Abstract:
:Derived from the maize Mu1 transposon, RescueMu provides strategies for maize gene discovery and mutant phenotypic analysis. 9.92 Mb of gene-enriched sequences next to RescueMu insertion sites were co-assembled with expressed sequence tags and analyzed. Multiple plasmid recoveries identified probable germinal insertions and screening of RescueMu plasmid libraries identified plants containing probable germinal insertions. Although frequently recovered parental insertions and insertion hotspots reduce the efficiency of gene discovery per plasmid, RescueMu targets a large variety of genes and produces knockout mutants.
journal_name
Genome Bioljournal_title
Genome biologyauthors
Fernandes J,Dong Q,Schneider B,Morrow DJ,Nan GL,Brendel V,Walbot Vdoi
10.1186/gb-2004-5-10-r82keywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
R82issue
10eissn
1474-7596issn
1474-760Xpii
gb-2004-5-10-r82journal_volume
5pub_type
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