Genome-wide mutagenesis of Zea mays L. using RescueMu transposons.

Abstract:

:Derived from the maize Mu1 transposon, RescueMu provides strategies for maize gene discovery and mutant phenotypic analysis. 9.92 Mb of gene-enriched sequences next to RescueMu insertion sites were co-assembled with expressed sequence tags and analyzed. Multiple plasmid recoveries identified probable germinal insertions and screening of RescueMu plasmid libraries identified plants containing probable germinal insertions. Although frequently recovered parental insertions and insertion hotspots reduce the efficiency of gene discovery per plasmid, RescueMu targets a large variety of genes and produces knockout mutants.

journal_name

Genome Biol

journal_title

Genome biology

authors

Fernandes J,Dong Q,Schneider B,Morrow DJ,Nan GL,Brendel V,Walbot V

doi

10.1186/gb-2004-5-10-r82

keywords:

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

R82

issue

10

eissn

1474-7596

issn

1474-760X

pii

gb-2004-5-10-r82

journal_volume

5

pub_type

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