Effect of calcitonin gene-related peptide on anastomotic healing in the presence of endotoxin.

Abstract:

BACKGROUND:Intestinal anastomotic healing is a complex procedure in which several mediators, cytokines and other substances play roles, as well as calcitonin gene-related peptide (CGRP). CGRP is capable of stimulating DNA synthesis and cell proliferation in endothelial cells by increasing vasodilatation and inflammatory response and promoting epithelial, vascular and mesothelial cell proliferation. This study was undertaken to investigate whether CGRP has a beneficial effect on intestinal anastomotic healing, even in septic conditions. METHODS:Four groups of 10 rats were administered normal saline (0.5 mL), lipopolysaccharide (LPS) (0.5 mg/kg), CGRP (0.5 mL 6.5 x 10(-10) mol/L) and LPS + CGRP (0.5 mg/kg + 0.5 mL 6.5 x 10(-10) mol/L) via intraperitoneal route, respectively, 24 h prior to operation and postoperatively. All rats underwent ileo-ileal end-to-end anastomosis. Anastomotic bursting pressure and tissue hydroxyproline levels were measured on postoperative day 7. RESULTS:Calcitonin gene-related peptide was found to have positive effects on both parameters of healing. The LPS-injected group showed intestinal anastomotic healing disorder suggesting impaired collagen production, which showed improvement after CGRP administration. CONCLUSIONS:Calcitonin gene-related peptide increases anastomotic wound healing in experimental intestinal anastomosis in the presence of endotoxin.

journal_name

ANZ J Surg

journal_title

ANZ journal of surgery

authors

Dogmuş M,Ersoy E,Anadol AZ,Ozel U,Bilgehan A

doi

10.1111/j.1445-2197.2005.03616.x

keywords:

subject

Has Abstract

pub_date

2005-12-01 00:00:00

pages

1106-10

issue

12

eissn

1445-1433

issn

1445-2197

pii

ANS3616

journal_volume

75

pub_type

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