Abstract：
:The ATP signaling mechanism in neuroblastoma x glioma hybrid NG108-15 cells differentiated by exposure to dibutyryl-cAMP was characterized. In cells loaded with fura-2, ATP rapidly raised the cytosolic Ca2+ concentration ([Ca2+]i); the magnitude of the rise was inversely proportional to the extracellular Na+ concentration. Large increases in cytosolic Na+ concentration, measured with the fluorescent Na+ indicator sodium-binding benzofuran isophthalate, were dose-dependently elicited by ATP. ATP also evoked the entry of ethidium bromide into cells, and this process was inhibited by Mg2+. Inositol-1,4,5-trisphosphate (IP3) generation induced by ATP was totally blocked by removal of extracellular Ca2+, but residual IP3 generation still remained in nondifferentiated cells. In addition, ATP produced a concentration-, time-, and Mg(2+)-dependent biphasic uptake of 45Ca2+. A range of nucleotides and ATP analogues, including CTP, UTP, and GTP, induced only 9-29% of the ATP response. However, adenosine 5'-thiotriphosphate evoked 79% of ATP-induced 45Ca2+ uptake. 45Ca2+ uptake elicited by ATP could be potently blocked by purinoceptor antagonists, but other tested reagents less effectively blocked the action of ATP. When bradykinin was used as an agonist, the [Ca2+]i rise was transient and was insensitive to the extracellular Na+ concentration. Na+ influx, entry of ethidium bromide, and 45Ca2+ uptake were unaffected by bradykinin. Furthermore, bradykinin-evoked IP3 generation was insensitive to extracellular Ca2+. Neither ATP nor bradykinin had any effect on cAMP levels within cells. These data suggest that ATP induces a [Ca2+]i rise in differentiated NG108-15 cells via two distinct Ca2+ influx mechanisms, i.e., a receptor-operated cation channel and pores formed by ATP4-. These mechanisms are distinct from those elicited by bradykinin.
journal_name
Mol Pharmacoljournal_title
Molecular pharmacologyauthors
Chueh SH,Hsu LS,Song SLsubject
Has Abstractpub_date
1994-03-01 00:00:00pages
532-9issue
3eissn
0026-895Xissn
1521-0111journal_volume
45pub_type
杂志文章abstract：:P2X receptors are trimeric membrane proteins. When they bind extracellular ATP, a conformational change occurs that opens a transmembrane ion channel. The ATP-binding pocket is formed in a cleft between two subunits, and a critical amino acid residue for ATP contact is Lys⁶⁹ (P2X2 numbering). In the present work, we s...
journal_title：Molecular pharmacology
pub_type： 杂志文章
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journal_title：Molecular pharmacology
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doi：
更新日期：1992-07-01 00:00:00
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journal_title：Molecular pharmacology
pub_type： 杂志文章
doi：
更新日期：1993-06-01 00:00:00
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pub_type： 杂志文章
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journal_title：Molecular pharmacology
pub_type： 杂志文章
doi：
更新日期：1992-08-01 00:00:00
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pub_type： 杂志文章,评审
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journal_title：Molecular pharmacology
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
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journal_title：Molecular pharmacology
pub_type： 杂志文章
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更新日期：1998-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2007-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：2005-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：2001-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2002-12-01 00:00:00
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journal_title：Molecular pharmacology
pub_type： 杂志文章
doi：
更新日期：1993-05-01 00:00:00
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journal_title：Molecular pharmacology
pub_type： 杂志文章
doi：
更新日期：1984-05-01 00:00:00
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journal_title：Molecular pharmacology
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
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更新日期：2007-04-01 00:00:00
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journal_title：Molecular pharmacology
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
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journal_title：Molecular pharmacology
pub_type： 杂志文章
doi：
更新日期：1991-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：1999-02-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2014-06-01 00:00:00
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journal_title：Molecular pharmacology
pub_type： 杂志文章
doi：
更新日期：1982-03-01 00:00:00
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pub_type： 杂志文章
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更新日期：2004-03-01 00:00:00
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journal_title：Molecular pharmacology
pub_type： 杂志文章
doi：10.1124/mol.108.053629
更新日期：2009-04-01 00:00:00
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journal_title：Molecular pharmacology
pub_type： 杂志文章
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更新日期：2005-07-01 00:00:00
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journal_title：Molecular pharmacology
pub_type： 杂志文章
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更新日期：2008-11-01 00:00:00
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journal_title：Molecular pharmacology
pub_type： 杂志文章
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更新日期：2004-04-01 00:00:00
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pub_type： 杂志文章
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更新日期：1999-11-01 00:00:00
