Effects of trapidil on the healing of colonic anastomoses in an experimental rat model.

Abstract:

BACKGROUND:Trapidil has various properties including vasodilatation, inhibition of lipid peroxidation and platelet aggregation as well as, and reduction of, the inflammatory response to injury. The aim of the present study was to investigate the effects of trapidil on dexamethasone-impaired colonic anastomotic healing in an experimental rat model. METHODS:Twenty-four Wistar rats underwent colonic transsection and primary anastomosis. Rats were divided into four groups of six: group 1 (G1), control; group 2 (G2) trapidil, 8 mg/kg per day intravenously; group 3 (G3) dexamethasone, 0.1 mg/kg per day intramuscularly; and group 4 (G4) dexamethasone 0.1 mg/kg intramuscularly and trapidil 8 mg/kg intravenously per day, for 1 week. Anastomotic bursting pressure, hydroxyproline level, histopathological grading, malondialdehyde and nitrite/nitrate levels were determined. RESULTS:Dexamethasone-impaired anastomotic healing was found to be improved by trapidil administration in terms of anastomotic bursting pressure and hydroxyproline content (P = 0.026, and P = 0.017). In addition, histopathological examination revealed an increase in fibroblast proliferation and collagen deposition (P = 0.004, and P = 0.015) and a decrease in leucocyte infiltration (P = 0.004). Moreover, serum nitrite/nitrate and malondialdehyde levels decreased when G3 was compared to G4 (P < 0.001, P = 0.38). CONCLUSIONS:Trapidil may improve the dexamethasone-impaired anastomotic healing due to its preventive effects on inflammatory response and lipid peroxidation in rats.

journal_name

ANZ J Surg

journal_title

ANZ journal of surgery

authors

Colak T,Nayci A,Polat G,Polat A,Comelekoglu U,Kanik A,Turkmenoglu O,Aydin S

doi

10.1046/j.1445-2197.2003.02683.x

keywords:

subject

Has Abstract

pub_date

2003-11-01 00:00:00

pages

916-21

issue

11

eissn

1445-1433

issn

1445-2197

pii

2683

journal_volume

73

pub_type

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