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Interactions with filamin A stimulate surface expression of large-conductance Ca2+-activated K+ channels in the absence of direct actin binding.

Abstract：

:Large-conductance Ca(2+)-activated K(+) (BK(Ca)) channels play an important role in the regulation of cell physiology in a wide variety of excitable and nonexcitable tissues. Filamin A is a conserved and ubiquitous actin-binding protein that forms perpendicular actin cross-links and contributes to changes in cell shape, stiffness, and motility. A variety of membrane proteins bind to filamin A, which regulates their trafficking in and out of the plasma membrane. Filamin A is therefore believed to couple membrane dynamics with those of the underlying cytoskeleton. Filamin A was identified in a yeast two-hybrid screen of a neuronal transcriptome using a subunit of BK(Ca) channels as bait, and the interaction was confirmed by a variety of biochemical assays in native neuronal cells and in human embryonic kidney 293T cells expressing BK(Ca) channels. BK(Ca) channels do not traffic to the plasma membrane in M2 melanoma cells, which lack filamin A, but normal trafficking is seen in A7 cells, which express filamin A, or in M2 cells transiently transfected with filamin A. It is noteworthy that stimulation of plasma membrane expression of BK(Ca) channels also occurs when M2 cells are transfected with filamin A constructs that lack the actin binding domain and that do not bind actin in vivo or in vitro. Filamin A is necessary for normal trafficking of BK(Ca) channels to the plasma membrane, but this effect does not require interactions with actin microfilaments, and it is possible that other actions of the filamin family of scaffolding proteins are independent of effects on actin.

journal_name

Mol Pharmacol

journal_title

Molecular pharmacology

authors

Kim EY,Ridgway LD,Dryer SE

doi

10.1124/mol.107.038026

subject

Has Abstract

pub_date

2007-09-01 00:00:00

pages

622-30

issue

3

eissn

0026-895X

issn

1521-0111

pii

mol.107.038026

journal_volume

72

pub_type

杂志文章

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