Abstract:
:Pluripotent neural stem cells (NSCs) have been used as replacement cells in a variety of neurological disease models. Among the many different NSCs that have been used to date, most robust results have been obtained with the immortalized neural stem cell line (C17.2) isolated from postnatal cerebellum. However, it is unclear if other NSCs isolated from different brain regions are similar in their potency as replacement therapies. To assess the properties of NSC-like C17.2 cells, we compared the properties of these cells with those reported for other NSC populations identified by a variety of different investigators using biological assays, microarray analysis, RT-PCR, and immunocytochemistry. We show that C17.2 cells differ significantly from other NSCs and cerebellar granule cell precursors, from which they were derived. In particular, they secrete additional growth factors and cytokines, express markers that distinguish them from other progenitor populations, and do not maintain karyotypic stability. Our results provide a caution on extrapolating results from C17.2 to other nonimmortalized stem cell populations and provide an explanation for some of the dramatic effects that are seen with C17.2 transplants but not with other cells. We suggest that, while C17.2 cells can illustrate many fundamental aspects of neural biology and are useful in their own right, their unique properties cannot be generalized.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Mi R,Luo Y,Cai J,Limke TL,Rao MS,Höke Adoi
10.1016/j.expneurol.2004.07.011keywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
301-19issue
2eissn
0014-4886issn
1090-2430pii
S0014-4886(04)00297-3journal_volume
194pub_type
杂志文章abstract::The tachykinin neuropeptides acting at NK3 receptors affect mean arterial pressure (MAP) through both neuroendocrine and neural mechanisms. NK3 receptors are found in brainstem nuclei that mediate the baroreflex, but the effects of NK3 receptor stimulation on baroreflex function are unknown. The present study tests th...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2004.12.011
更新日期:2005-05-01 00:00:00
abstract::The aim of this study was to examine whether acute nerve compression damages an axonal transport system based on microtubules and how the fibers recover after the compression. A 5-mm segment of the tibial nerve of male wistar rat was compressed with a specially designed clip. Functional recovery was assessed using Tib...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1997.6601
更新日期:1997-10-01 00:00:00
abstract::The ventrolateral orbital cortex (VLO) is part of an endogenous analgesic system consisting of an ascending pathway from the spinal cord to VLO via the thalamic nucleus submedius (Sm) and a descending pathway to the spinal cord relaying in the periaqueductal gray (PAG). This study examines whether activation of D(1)-l...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2008.09.018
更新日期:2009-01-01 00:00:00
abstract::One of the major histopathological hallmarks of Alzheimer's disease (AD) is redundant senile plaques mainly composed of beta-amyloid (Abeta) aggregates. Alternative cleavage of the amyloid precursor protein (APP), occurring in both normal and AD subjects, results in the generation and secretion of soluble APP (sAPP) a...
journal_title:Experimental neurology
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1016/s0014-4886(03)00027-x
更新日期:2003-09-01 00:00:00
abstract::The role of capsaicin-sensitive afferents in neurogenic voiding dysfunction was studied in chronic spinal cord injured rats (SCI). Cystometry and external urethral sphincter (EUS) electromyography were performed on 2 consecutive days after induction of urethane anesthesia in SCI rats 6-8 weeks after spinal cord injury...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2004.02.014
更新日期:2004-06-01 00:00:00
abstract::Multiple cellular and molecular interactions are required for the differentiation and development of central neurons. For example, neural activity may modulate trophic function. In the developing cerebellum, establishment of functional excitatory synaptic connections coincides with the expression of NGF and its recept...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1993.1018
更新日期:1993-02-01 00:00:00
abstract::Both alpha- and beta-adrenergic receptors (ARs) are involved in the facilitation of the monosynaptic jaw-closing reflex in the trigeminal motor nucleus (MoV) caused by norepinephrine (NE). The amplitude of muscle spindle afferent-evoked EPSPs in masseter motor neurons is 65% greater when noradrenergic axons to the mot...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(91)90038-e
更新日期:1991-11-01 00:00:00
abstract::To test the hypothesis that prepubertal exposure of LHRH neurons to a source of catecholamines and neuropeptides accelerates the onset of puberty, we examined the effects of autologous adrenal transplantation into the base of the third ventricle of the brain in juvenile female rhesus monkeys at 11-13 months of age. Th...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1996.0127
更新日期:1996-08-01 00:00:00
abstract::Urethane-induced cortical slow wave activity (SWA) spreads into the basal ganglia in dopamine (DA)-depleted rat models of Parkinson's disease (PD). During physiological sleep, SWA is powerfully expressed at the beginning of night and progressively reduced during sleep-time reflecting the sleep need. However, its under...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2017.12.004
更新日期:2018-03-01 00:00:00
abstract::Subarachnoid hemorrhage (SAH) is the most devastating form of stroke. Reducing neuronal apoptosis is an important countermeasure against early brain injury (EBI) after SAH. Recent evidence indicates that OX40-OX40L coupling is critical for cell survival and proliferation. Current study was performed to detect the role...
journal_title:Experimental neurology
pub_type: 杂志文章,评审
doi:10.1016/j.expneurol.2020.113179
更新日期:2020-04-01 00:00:00
abstract::A "neuroplastic" hypothesis proposes that changes in neuronal structural plasticity may underlie the aetiology of depression and the action of antidepressants. The medial prefrontal cortex (mPFC) is affected by this disorder and shows an intense expression of the polysialylated form of the neural cell adhesion molecul...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2008.07.018
更新日期:2008-11-01 00:00:00
abstract::Dorsal root ganglia (DRG) neuronopathy was induced in rats by chronic treatment (2 mg/kg twice a week for nine injections) with the antineoplastic drug cisplatin. Morphological alterations and changes in peptide [calcitonin gene-related peptide (CGRP), substance P, galanin (Gal), and somatostatin] concentration were s...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1996.0050
更新日期:1996-03-01 00:00:00
abstract::In chronically implanted rats, we examined the respiratory EMG activity of the two parts of the diaphragm, costal and crural, during sleep and wakefulness. Their activity was compared and contrasted with that of the EMG activity of the cricothyroid muscle. Whether in wakefulness, while grooming and drinking, or in non...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(85)90051-2
更新日期:1985-10-01 00:00:00
abstract::Cyclic nucleotides are thought to act as second messengers of neurotransmission inside central neurons, and cyclic guanosine monophosphate (cGMP) has been postulated to act as a messenger for muscarinic, cholinergic transmission. Nonetheless, the action of cGMP has not yet been established in identified cortical neuro...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(86)90054-3
更新日期:1986-03-01 00:00:00
abstract::Neurons from cerebral neocortex and hippocampus exhibit a striking difference in vulnerability to transient global ischemia. In order to study the contribution of neuronal connections and neuron-glia interactions to this variation in neuronal vulnerability, we used hippocampal and neocortical cultures submitted to var...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1997.6728
更新日期:1998-03-01 00:00:00
abstract::Adaptive responses in glutamate and opioid receptor systems in limbic circuits are emerging as a critical component of the neural plasticity induced by chronic use of abused substances. The present commentary reviews findings from neuroanatomical studies, with superior spatial resolution, that support a cellular basis...
journal_title:Experimental neurology
pub_type: 评论,杂志文章
doi:10.1016/j.expneurol.2011.03.016
更新日期:2011-06-01 00:00:00
abstract::Spinal ischemia is a frequent cause of paralysis. Here we explore the biological basis of ischemic preconditioning (IPC), the phenomenon in which a brief period of ischemia can confer protection against subsequent longer and normally injurious ischemia, to identify mediators of endogenous neuroprotection. Using microa...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2003.09.007
更新日期:2004-01-01 00:00:00
abstract::Functional regeneration of brainstem-spinal pathways occurs in the developing chick when the spinal cord is severed prior to embryonic day (E) 13. Functional spinal cord regeneration is not observed in animals injured after E13. This developmental transition from a permissive to a restrictive repair period may be due ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.2000.7496
更新日期:2000-11-01 00:00:00
abstract::Wistar rats (n = 58) were injected subcutaneously during the lactation period with fluoxetine (5, 10, 20 or 40 mg/kg/day) and cortical spreading depression (SD) was recorded immediately after weaning (25-30 days of life). An additional group (10 mg/kg; n = 8) was SD-recorded at 60-70 days. As compared to the saline-in...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2006.02.014
更新日期:2006-08-01 00:00:00
abstract::Ketamine has been reported to exert a prophylactic effect against stress-induced depressive-like behavior by modulating the guanosine-based purinergic system. However, the molecular pathways underlying its prophylactic effect and whether guanosine also elicits a similar effect remain to be determined. Here, we investi...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2020.113459
更新日期:2020-12-01 00:00:00
abstract::Spinal cord injury (SCI) initiates biochemical events that lead to an increase in extracellular excitatory amino acid concentrations, resulting in glutamate receptor-mediated excitotoxic events. These receptors include the three groups of metabotropic glutamate receptors (mGluRs). Group I mGluR activation can initiate...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.2001.7721
更新日期:2001-08-01 00:00:00
abstract::This study examined the effect of experimental diabetes on the anterograde and retrograde axonal transport of phosphofructokinase activity. Rats with streptozotocin-induced diabetes of 4 weeks duration showed phosphofructokinase activity accumulation deficits both proximal (53% and 65% in two separate experiments) and...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(87)90180-4
更新日期:1987-04-01 00:00:00
abstract::The role of central noradrenergic neurons in kindled seizures was assessed by comparison of alpha 2- and beta-adrenoceptor binding in the cerebral cortex from kindled and control rats. To minimize handling, which may modify kindling-induced changes in binding, the kindling protocol involved stimulation of the amygdala...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(85)90044-5
更新日期:1985-10-01 00:00:00
abstract::Crush of sciatic nerve in rabbits supported by morphologic and electrophysiologic data was used to evaluate the effects of ACTH4-10 on nerve fiber regeneration. Treated animals showed a statistically significant higher regeneration rate than did control rabbits. Fiber density and mean diameters of myelinated nerve fib...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(88)90162-8
更新日期:1988-02-01 00:00:00
abstract::The possible pain inhibitory effects of periaqueductal gray (PAG) stimulation were investigated in cats anesthetized with Nembutal and immobilized with Flaxedil. Unitary responses evoked by electrical stimulation of the upper canine dentine and by cutaneous facial noxious and nonnoxious stimuli were recorded extracell...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(83)90155-3
更新日期:1983-07-01 00:00:00
abstract::Chewing rhythms are set by a putative central pattern generator whose output is influenced by sensory feedback. In this study we assessed how an altered feedback imposed by changing the hardness of a gum bolus modifies the timing of chewing, the maximal gape, and the activity in the masseter muscle on the chewing side...
journal_title:Experimental neurology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0014-4886(86)90292-x
更新日期:1986-06-01 00:00:00
abstract::Rats were injected with capsaicin or solvent on the 2nd day of life. Nine weeks later both animal groups were further subdivided: one was untreated and the other group underwent surgery consisting in either (a) removal of one sternohyoid superior muscle or (b) a sham operation. The sternohyoid superior muscles were re...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(85)90124-4
更新日期:1985-04-01 00:00:00
abstract::Neuronal migration was investigated in rodent cerebellum in vitro and in vivo. Time-lapse video recording showed that cultured neurons migrated on laminin by first extending neurites that formed contacts with other neurons. This was followed by movement of the cell nucleus inside the preformed process. No guidance cue...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(92)90119-b
更新日期:1992-08-01 00:00:00
abstract::In a model of Parkinson's disease (PD), amphetamine, a dopamine (DA)-releasing drug, fails to induce ipsilateral drug rotations in a proportion of rats with complete unilateral 6-hydroxydopamine (6-OHDA) lesions of the medial forebrain bundle and DA neurons of the substantia nigra. To investigate this phenomenon, indi...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.2001.7823
更新日期:2001-12-01 00:00:00
abstract::Although aluminum (Al) contributes to a variety of cognitive dysfunctions and mental diseases, the underlying mechanisms of Al interactions with the nervous system are still unknown. We have studied the action of Al on synaptic transmission and long-term potentiation (LTP) by performing electrophysiological recordings...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1995.1038
更新日期:1995-07-01 00:00:00