当前位置： SCI文献检索 > JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY期刊下所有文献 > Globular adiponectin but not full-length adiponectin induces increased procoagulability in human endothelial cells.

Globular adiponectin but not full-length adiponectin induces increased procoagulability in human endothelial cells.

Abstract：

:Adiponectin (APN), a recently discovered adipocytokine, is present in human serum in a full length (fAPN) and a globular form (gAPN). gAPN is a proteolytic cleavage product of fAPN and seems to show independent biological activities compared to the properties of fAPN. The influence of gAPN and fAPN on procoagulability of cells is still unknown. This study examined the effect of gAPN and fAPN on the expression of tissue factor (TF), the initiator of the extrinsic coagulation system, in human umbilical vein endothelial cells (HUVECs). TF activity was measured by a chromogenic assay, TF mRNA by real-time PCR and TF protein by western blot. We found TF activity to be increased after activation by gAPN (3 microg/mL) compared to a non-stimulated control (169.0+/-19.23 U versus 501.9+/-38.95 U, p<0.001). Furthermore, TF mRNA and TF protein was increased dose-dependently after gAPN stimulation. The gAPN-induced rise of TF activity and TF mRNA was significantly reduced by inhibition of the MAP kinases ERK1/2, p38 and JNK. Contrary to gAPN, stimulation with fAPN did not lead to these procoagulant effects. In conclusion, gAPN increased TF transcription, expression and activity in HUVECs. Therefore, our data support the theory that gAPN but not fAPN supports the cellular procoagulability via TF upregulation.

journal_name

J Mol Cell Cardiol

journal_title

Journal of molecular and cellular cardiology

authors

Bobbert P,Antoniak S,Schultheiss HP,Rauch U

doi

10.1016/j.yjmcc.2007.10.018

subject

Has Abstract

pub_date

2008-02-01 00:00:00

pages

388-94

issue

2

eissn

0022-2828

issn

1095-8584

pii

S0022-2828(07)01284-9

journal_volume

44

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY文献大全
  • Effect of isoproterenol on myocardial mechanical function and cyclic AMP content in the fetal rabbit.

    abstract：:The effect of isoproterenol on mechanical function was studied in the isolated arterially perfused heart of the fetal (21st and 28th day of gestation) and newborn rabbits. The inotropic effect of isoproterenol in the fetus was less than in the newborn. In contrast, myocardial cyclic AMP levels after isoproterenol infu...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(87)80557-6

    authors： Okuda H,Nakanishi T,Nakazawa M,Takao A

    更新日期：1987-02-01 00:00:00

  • Evidence for a sustained effectiveness of sodium-channel activators in failing human myocardium.

    abstract：:Elevation of cytosolic sodium is thought to be correlated with an increase in force of contraction due to an activation of sodium-calcium exchange. We investigated the inotropic response mediated by the new sodium-channel activator BDF 9148 (0.01-100 mumol/l) on failing human myocardium. Force of contraction was studi...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(91)90170-q

    authors： Schwinger RH,Böhm M,Mittmann C,La Rosée K,Erdmann E

    更新日期：1991-04-01 00:00:00

  • Effect of ovariectomy and estrogen replacement on cardiovascular disease in heart failure-prone SHHF/Mcc- fa cp rats.

    abstract：:The importance of endogenous and exogenous estrogen levels to the development of cardiovascular disease in women in controversial. The purpose of our study was to examine the effect of estrogen on the development of hypertension, cardiac hypertrophy, ventricular function, and gene expression for atrial natriuretic pep...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1999.0985

    authors： Sharkey LC,Holycross BJ,Park S,Shiry LJ,Hoepf TM,McCune SA,Radin MJ

    更新日期：1999-08-01 00:00:00

  • A biochemical method for the quantitation of myocardial scarring after experimental coronary artery occlusion.

    abstract：:The histologic examination and planimetric evaluation of tissue slices has been so far the only available technique for studying the extent of experimental myocardial infarcts in long-term studies; however, this approach is rather time-consuming when the sample size is large. This study describes a new biochemical met...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(86)80410-2

    authors： Chiariello M,Ambrosio G,Cappelli-Bigazzi M,Perrone-Filardi P,Brigante F,Sifola C

    更新日期：1986-03-01 00:00:00

  • Proto-oncogene expression in the isolated working rat heart: combination of pressure and volume overload with norepinephrine.

    abstract：:We have studied the effects of pressure and volume overload as well as of norepinephrine (NE) alone and in combination on the expression of the proto-oncogenes c-fos and c-myc in isolated perfused working rat hearts. Using specific cDNA clones, the mRNAs of c-fos and c-myc were measured by Northern blots and quantifie...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(08)80045-4

    authors： Kolbeck-Rühmkorff C,Zimmer HG

    更新日期：1995-01-01 00:00:00

  • Protection of energy status of hypoxic cardiomyocytes by mild acidosis.

    abstract：:The question was investigated whether mild acidosis can provide energetic protection on the hypoxic myocardial cell by mechanisms other than negative inotropy. The experimental model used was quiescent ventricular cardiomyocytes isolated from the adult rat. The extracellular pH (pHe) in modified hypoxic Tyrode's solut...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(92)91159-3

    authors： Koop A,Piper HM

    更新日期：1992-01-01 00:00:00

  • Contractile performance of adult ventricular rat cardiomyocytes is not directly jeopardized by NO/cGMP-dependent induction of pro-apoptotic pathways.

    abstract：:The activation of NO/cGMP pathways can induce pro-apoptotic pathways in cardiomyocytes although only a small number of cardiomyocytes fulfill the criteria of apoptosis. The same pathways reduce the contractile performance of cardiomyocytes. In the present study, we tested the hypothesis that exposure of cells to NO/cG...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2006.10.012

    authors： Tastan H,Abdallah Y,Euler G,Piper HM,Schlüter KD

    更新日期：2007-02-01 00:00:00

  • Restoration of mechanical and energetic function in failing aortic-banded rat hearts by gene transfer of calcium cycling proteins.

    abstract：:The aim of this study was to examine whether short- and long-term gene transfer of Ca(2+) handling proteins restore left ventricular (LV) mechanoenergetics in aortic banding-induced failing hearts. Aortic-banded rats received recombinant adenoviruses carrying sarcoplasmic reticulum Ca(2+)-ATPase (SERCA2a) (Banding+SER...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2007.01.003

    authors： Sakata S,Lebeche D,Sakata N,Sakata Y,Chemaly ER,Liang LF,Tsuji T,Takewa Y,del Monte F,Peluso R,Zsebo K,Jeong D,Park WJ,Kawase Y,Hajjar RJ

    更新日期：2007-04-01 00:00:00

  • Chemokine expression in myocardial ischemia: MIP-2 dependent MCP-1 expression protects cardiomyocytes from cell death.

    abstract：:Chemokines are small molecular weight proteins that play important roles in inflammation. Originally described as chemotactic cytokines, chemokines stimulate the influx of leukocytes into specific tissue compartments. These molecules also modulate gene expression in both infiltrating and resident cells to mediate a va...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.2001.1503

    authors： Tarzami ST,Cheng R,Miao W,Kitsis RN,Berman JW

    更新日期：2002-02-01 00:00:00

  • Inhibition of Pyk2 and Src activity improves Cx43 gap junction intercellular communication.

    abstract：:Identification of proteins that interact with Cx43 has been instrumental in the understanding of gap junction (GJ) regulation. An in vitro phosphorylation screen identified that Protein tyrosine kinase 2 beta (Pyk2) phosphorylated purified Cx43CT and this led us to characterize the impact of this phosphorylation on Cx...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2020.09.004

    authors： Zheng L,Trease AJ,Katsurada K,Spagnol G,Li H,Shi W,Duan B,Patel KP,Sorgen PL

    更新日期：2020-12-01 00:00:00

  • Energy metabolism in the perfused, arrested rabbit heart.

    abstract：:Energy metabolism of quiescent cardiac muscle was studied in the isolated rabbit heart preparation perfused at constant pressure by the Langendorff technique. Oxygen consumption (MVo2), coronary flow rate (CFR) and the steady state concentrations of high energy phosphate compounds were determined in hearts rendered as...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(89)90863-8

    authors： Kotsanas G,Gibbs CL,Wendt IR

    更新日期：1989-02-01 00:00:00

  • Effect of ionic strength on length-dependent Ca(2+) activation in skinned cardiac muscle.

    abstract：:The length-dependence of myofilament Ca(2+) sensitivity is considered to be an important component of the steep force-length relationship in cardiac muscle (Frank-Starling relation). Recent studies suggest that Ca(2+) sensitivity is a function of the number of strong-binding cross-bridge interactions formed at a given...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1999.1043

    authors： Smith SH,Fuchs F

    更新日期：1999-12-01 00:00:00

  • Protein metabolism of the heart.

    abstract：:The heart contains many cell types; mechanical work is done by cardiomyocytes which do not divide but are terminally differentiated and capable of continuous protein synthesis and degradation. The steps in protein synthesis are (1) transport of amino acids into heart cells by a variety of cell-membrane carriers, (2) A...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/s0022-2828(84)80711-7

    authors： Gevers W

    更新日期：1984-01-01 00:00:00

  • Ablation of biglycan attenuates cardiac hypertrophy and fibrosis after left ventricular pressure overload.

    abstract：AIMS:Biglycan, a small leucine-rich proteoglycan, has been shown to play an important role in stabilizing fibrotic scars after experimental myocardial infarction. However, the role of biglycan in the development and regression of cardiomyocyte hypertrophy and fibrosis during cardiac pressure overload and unloading rema...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2016.10.011

    authors： Beetz N,Rommel C,Schnick T,Neumann E,Lother A,Monroy-Ordonez EB,Zeeb M,Preissl S,Gilsbach R,Melchior-Becker A,Rylski B,Stoll M,Schaefer L,Beyersdorf F,Stiller B,Hein L

    更新日期：2016-12-01 00:00:00

  • S100A1 in cardiovascular health and disease: closing the gap between basic science and clinical therapy.

    abstract：:Calcium (Ca(2+)) signaling plays a major role in a wide range of physiological functions including control and regulation of cardiac and skeletal muscle performance and vascular tone. As all Ca(2+) signals require proteins to relay intracellular Ca(2+) oscillations downstream to different signaling networks, a specifi...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2009.06.003

    authors： Kraus C,Rohde D,Weidenhammer C,Qiu G,Pleger ST,Voelkers M,Boerries M,Remppis A,Katus HA,Most P

    更新日期：2009-10-01 00:00:00

  • MEK1/2-ERK1/2 mediates alpha1-adrenergic receptor-stimulated hypertrophy in adult rat ventricular myocytes.

    abstract：:We examined the relative roles of the mitogen-activated protein kinases (MAPK) in mediating the alpha1-adrenergic receptor (alpha1-AR) stimulated hypertrophic phenotype in adult rat ventricular myocytes (ARVM). Norepinephrine (NE; 1 microM) in the presence of the beta -AR antagonist propranolol (Pro; 2 microM) caused ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.2001.1348

    authors： Xiao L,Pimental DR,Amin JK,Singh K,Sawyer DB,Colucci WS

    更新日期：2001-04-01 00:00:00

  • Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion.

    abstract：:Since ascorbate is unnecessary for cell growth and survival, cardiac fibroblasts are routinely cultured without it. However, ascorbate is necessary for optimal collagen synthesis, so we hypothesized that its presence would influence cell phenotype. Cardiac fibroblasts cultured without ascorbate had increased intracell...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2017.09.005

    authors： Cowling RT,Park JI,Sotimehin AE,Greenberg BH

    更新日期：2017-12-01 00:00:00

  • Comparison of different iron chelators as protective agents against acute doxorubicin-induced cardiotoxicity.

    abstract：:Doxorubicin (Dox) is a widely used antineoplastic agent. Irreversible cardiomyopathy is a serious and dose-limiting side effect after chronic administration. The iron chelating bispiperazinedione ICRF-187 is currently the only drug which affords protection against Dox-induced cardiotoxicity. To compare the protective ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1994.1136

    authors： Voest EE,van Acker SA,van der Vijgh WJ,van Asbeck BS,Bast A

    更新日期：1994-09-01 00:00:00

  • Gender influences on sarcoplasmic reticulum Ca2+-handling in failing human myocardium.

    abstract：:Gender has recently been implicated as an important modulator of cardiovascular disease. However, it is not known how gender may specifically influence the Ca2+-handling deficits that characterize the depressed cardiac contractility of human heart failure. To elucidate the contributory role of gender to sarcoplasmic r...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.2001.1394

    authors： Dash R,Frank KF,Carr AN,Moravec CS,Kranias EG

    更新日期：2001-07-01 00:00:00

  • Regulation of mTOR and S6K1 activation by the nPKC isoforms, PKCepsilon and PKCdelta, in adult cardiac muscle cells.

    abstract：:Activation of both mTOR and its downstream target, S6K1 (p70 S6 kinase) have been implicated to affect cardiac hypertrophy. Our earlier work, in a feline model of 1-48 h pressure overload, demonstrated that mTOR/S6K1 activation occurred primarily through a PKC/c-Raf pathway. To further delineate the role of specific P...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2007.09.015

    authors： Moschella PC,Rao VU,McDermott PJ,Kuppuswamy D

    更新日期：2007-12-01 00:00:00

  • Influence of protein kinase phosphorylation on isolated sarcolemmal membranes.

    abstract：:A cardiac muscle sarcolemmal preparation, enriched in adenylate cyclase, Na+, K+ -ATPase, beta, muscarinic and ouabain receptors, also contained endogenous protein kinase activity. Phosphorylation of sarcolemmal membrane proteins by the endogenous protein kinase occurred mainly on 22 000 and 12 000 Mr proteins. To det...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(85)80124-3

    authors： Lee SW,Wallick ET,Schwartz A,Kranias EG

    更新日期：1985-11-01 00:00:00

  • Pyruvate attenuation of hypoxia damage in isolated working guinea-pig heart.

    abstract：:Function and various parameters of myocardial substrate and energy metabolism were measured in preload-controlled isolated working guinea-pig hearts perfused with normoxic (95% O2) and hypoxic (30 to 45% O2) Krebs-Henseleit buffers ([Ca2+] = 1.25 mM). Energy-yielding substrates were glucose, pyruvate, lactate, and fat...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(86)80905-1

    authors： Bünger R,Swindall B,Brodie D,Zdunek D,Stiegler H,Walter G

    更新日期：1986-04-01 00:00:00

  • Intracellular angiotensin II fusion protein alters AT1 receptor fusion protein distribution and activates CREB.

    abstract：:In recently published studies, we show that angiotensin II (AII) generated from an engineered rat angiotensinogen cDNA, and maintained intracellularly, is growth stimulatory for a rat hepatoma cell line. In the present study, we report that co-expression of AII fused to cyan fluorescent protein (ECFP/AII) and angioten...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2003.09.021

    authors： Cook JL,Re R,Alam J,Hart M,Zhang Z

    更新日期：2004-01-01 00:00:00

  • Antiplatelet monoclonal F(ab')2 antibody directed against the platelet GPIIb/IIIa receptor complex prevents coronary artery thrombosis in the canine heart.

    abstract：:Interactions between platelets with injured vascular endothelium contribute to thrombotic occlusion. A murine monoclonal antibody [7E3 F(ab')2] to the platelet GPIIb/IIIa receptor complex was used to inhibit platelet aggregation in an experimental model of coronary artery thrombosis. Prevention of thrombotic occlusion...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(89)90650-0

    authors： Mickelson JK,Simpson PJ,Lucchesi BR

    更新日期：1989-04-01 00:00:00

  • Relative abundance of alpha2 Na(+) pump isoform influences Na(+)-Ca(2+) exchanger currents and Ca(2+) transients in mouse ventricular myocytes.

    abstract：:In the mouse, genetic reduction in the Na(+), K(+)-ATPase alpha1 or alpha2 isoforms results in different functional phenotypes: heterozygous alpha2 isolated hearts are hypercontractile, whereas heterozygous alpha1 hearts are hypocontractile. We examined Na(+)/Ca(2+) exchange (NCX) currents in voltage clamped myocytes ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2005.03.023

    authors： Yamamoto T,Su Z,Moseley AE,Kadono T,Zhang J,Cougnon M,Li F,Lingrel JB,Barry WH

    更新日期：2005-07-01 00:00:00

  • Stem cell therapy enhances electrical viability in myocardial infarction.

    abstract：:Clinical studies suggest increased arrhythmia risk associated with cell therapy for myocardial infarction (MI); however, the underlying mechanisms are poorly understood. We hypothesize that the degree of electrical viability in the infarct and border zone associated with skeletal myoblast (SKMB) or mesenchymal stem ce...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2006.09.011

    authors： Mills WR,Mal N,Kiedrowski MJ,Unger R,Forudi F,Popovic ZB,Penn MS,Laurita KR

    更新日期：2007-02-01 00:00:00

  • An in vitro model for the assessment of stem cell fate following implantation within the infarct microenvironment identifies ISL-1 expression as the strongest predictor of c-Kit(+) cardiac progenitor cells' therapeutic potential.

    abstract：:Cell therapy has the potential to drastically improve clinical outcomes for the 1.45 million patients suffering from a myocardial infarction (MI) each year in the U.S. However, the limitations associated with this treatment - including poor engraftment, significant cell death and poor differentiation potential - have ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2015.09.007

    authors： Sullivan KE,Burns LJ,Black LD 3rd

    更新日期：2015-11-01 00:00:00

  • The myocardial content of fatty acids and phospholipids during the calcium paradox.

    abstract：:Readmission of Ca2+ after a short period of Ca2+-free perfusion results in a rapid and massive release of cytoplasmic proteins from the heart (calcium paradox). Maximal release rates of proteins are already reached within 2 min after Ca2+ repletion. The precise mechanism underlying the loss of cellular membrane integr...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(88)80119-6

    authors： van der Vusse GJ,van Bilsen M,Willemsen P,Reneman RS

    更新日期：1988-07-01 00:00:00

  • Calpain mediates cardiac troponin degradation and contractile dysfunction in atrial fibrillation.

    abstract：:The self-perpetuation of atrial fibrillation (AF) is associated with atrial remodeling, including the degradation of the myofibril structure (myolysis). Myolysis is related to AF-induced activation of cysteine proteases and underlies loss of contractile function. In this study, we investigated which proteases are invo...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2008.08.012

    authors： Ke L,Qi XY,Dijkhuis AJ,Chartier D,Nattel S,Henning RH,Kampinga HH,Brundel BJ

    更新日期：2008-11-01 00:00:00

  • The SCFFBXO3 ubiquitin E3 ligase regulates inflammation in atherosclerosis.

    abstract：:Inflammation is critical in the pathobiology of atherosclerosis. An essential player in the inflammatory process in atherosclerosis are macrophages that scavenge oxidatively modified low-density lipoproteins (OxLDL) deposited in the subendothelium of systemic arteries that secrete a myriad of pro-inflammatory mediator...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2018.11.006

    authors： Chandra D,Londino J,Alexander S,Bednash JS,Zhang Y,Friedlander RM,Daskivich G,Carlisle DL,Lariviere WR,Nakassa ACI,Ross M,St Croix C,Nyunoya T,Sciurba F,Chen B,Mallampalli RK

    更新日期：2019-01-01 00:00:00