Concentration of bone marrow aspirate for osteogenic repair using simple centrifugal methods.

Abstract:

:Bone marrow transplantation can lead to osteogenic repair of intractable bone conditions. To achieve optimal clinical results, it is necessary to transplant as many cells with osteogenetic potential as possible. However, approaches involving special equipment and reagents for the extraction and purification of cells are expensive, and the complicated procedures involved are a hindrance to widespread acceptance of bone marrow transplantation for osteogenic repair. To standardize bone marrow transplantation for bone regeneration, a simple, safe, clean, and low-cost system is required. We describe an easy-to-use method using a conventional manual blood bag centrifugation technique traditionally used for extracting buffy coats, for concentration of cells from bone marrow aspirates (BMAs) to obtain osteogenic progenitors.

journal_name

Acta Orthop

journal_title

Acta orthopaedica

authors

Sakai S,Mishima H,Ishii T,Akaogi H,Yoshioka T,Uemura T,Ochiai N

doi

10.1080/17453670710015382

subject

Has Abstract

pub_date

2008-06-01 00:00:00

pages

445-8

issue

3

eissn

1745-3674

issn

1745-3682

pii

794950816

journal_volume

79

pub_type

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