adult hamsters accelerates the rate of facial nerve regeneration following crush axotomy of the facial nerve at its exit from the stylomastoid foramen. In this study, we utilized flutamide, a potent nonsteroidal antiandroge...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1995.1016
更新日期:1995-06-01 00:00:00
abstract::Rat cortical explants were cultured in the presence or absence of the mitotic inhibitor cytosine arabinoside to determine whether or not it affects the composition of the nonneuronal cell population within the outgrowth zone. Ultrastructural morphometric analysis of the incidence of fibroblasts, fibrous astrocytes, an...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(86)90092-0
更新日期:1986-05-01 00:00:00
abstract::Development of outcome measures to assess ongoing nerve regeneration in the living animal that can be translated to human can provide extremely useful tools for monitoring the effects of therapeutic interventions to promote nerve regeneration. Diffusion tensor imaging (DTI), a magnetic resonance based technique, provi...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2009.10.012
更新日期:2010-05-01 00:00:00
abstract::Ubiquitin (Ub) C-terminal hydrolase L1 (UCHL1) is a multifunctional protein that is expressed in neurons throughout brain at high levels. UCHL1 deletion is associated with axonal degeneration, progressive sensory motor ataxia, and premature death in mice. UCHL1 has been hypothesized to play a role in the pathogenesis ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2020.113524
更新日期:2021-02-01 00:00:00
abstract::The afferent pathway involved in initiation of audiogenic seizures in the genetically epilepsy-prone rat was investigated by bilateral microinfusion of the excitant amino acid antagonist 2-amino-7-phosphonoheptanoate into the major brain stem and subcortical nuclei of the auditory system. This antagonist has been show...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(88)90184-7
更新日期:1988-03-01 00:00:00
abstract::Contusive spinal cord injury (SCI) results in the formation of a chronic lesion cavity surrounded by a rim of spared fibers. Tissue bridges containing axons extend from the spared rim into the cavity dividing it into chambers. Whether descending axons can grow into these trabeculae or whether fibers within the trabecu...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.2001.7734
更新日期:2001-09-01 00:00:00
abstract::Multiple system atrophy (MSA) is a rare neurodegenerative disease of undetermined cause manifesting with progressive autonomic failure (AF), cerebellar ataxia and parkinsonism due to neuronal loss in multiple brain areas associated with (oligodendro)glial cytoplasmic alpha-synuclein (alpha SYN) inclusions (GCIs). Usin...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2010.05.008
更新日期:2010-08-01 00:00:00
abstract::Although ciliary neurotrophic factor (CNTF) has been shown to promote the survival of magnocellular neurons when applied exogenously to explants of the paraventricular and supraoptic nuclei (SON) in vitro, little is known regarding its expression or regulation in the adult magnocellular neurosecretory system (MNS) fol...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2005.09.009
更新日期:2006-01-01 00:00:00
abstract::Pluripotent neural stem cells (NSCs) have been used as replacement cells in a variety of neurological disease models. Among the many different NSCs that have been used to date, most robust results have been obtained with the immortalized neural stem cell line (C17.2) isolated from postnatal cerebellum. However, it is ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2004.07.011
更新日期:2005-08-01 00:00:00
abstract::The effects of unilateral intrahippocampal injection of kainic acid, a potent neuroexcitant and neurotoxin, on subsequent susceptibility to kindling of the contralateral hippocampus or contralateral amygdala were investigated in albino rats. At the chosen doses (0.20 to 1.25 micrograms dissolved in physiologic saline)...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(87)90087-2
更新日期:1987-08-01 00:00:00
abstract::Thyroid hormones and spermidine, a motor neuron trophic polyamine (PA), have been shown to enhance peripheral motor nerve regeneration; however, the mechanism by which these treatment modalities exert their effect is unknown. Similarities in treatment outcome suggest that these molecules may be working via a common me...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2004.05.024
更新日期:2004-09-01 00:00:00
abstract::Successful clinical application of fMRI tasks requires reliable knowledge about the brain structures mapped by the task. With memory fMRI, diverging evidence exists concerning the location of major signal sources as well as hippocampal contributions. To clarify these issues, we investigated a frequently applied memory...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2008.05.019
更新日期:2008-09-01 00:00:00
abstract::l-DOPA therapy in Parkinson's disease often results in side effects such as l-DOPA-induced dyskinesia (LID). Our previous studies demonstrated that defective desensitization of dopamine receptors caused by decreased expression of G protein-coupled receptor kinases (GRKs) plays a role. Overexpression of GRK6, the isofo...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2015.02.008
更新日期:2015-04-01 00:00:00
abstract::Previously, our lab reported the isolation of patient-specific neurosphere-forming progenitor lines from human adult olfactory epithelium from cadavers as well as patients undergoing nasal sinus surgery. RT-PCR and ELISA demonstrated that the neurosphere-forming cells (NSFCs) produced BDNF. Since rubrospinal tract (RS...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2005.01.021
更新日期:2005-07-01 00:00:00
abstract::Neurotrophic factors as candidates for ALS therapeutics have previously been studied in the context of attempts to slow disease progression. For a variety of reasons, clinical trials of neurotrophic factors have failed to show efficacy in ALS patients. Previous studies in Parkinson's Disease (PD) models have shown pro...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2019.113091
更新日期:2020-01-01 00:00:00
abstract::The present study demonstrates that the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) causes significantly greater reductions in striatal dopamine levels in C57/bl mice than in CD-1 mice, thus confirming a greater sensitivity of the C57/bl mice to MPTP. To determine the possible reasons for this diffe...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1994.1058
更新日期:1994-04-01 00:00:00
abstract::The aminergic and cholinergic fibers innervating the hippocampus reach their target regions via the dorsal (dorsal fornix, fimbria, and cingulum) and ventral routes. The plasticity of this innervation after lesions of the dorsal pathway was investigated in the septal, medial, and temporal regions of the adult rat hipp...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(87)90053-7
更新日期:1987-05-01 00:00:00
abstract::Cereport (RMP-7) enhances delivery of chemotherapeutics into brain tumors by increasing the permeability of the glioma vasculature (i.e. , the blood-brain tumor barrier; BBTB). Its effect on brain tumors has consistently been more robust than that on normal brain. The present experiments tested the hypothesis that the...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1999.7247
更新日期:2000-01-01 00:00:00
abstract::Various limbic system structures have been implicated in processing noxious information. One such structure is the anterior cingulate cortex (ACC), a region that is thought to modulate higher order processing of noxious input related to the affective/motivational component of pain. The present experiment examined the ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2004.04.003
更新日期:2004-07-01 00:00:00
abstract::We have recently shown that tanycytes present in the median eminence (ME) constitute a preferential support for the regeneration of lesioned neurohypophysial oxytocinergic and vasopressinergic axons. However, although tanycytes are particularly abundant in the ME, they are also present along the third ventricle wall. ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1998.6784
更新日期:1998-05-01 00:00:00
abstract::The beta-Amyloid peptide (A beta) is hypothesized to mediate the neurodegeneration seen in Alzheimer's disease. Recently, we proposed a new hypothesis to explain the toxicity of A beta based on the free-radical generating capacity of A beta. We have recently demonstrated using electron paramagnetic resonance (EPR) spe...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(95)90041-1
更新日期:1995-02-01 00:00:00
abstract::The regulation of perinatal glia limitans (GL) reformation by interleukin-1beta (IL-1beta) following prenatal neural trauma in the mouse was studied in lesioned fetal mice by immunocytochemistry and computer-assisted image analysis for presence and distribution of astrocytes and IL-1beta immunoreactivity (ir). Astrocy...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1997.6470
更新日期:1997-06-01 00:00:00