Paenibacillus lactis sp. nov., isolated from raw and heat-treated milk.

Abstract:

:Endospore-forming bacteria were recovered from individual packages from different processing lines in a dairy plant during a tenacious periodical contamination of their UHT-milk production. Two colony types were seen, one of which was identified as Bacillus sporothermodurans. Analysis of the 16S rRNA gene of the second colony type placed these isolates within the genus Paenibacillus, with Paenibacillus lautus as the closest known relative. Moreover, over 99 % similarity was observed to the 16S rDNA sequence of MB 2035, a strain isolated previously from raw milk during a survey at dairy farms for very heat-resistant spore-forming bacteria. Nine other potentially closely related strains among the dairy farm isolates were found using rep-PCR typing. The taxonomic positions of these 19 isolates were further investigated using 16S rRNA gene sequencing and DNA-DNA hybridizations of representative strains. All 19 isolates shared a high degree of phenotypic similarity and were easily distinguished from closely related members of the genus. Anteiso-C(15 : 0), C(16 : 0) and iso-C(15 : 0) were among the major fatty acids and the genomic DNA G+C content was 51.6-51.7 mol%. Therefore, based on their phenotypic, phylogenetic and genomic distinctiveness, these 19 strains, isolated from both raw and heat-treated milk, are placed in the genus Paenibacillus as Paenibacillus lactis sp. nov. The type strain is MB 1871(T) (=LMG 21940(T)=DSM 15596(T)).

authors

Scheldeman P,Goossens K,Rodriguez-Diaz M,Pil A,Goris J,Herman L,De Vos P,Logan NA,Heyndrickx M

doi

10.1099/ijs.0.02822-0

keywords:

subject

Has Abstract

pub_date

2004-05-01 00:00:00

pages

885-891

issue

Pt 3

eissn

1466-5026

issn

1466-5034

journal_volume

54

pub_type

杂志文章
  • Sphaerisporangium rufum sp. nov., an endophytic actinomycete from roots of Oryza sativa L.

    abstract::An endophytic actinomycete, strain R10-82(T), isolated from surface-sterilized roots of rice (Oryza sativa L.) was studied using a polyphasic approach. Strain R10-82(T) produced branching substrate mycelia and developed spherical spore vesicles on aerial hyphae containing non-motile spores. The major cellular fatty ac...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.057885-0

    authors: Mingma R,Duangmal K,Trakulnaleamsai S,Thamchaipenet A,Matsumoto A,Takahashi Y

    更新日期:2014-04-01 00:00:00

  • Saturnispora bothae sp. nov., isolated from rotting wood.

    abstract::Two strains representing a novel species of the genus Saturnispora were isolated from rotting wood samples collected in an Atlantic Rainforest site in Brazil. Analyses of the sequences of the D1/D2 domains of the rRNA gene showed that this novel species belongs to a subclade in the Saturnispora clade formed by Saturni...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.001269

    authors: Morais CG,Lara CA,Borelli BM,Cadete RM,Moreira JD,Lachance MA,Rosa CA

    更新日期:2016-10-01 00:00:00

  • Prevotella brunnea sp. nov., isolated from a wound of a patient.

    abstract::A strain of an obligately anaerobic, Gram-stain-negative rod-shaped bacterium is described by phenotypical, biochemical and genotypical characterization. Strain A2672T was isolated from a wound of a patient sampled during routine care at hospital. Phylogenetic analysis was based on full-length 16S rRNA gene sequence a...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003715

    authors: Buhl M,Dunlap C,Marschal M

    更新日期:2019-12-01 00:00:00

  • Ramlibacter alkalitolerans sp. nov., alkali-tolerant bacterium isolated from soil of ginseng.

    abstract::A novel bacterial strain, designated CJ661T, was isolated from soil of ginseng in Anseong, South Korea. Cells of strain CJ661T were white-coloured, Gram-staining-negative, non-motile, aerobic and rod-shaped. Strain CJ661T grew optimally at 30 °C and pH 7.0. The analysis of 16S rRNA gene sequence of strain CJ661T showe...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002342

    authors: Lee DH,Cha CJ

    更新日期:2017-11-01 00:00:00

  • Genome-based reclassification of Lactobacillus casei: emended classification and description of the species Lactobacillus zeae.

    abstract::Taxonomic relationships between Lactobacillus casei, Lactobacillus paracasei and Lactobacillus zeae have long been debated. Results of previous analyses have shown that overall genome relatedness indices (such as average nucleotide identity and core nucleotide identity) between the type strains L. casei ATCC 393T and ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003969

    authors: Huang CH,Chen CC,Liou JS,Lee AY,Blom J,Lin YC,Huang L,Watanabe K

    更新日期:2020-06-01 00:00:00

  • Saccharopolyspora antimicrobica sp. nov., an actinomycete from soil.

    abstract::Three Gram-positive, aerobic, non-motile, non-acid-alcohol-fast strains, designated I05-00051, I05-00074T and I03-00808, were isolated from different soil samples in Beijing and Sichuan, China. Phylogenetic analysis based on 16S rRNA gene sequences and DNA-DNA hybridization experiments revealed that these three isolat...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65532-0

    authors: Yuan LJ,Zhang YQ,Guan Y,Wei YZ,Li QP,Yu LY,Li WJ,Zhang YQ

    更新日期:2008-05-01 00:00:00

  • Alistipes inops sp. nov. and Coprobacter secundus sp. nov., isolated from human faeces.

    abstract::Culture-based study of the faecal microbiome in two adult female subjects revealed the presence of two obligately anaerobic, non-spore-forming, rod-shaped, non-motile, Gram-negative bacterial strains that represent novel species. The first strain, designated 627T, was a fastidious, slow-growing, indole-positive bacter...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000617

    authors: Shkoporov AN,Chaplin AV,Khokhlova EV,Shcherbakova VA,Motuzova OV,Bozhenko VK,Kafarskaia LI,Efimov BA

    更新日期:2015-12-01 00:00:00

  • Marinicauda salina sp. nov., isolated from a marine solar saltern.

    abstract::A novel Gram-stain-negative, aerobic, oxidase-positive, motile, dimorphic rod bacterium, with a polar flagellum or a polar prostheca, designated as strain WD6-1T, was isolated from a sediment sample collected from a marine solar saltern located in Weihai, PR China. Growth of strain WD6-1T was observed at 15-45 °C (opt...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003441

    authors: Zhai TJ,Liu BT,Zhu RQ,Chen GJ,Du ZJ

    更新日期:2019-08-01 00:00:00

  • Amycolatopsis australiensis sp. nov., an actinomycete isolated from arid soils.

    abstract::The taxonomic position of a group of mesophilic actinomycetes isolated from arid Australian soils was determined using a polyphasic approach. The organisms shared chemical and morphological markers typical of members of the genus Amycolatopsis. They had identical 16S rRNA gene sequences and formed a distinct phyletic ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.64260-0

    authors: Tan GYA,Robinson S,Lacey E,Goodfellow M

    更新日期:2006-10-01 00:00:00

  • Paraliobacillus zengyii sp. nov., a slightly halophilic and extremely halotolerant bacterium isolated from Tibetan antelope faeces.

    abstract::Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125T and X-1174), which were Gram-stain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes. Their optimal temperature, NaCl concentration and pH for growth were ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003333

    authors: Wang X,Yang J,Lu S,Lai XH,Jin D,Pu J,Niu L,Zhu W,Liang J,Huang Y,Wang B,Wu X,Liang H,Xu J

    更新日期:2019-05-01 00:00:00

  • Castellaniella caeni sp. nov., a denitrifying bacterium isolated from sludge of a leachate treatment plant.

    abstract::A Gram-negative, non-motile, facultatively anaerobic, denitrifying bacterial strain, designated Ho-11(T), was isolated from sludge of a leachate treatment plant and was characterized taxonomically by using a polyphasic approach. The G+C content of the genomic DNA was 63.5 mol%. Strain Ho-11(T) contained ubiquinone Q-8...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65314-0

    authors: Liu QM,Ten LN,Im WT,Lee ST

    更新日期:2008-09-01 00:00:00

  • Aquimarina aggregata sp. nov., isolated from seawater.

    abstract::A novel Gram-stain-negative, agarolytic, rod-shaped, non-flagellated, strictly aerobic strain with gliding motility, designated RZW4-3-2T, was isolated from coastal seawater of the Yellow Sea in China (119.625° E 35.517° N). The organism grew optimally at 28 °C, at pH 7.0 and in the presence of 2.0-3.0 % (w/v) NaCl. T...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.001209

    authors: Wang Y,Ming H,Guo W,Chen H,Zhou C

    更新日期:2016-09-01 00:00:00

  • Snuella lapsa gen. nov., sp. nov., isolated from tidal flat sediment.

    abstract::A yellow-coloured, rod-shaped, Gram-reaction-negative, aerobic bacterial strain, designated JC2132(T), was isolated from a tidal flat sediment sample from Ganghwa Island, Korea. The isolate required sea salts for growth. Cells produced non-diffusible carotenoid pigments, but flexirubin-type pigments were absent. Glidi...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.027458-0

    authors: Yi H,Chun J

    更新日期:2011-10-01 00:00:00

  • Bacillus paraflexus sp. nov., isolated from compost.

    abstract::A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium capable of growing at 15-42 °C (optimum 30 °C) and at pH 5-11 (optimum pH 7) was isolated from compost. Its taxonomic position was deduced using a polyphasic approach and the strain was designated RC2(T). 16S rRNA gene sequence analysis showed tha...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.048223-0

    authors: Chandna P,Mayilraj S,Kuhad RC

    更新日期:2013-12-01 00:00:00

  • Parvibacter caecicola gen. nov., sp. nov., a bacterium of the family Coriobacteriaceae isolated from the caecum of a mouse.

    abstract::A single strain, NR06(T), was isolated from the intestine of a TNF(deltaARE) mouse. Based on phylogenetic analysis of partial 16S rRNA gene sequences, strain NR06(T) belongs in the family Coriobacteriaceae within the Actinobacteria. The most closely related species with validly published names are members of the gener...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.045344-0

    authors: Clavel T,Charrier C,Wenning M,Haller D

    更新日期:2013-07-01 00:00:00

  • Characterization of Micrococcus antarcticus sp. nov., a psychrophilic bacterium from Antarctica.

    abstract::A Gram-positive, cold-adapted, aerobic, spherical actinobacterium (strain T2T) with a quite low cardinal growth temperature was isolated from Chinese Great-Wall station in Antarctica. Sequence comparisons of the 16S rDNA indicated the isolate to be a phylogenetic member of the genus Micrococcus, family Micrococcaceae,...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-50-2-715

    authors: Liu H,Xu Y,Ma Y,Zhou P

    更新日期:2000-03-01 00:00:00

  • Proposal to modify Rule 10a and to delete Recommendation 10a(3) from the International Code of Nomenclature of Prokaryotes.

    abstract::Principle 2 of the Prokaryotic Code, as modified by the ICSP in 1999, reads: 'The nomenclature of prokaryotes is not independent of botanical and zoological nomenclature. When naming new taxa in the rank of genus or higher, due consideration is to be given to avoiding names which are regulated by the International Cod...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002247

    authors: Oren A

    更新日期:2017-09-01 00:00:00

  • Dactylosporangium tropicum sp. nov., isolated from soil.

    abstract::Two novel actinomycete strains, designated KB2-4(T) and KB9-2, were isolated from soil samples. Both isolates formed finger-shaped sporangia on short sporangiophores that emerged directly from substrate hyphae. The cell-wall peptidoglycan contained glutamic acid, glycine, alanine and meso-diaminopimelic acid; the whol...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.028829-0

    authors: Thawai C,Tanasupawat S,Kudo T

    更新日期:2011-10-01 00:00:00

  • Bacteroides intestinalis sp. nov., isolated from human faeces.

    abstract::During studies of the microbiota of human faeces, five strains of Gram-negative anaerobic rods were isolated following growth in a polyamine-deficient medium. These strains belonged to the genus Bacteroides on the basis of 16S rRNA gene sequence data. 16S rRNA gene sequence similarity between one of the strains, 341T,...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63914-0

    authors: Bakir MA,Kitahara M,Sakamoto M,Matsumoto M,Benno Y

    更新日期:2006-01-01 00:00:00

  • Phytohabitans suffuscus gen. nov., sp. nov., an actinomycete of the family Micromonosporaceae isolated from plant roots.

    abstract::An actinomycete strain, K07-0523(T), was isolated from the roots of an orchid collected in Okinawa prefecture, Japan. 16S rRNA gene sequence analysis indicated that the new strain belonged to the family Micromonosporaceae and the similarity values between strain K07-0523(T) and the type species of 24 genera in the fam...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.016477-0

    authors: Inahashi Y,Matsumoto A,Danbara H,Ōmura S,Takahashi Y

    更新日期:2010-11-01 00:00:00

  • Marinobacter pelagius sp. nov., a moderately halophilic bacterium.

    abstract::A Gram-negative, aerobic, neutrophilic, moderately halophilic bacterial strain, HS225(T), was isolated from seawater samples around the Zhoushan Archipelago, Zhejiang Province, China. The isolate grew optimally in media with 5.0 % NaCl, at pH 7.0-8.0 and at 25-30 degrees C. The predominant fatty acids were C(16 : 0)om...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65390-0

    authors: Xu XW,Wu YH,Wang CS,Yang JY,Oren A,Wu M

    更新日期:2008-03-01 00:00:00

  • Zygotorulaspora chibaensis sp. nov. and Zygotorulaspora danielsina sp. nov., novel ascomycetous yeast species from tree bark and soil.

    abstract::Multiple isolates belonging to the ascomycetous genus Zygotorulaspora were obtained from forest soils and tree bark in Shiba Prefecture in Japan, and Lake Daniels, Lewis Pass, in New Zealand. Phylogenetic analyses employing combined sequences of the D1/D2 domain and ITS region support the recognition of two new specie...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002889

    authors: Carvalho C,Tomás A,Libkind D,Imanishi Y,Sampaio JP

    更新日期:2018-08-01 00:00:00

  • Methylomonas lenta sp. nov., a methanotroph isolated from manure and a denitrification tank.

    abstract::Two methanotrophic bacteria, strains R-45377(T) and R-45370, were respectively isolated from a slurry pit of a cow stable and from a denitrification tank of a wastewater treatment plant in Belgium. The strains showed 99.9 % 16S rRNA gene sequence similarity. Cells were Gram-negative, motile rods containing type I meth...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.057794-0

    authors: Hoefman S,Heylen K,De Vos P

    更新日期:2014-04-01 00:00:00

  • Deinococcus aerolatus sp. nov. and Deinococcus aerophilus sp. nov., isolated from air samples.

    abstract::Two strains of pink-coloured bacteria, 5516T-9(T) and 5516T-11(T), were isolated from an air sample collected in Korea. The taxonomic status of these novel strains was investigated by means of a polyphasic approach. The novel strains were Gram-positive, aerobic, non-spore-forming and coccus-shaped bacteria. The DNA G+...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.016030-0

    authors: Yoo SH,Weon HY,Kim SJ,Kim YS,Kim BY,Kwon SW

    更新日期:2010-05-01 00:00:00

  • 'Candidatus Phytoplasma luffae', a novel taxon associated with witches' broom disease of loofah, Luffa aegyptica Mill.

    abstract::The phytoplasma associated with witches' broom disease of loofah [Luffa aegyptica Mill., syn. Luffa cylindrica (L.) M.J. Roem.] in Taiwan was classified in group 16SrVIII, subgroup A (16SrVIII-A), based on results from actual and in silico RFLP analysis of 16S rRNA gene sequences. Nucleotide sequencing of PCR-amplifie...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.001980

    authors: Davis RE,Zhao Y,Wei W,Dally EL,Lee IM

    更新日期:2017-08-01 00:00:00

  • Kordiimonas pumila sp. nov., isolated from coastal sediment.

    abstract::A novel Gram-stain-negative, translucent-white, aerobic, motile and rod-shaped strain, designated N18T, was isolated from a coastal sediment sample collected in Zhoushan, Zhejiang Province, China. 16S rRNA gene similarity analysis revealed that strain N18T demonstrated highest similarity to the genus Kordiimonas(95.3-...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002740

    authors: Ju Z,Zhang R,Hou XJ,Han SB,Li Y,Sun C,Wu M,Xu L

    更新日期:2018-05-01 00:00:00

  • Fodinibius salinus gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt mine.

    abstract::A novel, moderately halophilic, rod-shaped bacterium, designated strain YIM D17(T), was isolated from a sample of sediment from a salt mine in Yunnan, south-western China. The taxonomy of strain YIM D17(T) was investigated using a polyphasic approach. Strain YIM D17(T) was Gram-stain-negative, strictly aerobic and non...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.025502-0

    authors: Wang YX,Liu JH,Xiao W,Zhang XX,Li YQ,Lai YH,Ji KY,Wen ML,Cui XL

    更新日期:2012-02-01 00:00:00

  • Nocardioides fonticola sp. nov., a novel actinomycete isolated from spring water.

    abstract::A Gram-positive, rod-shaped, yellow-pigmented actinomycete, designated strain NAA-13T, was isolated from a freshwater spring located in Kaoshiung, southern Taiwan. This strain was characterized using a polyphasic taxonomic approach. The organism had chemotaxonomic and morphological properties consistent with its class...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65810-0

    authors: Chou JH,Cho NT,Arun AB,Young CC,Chen WM

    更新日期:2008-08-01 00:00:00

  • Larkinella ripae sp. nov., isolated from seashore soil.

    abstract::Strain 15J11-11T was isolated from soil collected at the seashore and was Gram-staining-negative, short-rod-shaped, gliding and pale-pink pigmented. Flexirubin-type pigments were absent. The isolate grew at a temperature range of 15 to 30 °C and a pH range of 7 to 8. Comparative 16S rRNA gene sequence studies showed t...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002188

    authors: Lee YH,Lee JJ,Lee SY,Lee DS,Kim MK,Ten LN,Jung HY

    更新日期:2017-10-01 00:00:00

  • Deinococcus budaensis sp. nov., a mesophilic species isolated from a biofilm sample of a hydrothermal spring cave.

    abstract::Following the exposure of a biofilm sample from a hydrothermal spring cave (Gellért Hill, Budapest, Hungary) to gamma radiation, a strain designated FeSTC15-38T was isolated and studied by polyphasic taxonomic methods. The spherical-shaped cells stained Gram-negative, and were aerobic and non-motile. The pH range for ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.001519

    authors: Makk J,Tóth EM,Anda D,Pál S,Schumann P,Kovács AL,Mádl-Szőnyi J,Márialigeti K,Borsodi AK

    更新日期:2016-12-01 00:00:00