Small interfering RNA-induced CHFR silencing sensitizes oral squamous cell cancer cells to microtubule inhibitors.


:Alterations in the function of cell cycle checkpoints are frequently detected in oral squamous cell carcinomas (OSCCs), and are often associated with the sensitivity of the cancer cells to chemotherapeutic drugs. Recently, a mitotic checkpoint gene, Chfr, was shown to be inactivated by promoter methylation and point mutations in various human tumors. Here we show that the absence of its product, CHFR, is associated with mitotic checkpoint dysfunction, and that cancer cells lacking CHFR are sensitive to microtubule inhibitors. Checkpoint impairment appears to be caused by a prophase defect in this case, as OSCC cells lacking CHFR showed phosphorylation of histone H3 on Ser10 and translocation of cyclin B1 to the nucleus. When CHFR-deficient OSCC cells were treated with a microtubule inhibitor (docetaxel or paclitaxel), significant numbers of apoptotic cells were observed. Moreover, disruption of CHFR using small interfering RNA (siRNA) impaired the mitotic checkpoint, thereby reducing the ability of OSCC cells to arrest at G2/M phase and making them more sensitive to microtubule inhibitors. Our results suggest that CHFR could be a useful molecular target for chemotherapy.


Cancer Biol Ther


Cancer biology & therapy


Ogi K,Toyota M,Mita H,Satoh A,Kashima L,Sasaki Y,Suzuki H,Akino K,Nishikawa N,Noguchi M,Shinomura Y,Imai K,Hiratsuka H,Tokino T





Has Abstract


2005-07-01 00:00:00














  • Antitumor effects of a tumor cell vaccine expressing a membrane-bound form of the IL-12 p35 subunit.

    abstract::We investigated whether expression of the IL-12 p35 subunit in membrane-bound form in tumor cells enhanced their immunogenicity. Since p35 is only secreted when associated with the IL-12 p40 subunit, we generated tumor cells expressing membrane-bound forms of p35 and p40 as chimeras with the transmembrane/cytoplasmic ...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Lim HY,Ju HY,Chung HY,Kim YS

    更新日期:2010-08-15 00:00:00

  • Preliminary investigation of the inhibitory effects of the tyroservaltide (YSV) tripeptide on human hepatocarcinoma BEL-7402.

    abstract::This study aimed to investigate the inhibitory effect of tyroservaltide (YSV) on the human hepatocarcinoma BEL-7402 transplanted into nude mice and to explore its possible anti-tumor mechanism. Nude mice bearing xenografts of the human BEL-7402 hepatoma were given daily i.p. injections of YSV or saline (as a control) ...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Jia J,Lu R,Qiu S,Li H,Che X,Zhao P,Jin M,Yang H,Lin G,Yao Z

    更新日期:2005-09-01 00:00:00

  • Emodin enhances cytotoxicity of chemotherapeutic drugs in prostate cancer cells: the mechanisms involve ROS-mediated suppression of multidrug resistance and hypoxia inducible factor-1.

    abstract::The intrinsic or acquired resistance to multiple drugs (MDR) of cancer cells remains one of the main obstacles for chemotherapy. Development of small molecule targeting to hypoxia inducible factor-1 (HIF-1) has been recently proposed as strategy for treatments of drug-resistant solid tumors. In the present study, emod...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Huang XZ,Wang J,Huang C,Chen YY,Shi GY,Hu QS,Yi J

    更新日期:2008-03-01 00:00:00

  • Human prostate cancer ZIP1/zinc/citrate genetic/metabolic relationship in the TRAMP prostate cancer animal model.

    abstract::Prostate cancer is the second leading cause of cancer deaths among men. The availability of animal models that represent the events and factors that exist in the natural history and biology of human prostate cancer is essential in dealing with prostate cancer. In recent decades and presently, emphasis has been directe...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Costello LC,Franklin RB,Zou J,Feng P,Bok R,Swanson MG,Kurhanewicz J

    更新日期:2011-12-15 00:00:00

  • Deletion of MBD2 inhibits proliferation of chronic myeloid leukaemia blast phase cells.

    abstract::Aberrant methylation of tumour suppressor genes is associated with the progression to a blast crisis in chronic myeloid leukaemia (CML). Methyl-CpG-binding domain protein 2 (MBD2) has been studied as a "reader" of DNA methylation in many cancers, but its role in CML is unclear. We constructed cell models of a homozygo...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Cheng L,Tang Y,Chen X,Zhao L,Liu S,Ma Y,Wang N,Zhou K,Zhou J,Zhou M

    更新日期:2018-08-03 00:00:00

  • Daunomycin, an antitumor DNA intercalator, influences histone-DNA interactions.

    abstract::Although daunomycin and adriamycin are considered effective antitumor drugs and have been used in the clinic for over 40 years, their mechanism of action is still a matter of debate. We investigated the influence of daunomycin on interaction between linker or core histones and DNA in live HeLa cells in vitro, using im...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Wójcik K,Zarębski M,Cossarizza A,Dobrucki JW

    更新日期:2013-09-01 00:00:00

  • The promising role of nivolumab in renal cell cancers.

    abstract::The therapeutic efficacy of checkpoint inhibitors across numerous tumor types has resulted in approval for neoplasms such as melanoma and lung cancer. Nivolumab is a fully humanized IgG4 antibody that inhibits immune checkpoint between programmed death 1 (PD-1) on T cells and PD-1 ligand 1 (PD-L1) and ligand 2 (PD-L2)...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Gupta K,Tiu DY,Tiu J,Aragon-Ching JB

    更新日期:2016-01-01 00:00:00

  • Lack of p38 MAP kinase activation in TRAIL-resistant cells is not related to the resistance to TRAIL-mediated cell death.

    abstract::Activation of MAP kinases is involved in various cellular processes, including immunoregulation, inflammation, cell growth, cell differentiation, and cell death. To investigate the role of p38 MAP kinase activation in the signaling pathway of TRAIL-mediated apoptosis, we compared TRAIL-mediated MAP kinase activation i...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Zhang L,Zhu H,Davis JJ,Jacob D,Wu S,Teraishi F,Gutierrez A,Wang Y,Fang B

    更新日期:2004-03-01 00:00:00

  • Signaling pathways in adenoid cystic cancers: implications for treatment.

    abstract::Adenoid cystic cancers (ACC) in the head and neck are rare yet present a clinical dilemma. Although 5-y survivals are excellent, they have a propensity for late recurrences. Most of these cancers are initially treated with surgery followed by radiation. When recurrences happen, treatment options are limited both by th...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Gupta AK,Wilke WW,Taylor EN,Bodeker KL,Hoffman HT,Milhem MM,Buatti JM,Robinson RA

    更新日期:2009-10-01 00:00:00

  • Genomic instability is associated with lack of telomerase activation in ovarian cancer.

    abstract:INTRODUCTION:Malignant cells are capable of an unlimited number of cell divisions, either through production of telomerase, or through the alternate lengthening of telomere (ALT) mechanism. Yeast cells with genomic instability have been shown to survive in the absence of telomerase by increased recombination events. We...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Landen CN,Klingelhutz A,Coffin JE,Sorosky JI,Sood AK

    更新日期:2004-12-01 00:00:00

  • Combined therapy with the RANKL inhibitor RANK-Fc and rhApo2L/TRAIL/dulanermin reduces bone lesions and skeletal tumor burden in a model of breast cancer skeletal metastasis.

    abstract::In bone metastases, tumor cells interact with the bone microenvironment to induce osteoclastogenesis, leading to bone destruction and the growth factor release.  RANK ligand (RANKL) is essential for osteoclast formation, function, and survival.  Tumor cell-mediated osteolysis is thought to occur ultimately via inducti...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Holland PM,Miller R,Jones J,Douangpanya H,Piasecki J,Roudier M,Dougall WC

    更新日期:2010-04-01 00:00:00

  • Sunitinib inhibits papillary thyroid carcinoma with RET/PTC rearrangement but not BRAF mutation.

    abstract::Sunitinib, a multi-targeted tyrosine kinase inhibitor, is frequently incorporated into the management of papillary thyroid carcinoma refractory to standard therapies. Although clinical trials are in progress, the mechanism of action in papillary thyroid carcinomas is not clear, especially regarding the effect on BRAF ...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Jeong WJ,Mo JH,Park MW,Choi IJ,An SY,Jeon EH,Ahn SH

    更新日期:2011-09-01 00:00:00

  • Chetomin, a Hsp90/HIF1α pathway inhibitor, effectively targets lung cancer stem cells and non-stem cells.

    abstract::Non-small cell lung cancer (NSCLC) remains recalcitrant to effective treatment due to tumor relapse and acquired resistance. Cancer stem cells (CSCs) are believed to be one mechanism for relapse and resistance and are consequently considered promising drug targets. We report that chetomin, an active component of Chaet...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Min S,Wang X,Du Q,Gong H,Yang Y,Wang T,Wu N,Liu X,Li W,Zhao C,Shen Y,Chen Y,Wang X

    更新日期:2020-08-02 00:00:00

  • Proteomic profiling: a novel method for differential diagnosis?

    abstract::Tumors with identical phenotype can have markedly different biologic behavior. The differentiation between hemangioblastoma, a benign vascular brain tumor, and renal cell carcinoma (RCC), a malignant tumor that can metastasize to the brain, is a well-known pathological quandary. We report a 59-year-old female with von...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Gläsker S,Lonser RR,Okamoto H,Li J,Jaffee H,Oldfield EH,Zhuang Z,Vortmeyer AO

    更新日期:2007-03-01 00:00:00

  • Regulation of Myc function by ARF: checkpoint for Myc-induced oncogenesis.

    abstract::The alterative reading frame (ARF) protein is unique in its capacity to interact with Mdm2 thus facilitating p53-dependent cell cycle arrest and apoptosis. ARF also acts in a p53-independent manner in which it binds to Myc and interferes with transcriptional activation by Myc thereby inhibiting Myc-induced cell prolif...

    journal_title:Cancer biology & therapy

    pub_type: 评论,杂志文章


    authors: Sarkar D,Fisher PB

    更新日期:2006-06-01 00:00:00

  • Type B lactic acidosis, an uncommon paraneoplastic syndrome.

    abstract::A 67-year-old male presented with anasarca and persistent non-pruritic rash of lower extremities. Physical examination was positive for subcutaneous edema with a non-blanching rash of abdomen and lower extremities. Labs showed leukocytosis, lymphocytosis, anemia and thrombocytopenia. He also had acute kidney injury an...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Wahab A,Kesari K,J Smith S,Liu Y,Barta SK

    更新日期:2018-02-01 00:00:00

  • IDO inhibits T-cell function through suppressing Vav1 expression and activation.

    abstract:PURPOSE:Indoleamine 2,3-dioxygenase (IDO), a tryptophan catabolic enzyme, plays an important role in immune escape through suppressing T-cell function. Since Vav1 signaling pathway regulates T cell homeostasis, this study was designed to test the hypothesis that IDO induces T-cell immunosuppression through inhibiting V...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Li R,Wei F,Yu J,Li H,Ren X,Hao X

    更新日期:2009-07-01 00:00:00

  • Increasing the therapeutic index of 5-fluorouracil and 6-thioguanine by targeting loss of MTAP in tumor cells.

    abstract::Methylthioadenosine phosphorylase (MTAP), a key enzyme in the catabolism of 5'-deoxy-5'-methylthioadenosine (MTA), catalyzes the formation of adenine and 5-methylthioribose-1-phosphate. MTAP is expressed in all cells throughout the body, but a significant percentage of human tumors have lost MTAP expression, thereby m...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Tang B,Testa JR,Kruger WD

    更新日期:2012-09-01 00:00:00

  • Multiple tumor-suppressor genes on chromosome 3p contribute to head and neck squamous cell carcinoma tumorigenesis.

    abstract::Head and neck squamous cell carcinoma (HNSCC) remains a significant cause of morbidity and mortality. There has been a great interest in finding specific genomic changes which contribute to HNSCC tumorigenesis, especially within the chromosome 3p area, where high frequency of LOH (loss of heterozygosity) has been repo...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Lee DJ,Schönleben F,Banuchi VE,Qiu W,Close LG,Assaad AM,Su GH

    更新日期:2010-10-01 00:00:00

  • E2F1 as a target: promoter-driven suicide and small molecule modulators.

    abstract::Decreased requirements for mitogens and diminished sensitivity to antiproliferative signals are among the hallmarks of human cancer. These attributes are due, at least partly, to mutations that directly or indirectly compromise the function of the retinoblastoma tumor suppressor protein (pRB), which is a negative regu...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章,评审


    authors: Kaelin WG Jr

    更新日期:2003-07-01 00:00:00

  • Dissecting the roles of E1A and E1B in adenoviral replication and RCAd-enhanced RDAd transduction efficacy on tumor cells.

    abstract::Oncolytic viruses have recently received widespread attention for their potential in innovative cancer therapy. Many telomerase promoter-regulated oncolytic adenoviral vectors retain E1A and E1B. However, the functions of E1A and E1B proteins in the oncolytic role of replication-competent adenovirus (RCAd) and RCAd en...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Wei F,Wang H,Chen X,Li C,Huang Q

    更新日期:2014-10-01 00:00:00

  • Serial endoscopy in azoxymethane treated mice using ultra-high resolution optical coherence tomography.

    abstract:PURPOSE:Optical coherence tomography (OCT) is a minimally invasive, depth-resolved imaging tool that can be implemented in a small diameter endoscope for imaging mouse models of colorectal cancer (CRC). In this study, we utilized ultrahigh resolution (UHR) OCT to serially image the lower colon of azoxymethane (AOM) tre...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Hariri LP,Qiu Z,Tumlinson AR,Besselsen DG,Gerner EW,Ignatenko NA,Povazay B,Hermann B,Sattmann H,McNally J,Unterhuber A,Drexler W,Barton JK

    更新日期:2007-11-01 00:00:00

  • Combined endostatin and TRAIL gene transfer suppresses human hepatocellular carcinoma growth and angiogenesis in nude mice.

    abstract::Endostatin can inhibit tumor growth by blocking angiogenesis, whereas tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) may function as a soluble cytokine to selectively kill cancer cells without toxicity to most normal cells. To establish the combined anti-tumor therapeutic effect of endostatin and solu...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Zhang Y,Qu ZH,Cui M,Guo C,Zhang XM,Ma CH,Sun WS

    更新日期:2009-03-01 00:00:00

  • A targeted IL-15 fusion protein with potent anti-tumor activity.

    abstract::IL-15 has been actively investigated for its potential in tumor immunotherapy. To enhance the anti-tumor activity of IL-15, the novel PFC-1 construct was designed, which comprises the following 3 parts: (1) IL-15Rα fused with IL-15 to enhance IL-15 activity, (2) an Fc fragment to increase protein half-life, and (3) an...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Chen S,Huang Q,Liu J,Xing J,Zhang N,Liu Y,Wang Z,Li Q

    更新日期:2015-01-01 00:00:00

  • 17beta-estradiol and tamoxifen stimulate rapid and transient ERK activationin MCF-7 cells via distinct signaling mechanisms.

    abstract::Traditionally, estrogen signaling was thought to be mediated strictly through genomic pathways. Recently, however, it has been demonstrated that estrogen stimulation of cells leads to rapid nongenomic effects including ERK activation. While the precise mechanism of this action is still under investigation, it is known...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Visram H,Greer PA

    更新日期:2006-12-01 00:00:00

  • Enhanced growth inhibition and apoptosis induction in NSCLC cell lines by combination of celecoxib and 4HPR at clinically relevant concentrations.

    abstract::Celecoxib exhibits cancer preventive and therapeutic effects in animal models and clinical trials. It presumably acts through selective inhibition of cyclooxygenase-2 (COX-2) and subsequent reduction of prostaglandin (PG) synthesis. However, the concentrations of celecoxib required for growth inhibition and apoptosis ...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Sun SY,Schroeder CP,Yue P,Lotan D,Hong WK,Lotan R

    更新日期:2005-04-01 00:00:00

  • Overexpression of Ecto-5'-nucleotidase (CD73) promotes T-47D human breast cancer cells invasion and adhesion to extracellular matrix.

    abstract::Ecto-5'-nucleotidase (CD73) was overexpressed in malignancies of epithelial origin and was involved in a variety of cellular processes such as cytoprotection and anti-inflammation. In the present study, human mammary T-47D cells were transfected with pcDNA-NT5E to establish a CD73 overexpressed cell model. Short small...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Zhou P,Zhi X,Zhou T,Chen S,Li X,Wang L,Yin L,Shao Z,Ou Z

    更新日期:2007-03-01 00:00:00

  • Hypoxia promotes chemotherapy resistance by down-regulating SKA1 gene expression in human osteosarcoma.

    abstract::Drug resistance has always been the main problem in osteosarcoma treatment, and hypoxia seems to be one of the many causes for drug resistance. Therefore, in this study, we investigated how hypoxia triggers chemotherapy resistance in osteosarcoma. We first screened hypoxia- and normoxia- cultured osteosarcoma cells in...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Ma Q,Zhang Y,Liu T,Jiang K,Wen Y,Fan Q,Qiu X

    更新日期:2017-03-04 00:00:00

  • Role of GPER/GPR30 in tumoral testicular germ cells proliferation.

    abstract::In a recent issue of Cancer Biology & Therapy, Vitale et al. introduced the paper of Franco et al. reporting the expression of GPER (a seven-transmembrane-spanning receptor also known as GPR30) on testicular germ cell tumors (TGCTs). The paper commented on the possible pathophysiological role of this G-protein-coupled...

    journal_title:Cancer biology & therapy

    pub_type: 评论,杂志文章


    authors: Chevalier N,Bouskine A,Fenichel P

    更新日期:2011-07-01 00:00:00

  • Altered energy metabolism in cancer: a unique opportunity for therapeutic intervention.

    abstract::The early observations by Dr Otto Warburg revealed that fundamentally metabolic differences exist between malignant tumor cells and adjacent normal cells. Many studies have further reported the relationship between altered cellular metabolism and therapeutic outcomes. These observations suggest that targeting the pecu...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章,评审


    authors: Zhang Y,Yang JM

    更新日期:2013-02-01 00:00:00