当前位置： SCI文献检索 > EUROPEAN UROLOGY期刊下所有文献 > Methylation-specific PCR for DNA-based detection of occult tumor cells in lymph nodes of prostate cancer patients.

Methylation-specific PCR for DNA-based detection of occult tumor cells in lymph nodes of prostate cancer patients.

Abstract：

OBJECTIVES:To assess the feasibility of methylation-specific PCR (MSP) for the detection of promoter hypermethylation of the detoxifying glutathione-S-transferase P1 gene (GSTP1) to detect occult prostate cancer cells in lymph nodes (LNs). METHODS:Paraffin-embedded pelvic LNs from 20 patients with pT2pN0M0R0 prostate cancer who developed PSA relapse were assessed by MSP. In 18 of the patients, samples of the primary tumor were obtained for MSP. In 19 patients, bone marrow (BM) aspirates were analyzed preoperatively for disseminated tumor cells by immunocytochemistry (mAb A45-B/B3). In 16 patients, biopsies of the anastomotic region were performed following PSA relapse. As a negative control GSTP1 methylation status was also assessed in LNs from 9 patients for whom an autopsy was performed for non-cancer-related causes. RESULTS:All primary tumors displayed GSTP1 hypermethylation (HM). Preoperative BM assessment showed disseminated tumor cells in 8/20 cases (40%). In 4 patients, biopsies of the vesico-urethral anastomosis showed local tumor recurrence. The LNs in the cancer patients showed GSTP1 HM in 18/20 cases (90%) versus 1/9 patients (11.1%) in the non-cancer cohort (p<0.0001). CONCLUSION:GSTP1 HM appears to be well suited for molecular staging of prostate cancer and accurately detects disseminated tumor cells in LNs, which was seen in 90% of the patients with PSA relapse. However, the limited number of patients and the finding of benign and malignant prostatic tissue at the vesico-urethral anastomosis as a putative local source of PSA recurrence does not allow us to draw conclusions on the prognostic significance of our findings, yet.

journal_name

Eur Urol

journal_title

European urology

authors

Köllermann J,Müller M,Goessl C,Krause H,Helpap B,Pantel K,Miller K

doi

10.1016/s0302-2838(03)00361-0

keywords:

subject

Has Abstract

pub_date

2003-11-01 00:00:00

pages

533-8

issue

5

eissn

0302-2838

issn

1873-7560

pii

S0302283803003610

journal_volume

44

pub_type

杂志文章

相关文献

EUROPEAN UROLOGY文献大全
  • The role of the complexed-to-total prostate-specific antigen ratio in predicting the final pathological stage of clinically localized prostate cancer.

    abstract：OBJECTIVES:To examine the association between the complexed-to-total (C:T) prostate-specific antigen (PSA) ratio and prostate cancer pathological stage to assess whether the C:T PSA ratio may predict the final pathological stage in patients with clinically localized prostate cancer. PATIENTS AND METHODS:In a prospecti...

    journal_title：European urology

    pub_type： 临床试验,杂志文章

    doi：10.1016/s0302-2838(03)00149-0

    authors： Martínez M,Navarro S,Medina P,Villavicencio H,Rioja L,Solsona E,Estellés A,Aznar J,Jiménez-Cruz JF,España F

    更新日期：2003-06-01 00:00:00

  • In vitro chemosensitivity testing of KK-47 bladder cancer cell line: a short-term culture technique.

    abstract：:We present our modified technique of short-term culture assay. As an experimental set-up we used the KK-47 bladder cancer cell line for chemosensitivity testing. The technique is very easy and requires only 3 days. The results were found to be reproducible. ...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1159/000472528

    authors： Bazeed MA,Schärfe T,Becht E,Schmidt J,Jacobi GH,Thüroff JW

    更新日期：1985-01-01 00:00:00

  • Updated Guidelines for Metastatic Hormone-sensitive Prostate Cancer: Abiraterone Acetate Combined with Castration Is Another Standard.

    abstract：:Addition of docetaxel to androgen deprivation therapy (ADT) was recommended for patients with newly diagnosed metastatic hormone-sensitive prostate cancer (mHSPC), who are fit enough to receive docetaxel. Two recently published trials showed that the addition of abiraterone acetate plus prednisone to ADT has a clear s...

    journal_title：European urology

    pub_type： 社论

    doi：10.1016/j.eururo.2017.09.029

    authors： Mottet N,De Santis M,Briers E,Bourke L,Gillessen S,Grummet JP,Lam TB,van der Poel HG,Rouvière O,van den Bergh RCN,Cornford P

    更新日期：2018-03-01 00:00:00

  • Cardiovascular Mortality Following Short-term Androgen Deprivation in Clinically Localized Prostate Cancer: An Analysis of RTOG 94-08.

    abstract：BACKGROUND:Androgen deprivation therapy (ADT) is associated with coronary heart disease and diabetes in men with prostate cancer (PCa); however, controversy exists regarding ADT and cardiovascular mortality (CVM) with limited data for lower risk disease. OBJECTIVE:We conducted a hypothesis-generating retrospective ana...

    journal_title：European urology

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.eururo.2015.08.027

    authors： Voog JC,Paulus R,Shipley WU,Smith MR,McGowan DG,Jones CU,Bahary JP,Zeitzer KL,Souhami L,Leibenhaut MH,Rotman M,Husain SM,Gore E,Raben A,Chafe S,Sandler HM,Efstathiou JA

    更新日期：2016-02-01 00:00:00

  • Does the extent of lymphadenectomy in radical cystectomy for bladder cancer influence disease-free survival? A prospective single-center study.

    abstract：BACKGROUND:Controversy exists regarding the optimal extent of lymphadenectomy and the number of lymph nodes to be retrieved at radical cystectomy (RC). OBJECTIVE:To compare the disease-free survival of patients with standard lymphadenectomy (endopelvic region composed of the internal, external iliac, and obturator gro...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1016/j.eururo.2011.05.062

    authors： Abol-Enein H,Tilki D,Mosbah A,El-Baz M,Shokeir A,Nabeeh A,Ghoneim MA

    更新日期：2011-09-01 00:00:00

  • Urethral-sparing Robot-assisted Simple Prostatectomy: An Innovative Technique to Preserve Ejaculatory Function Overcoming the Limitation of the Standard Millin Approach.

    abstract：BACKGROUND:Despite recent technical innovations in the treatment of benign prostatic hyperplasia (BPH), retrograde ejaculation is still one of the most frequent adverse effects, with a major impact on patients' quality of life. OBJECTIVE:To evaluate the efficacy of our technique of urethral-sparing robotic-assisted si...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1016/j.eururo.2020.09.028

    authors： Porpiglia F,Checcucci E,Amparore D,Niculescu G,Volpi G,Piramide F,De Cillis S,Manfredi M,Autorino R,Fiori C

    更新日期：2020-10-05 00:00:00

  • Multiorgan failure 17 years after initial stone therapy: forgotten ureteral stent in a horseshoe kidney.

    abstract：:We report on the case of a 44-yr-old white man who was referred as an emergency from a local hospital to the medical intensive care unit (ICU) at our institution for progressive multiorgan failure of unknown cause. The CT scan of the abdomen showed a horseshoe kidney with extensive stone formation in both kidneys and ...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1016/j.eururo.2007.01.040

    authors： Pühse G,Piechota H,Scheffold C,Kloska S,Hertle L,Wülfing C

    更新日期：2007-12-01 00:00:00

  • Laparoscopic promontory sacral colpopexy: is the posterior, recto-vaginal, mesh mandatory?

    abstract：OBJECTIVE(S):The aim of our retrospective study was to determine if systematic placement of a posterior mesh, in addition to an anterior vesico-vaginal mesh, is necessary for laparoscopic treatment of pelvic organ prolapse. METHODS:A laparoscopic promontory sacral colpopexy was performed in 108 patients, including 55 ...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1016/j.eururo.2004.01.004

    authors： Antiphon P,Elard S,Benyoussef A,Fofana M,Yiou R,Gettman M,Hoznek A,Vordos D,Chopin DK,Abbou CC

    更新日期：2004-05-01 00:00:00

  • Laparoscopic partial nephrectomy for tumour excision in a horseshoe kidney.

    abstract：:A 62-yr-old woman with a 2-cm enhancing renal mass in the right moiety of a horseshoe kidney was treated via a four-port transperitoneal laparoscopic approach. The tumour was excised by cold scissors, and the parenchyma was sutured with Vicryl 1 sutures with Surgicel bolster and by using pledgets of Hem-o-lok((R)) cli...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1016/j.eururo.2006.10.052

    authors： Tsivian A,Shtricker A,Benjamin S,Sidi AA

    更新日期：2007-04-01 00:00:00

  • Toxic catheters and diminished urethral blood circulation in the induction of urethral strictures.

    abstract：:Local effects of indwelling urinary catheters are poorly characterized. Latex catheter brands of various degrees of tissue toxicity were implanted into the urethra of 27 male piglets. The systemic hemodynamic states varied from normal to hypovolemia, where the circulation changes simulated the extracorporeal perfusion...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1159/000472651

    authors： Talja M,Virtanen J,Andersson LC

    更新日期：1986-01-01 00:00:00

  • Positive surgical margins and accessory pudendal artery preservation during laparoscopic radical prostatectomy.

    abstract：PURPOSE:To determine whether preservation of accessory pudendal arteries (APAs) adversely influences the rate of positive surgical margins (PSMs) during laparoscopic radical prostatectomy (LRP). MATERIAL AND METHODS:Between January 2003 and January 2005, 377 men with clinically localized prostate cancer underwent a LR...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1016/j.eururo.2005.08.006

    authors： Secin FP,Karanikolas N,Kuroiwa K,Vickers A,Touijer K,Guillonneau B

    更新日期：2005-11-01 00:00:00

  • Neuron-specific enolase: a serum tumor marker in renal cell carcinoma?

    abstract：:In previous reports, neuron-specific enolase (NSE) has been considered to be a useful tumor marker in renal cell carcinoma (RCC). This led us to perform a prospective clinical trial in this field. NSE was checked as a tumor marker for diagnosis and monitoring of patients with RCC. We report on 55 patients and their NS...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1159/000474336

    authors： Gross AJ,Michl UH,Bornhöft G,Dieckmann KP

    更新日期：1993-01-01 00:00:00

  • Current microbiological and clinical aspects of urinary tract infections.

    abstract：OBJECTIVES:To evaluate some risk factors which could affect the isolation rates of various uropathogens and their in vitro susceptibility to antibiotics in ambulatory and hospitalized patients. PATIENTS AND METHODS:A prospective study was conducted in a microbiological laboratory at Pisa Hospital. Nine-hundred and sev...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1159/000049813

    authors： Bonadio M,Meini M,Spitaleri P,Gigli C

    更新日期：2001-10-01 00:00:00

  • Activating AKT1 and PIK3CA Mutations in Metastatic Castration-Resistant Prostate Cancer.

    abstract：BACKGROUND:Activating mutations in AKT1 and PIK3CA are undercharacterised in metastatic castration-resistant prostate cancer (mCRPC), but are linked to activation of phosphatidylinositol 3-kinase (PI3K) signalling and sensitivity to pathway inhibitors in other cancers. OBJECTIVE:To determine the prevalence, genomic co...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1016/j.eururo.2020.04.058

    authors： Herberts C,Murtha AJ,Fu S,Wang G,Schönlau E,Xue H,Lin D,Gleave A,Yip S,Angeles A,Hotte S,Tran B,North S,Taavitsainen S,Beja K,Vandekerkhove G,Ritch E,Warner E,Saad F,Iqbal N,Nykter M,Gleave ME,Wang Y,Annala

    更新日期：2020-12-01 00:00:00

  • Supra-ampullar cystectomy and ileal neobladder.

    abstract：OBJECTIVE:This article describes both the open and laparoscopic operative techniques of supra-ampullar cystectomy (SAC). METHODS:Both open (photographs and drawings) and laparoscopic (attached DVD) SAC are explained step by step. RESULTS:Between May 1984 and December 2005, 31 patients with bladder tumour underwent SA...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1016/j.eururo.2006.07.049

    authors： Terrone C,Porpiglia F,Cracco C,Tarabuzzi R,Cossu M,Renard J,Scarpa RM,Rocca Rossetti S

    更新日期：2006-12-01 00:00:00

  • Hypospadias: Duckett pedicle tube urethroplasty.

    abstract：:The Duckett transverse tube pedicle urethroplasty was used to repair the primary hypospadias of 190 boys over an 11-year period. The fistula rate was 34.4%. The majority of boys have obtained a satisfactory cosmetic and functional result with a single operation. ...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1159/000471657

    authors： Dewan PA,Dinneen MD,Winkle D,Duffy PG,Ransley PG

    更新日期：1991-01-01 00:00:00

  • Genetic Variants Related to Longer Telomere Length are Associated with Increased Risk of Renal Cell Carcinoma.

    abstract：BACKGROUND:Relative telomere length in peripheral blood leukocytes has been evaluated as a potential biomarker for renal cell carcinoma (RCC) risk in several studies, with conflicting findings. OBJECTIVE:We performed an analysis of genetic variants associated with leukocyte telomere length to assess the relationship b...

    journal_title：European urology

    pub_type： 杂志文章,meta分析,多中心研究

    doi：10.1016/j.eururo.2017.07.015

    authors： Machiela MJ,Hofmann JN,Carreras-Torres R,Brown KM,Johansson M,Wang Z,Foll M,Li P,Rothman N,Savage SA,Gaborieau V,McKay JD,Ye Y,Henrion M,Bruinsma F,Jordan S,Severi G,Hveem K,Vatten LJ,Fletcher T,Koppova K,Larsso

    更新日期：2017-11-01 00:00:00

  • A new case of pyelic fusion.

    abstract：:The quite uncommon case of a girl with two kidneys drained by one single pelvis and one ureter is reported and a review of the literature is presented. ...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1159/000473343

    authors： Bruezière J,Frétin J

    更新日期：1980-01-01 00:00:00

  • Eraser laser enucleation of the prostate: technique and results.

    abstract：BACKGROUND:Eraser, a 1318-nm diode laser, has been used for 15 yr for resection of lung metastases. It was recently introduced in urology for small kidney tumors and for the treatment of benign prostatic obstruction. OBJECTIVE:To demonstrate on video our technique of Eraser laser enucleation of the prostate (ELEP) and...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1016/j.eururo.2012.08.049

    authors： Hruby S,Sieberer M,Schätz T,Jones N,Zimmermann R,Janetschek G,Lusuardi L

    更新日期：2013-02-01 00:00:00

  • Postchemotherapy laparoscopic retroperitoneal lymph node dissection for low-volume, stage II, nonseminomatous germ cell tumor: first 100 patients.

    abstract：BACKGROUND:Retroperitoneal lymph node dissection (RPLND) is indicated after chemotherapy in case of radiologic incomplete remission or teratomatous elements in orchiectomy specimens. Open RPLND is associated with considerable morbidity, but technical difficulty of postchemotherapy laparoscopic RPLND (L-RPLND) can be si...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1016/j.eururo.2012.09.036

    authors： Steiner H,Leonhartsberger N,Stoehr B,Peschel R,Pichler R

    更新日期：2013-06-01 00:00:00

  • Sexual functioning of adult patients born with meningomyelocele. A pilot study.

    abstract：OBJECTIVES:This pilot study was started to get an impression of the sexual activity and sexual difficulties of adult patients born with meningomyelocele in control at Utrecht University Hospital, and to find out whether these problems deserve more attention from the Spina Bifida Team. METHODS:Seventeen patients (9 men...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1159/000019673

    authors： Vroege JA,Zeijlemaker BY,Scheers MM

    更新日期：1998-01-01 00:00:00

  • Long-term outcomes among noncuratively treated men according to prostate cancer risk category in a nationwide, population-based study.

    abstract：BACKGROUND:Limited data exist on long-term outcomes among men with prostate cancer (PCa) from population-based cohorts incorporating information on clinical risk category. OBJECTIVE:To assess 15-yr mortality for men with PCa treated with noncurative intent according to clinical stage, Gleason score (GS), serum levels ...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1016/j.eururo.2012.08.001

    authors： Rider JR,Sandin F,Andrén O,Wiklund P,Hugosson J,Stattin P

    更新日期：2013-01-01 00:00:00

  • Clean intermittent self-catheterization in the chronical management of the neurogenic bladder.

    abstract：:30 patients with neurogenic bladder dysfunction were treated with intermittent self-catheterization. Since the application of this method, urine loss has disappeared almost completely and the urinary infection rate has decreased. In some cases there was radiological improvement of the upper urinary tract. In all the o...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1159/000473301

    authors： Wyndaele JJ,Oosterlinck W,De Sy W

    更新日期：1980-01-01 00:00:00

  • Interferon-alpha suppresses the antiapoptotic effect of NF-kB and sensitizes renal cell carcinoma cells in vitro to chemotherapeutic drugs.

    abstract：BACKGROUND:Immunochemotherapy (ICT) with interleukin-2 (IL-2) and interferon-alpha (IFNalpha) with a secondary effector (5-fluorouracil, 5 FU) is the only promising treatment for advanced renal cell carcinoma (RCC). With IFNalpha, besides the activation mechanisms of the immunosystem, a direct antitumor effect on tumor...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1159/000052489

    authors： Steiner T,Junker U,Henzgen B,Nuske K,Durum SK,Schubert J

    更新日期：2001-04-01 00:00:00

  • Urinary retention in patients with BPH treated with finasteride or placebo over 4 years. Characterization of patients and ultimate outcomes. The PLESS Study Group.

    abstract：OBJECTIVES:Knowledge regarding the incidence and prevalence of acute urinary retention and the ultimate outcome is very limited. The purpose of the present analysis was to document the natural history and outcomes of acute urinary retention (AUR) further specified as being either precipitated or spontaneous, and to eva...

    journal_title：European urology

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1159/000020189

    authors： Roehrborn CG,Bruskewitz R,Nickel GC,Glickman S,Cox C,Anderson R,Kandzari S,Herlihy R,Kornitzer G,Brown BT,Holtgrewe HL,Taylor A,Wang D,Waldstreicher J

    更新日期：2000-05-01 00:00:00

  • Nephrostomy tube or 'JJ' ureteric stent in ureteric obstruction: assessment of patient perspectives using quality-of-life survey and utility analysis.

    abstract：INTRODUCTION:Upper urinary tract obstruction is often relieved by either a percutaneous nephrostomy tube (PCN) or a ureteric stent. Both can cause considerable morbidity and reduce patient's health-related quality of life (QoL). We have compared the QoL in these 2 groups. PATIENTS AND METHODS:34 patients (21 stent and...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1159/000052529

    authors： Joshi HB,Adams S,Obadeyi OO,Rao PN

    更新日期：2001-06-01 00:00:00

  • Intrafascial nerve-sparing endoscopic extraperitoneal radical prostatectomy.

    abstract：OBJECTIVES:Based on our recently published anatomic studies, we present the most recent refinement of the endoscopic extraperitoneal radical prostatectomy (EERPE), the intrafascial nerve-sparing EERPE (nsEERPE). METHODS:As part of the intrafascial technique, the dissection plane is directly on the prostatic capsule, f...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1016/j.eururo.2007.11.047

    authors： Stolzenburg JU,Rabenalt R,Do M,Schwalenberg T,Winkler M,Dietel A,Liatsikos E

    更新日期：2008-05-01 00:00:00

  • Impotence due to corporeal veno-occlusive dysfunction: long-term follow-up of venous surgery.

    abstract：:Corporeal veno-occlusive dysfunction is an important cause of organic impotence and is characterized by increased flow rates to create and to maintain erection during artificial erection produced by intracavernous saline infusion. Sixty-seven patients with this erectile insufficiency underwent penile ligature-resectio...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1159/000474815

    authors： Wespes E,Delcour C,Preserowitz L,Herbaut AG,Struyven J,Schulman C

    更新日期：1992-01-01 00:00:00

  • Surgical Safety of Radical Cystectomy and Pelvic Lymph Node Dissection Following Neoadjuvant Pembrolizumab in Patients with Bladder Cancer: Prospective Assessment of Perioperative Outcomes from the PURE-01 Trial.

    abstract：:No data are available on the surgical safety of radical cystectomy (RC) and pelvic lymph node dissection (PLND) after the administration of checkpoint inhibitors. We aimed at reporting the first prospective rigorous assessment of perioperative outcomes after RC and extended PLND following neoadjuvant pembrolizumab in ...

    journal_title：European urology

    pub_type： 杂志文章

    doi：10.1016/j.eururo.2019.12.019

    authors： Briganti A,Gandaglia G,Scuderi S,Gallina A,Colombo R,Fossati N,Barletta F,Pellegrino A,Nocera L,Montorsi F,Necchi A

    更新日期：2020-05-01 00:00:00

  • Is the diet of patients with interstitial cystitis related to their disease?

    abstract：OBJECTIVE:The dietary habits of interstitial cystitis (IC) patients compared to the average food and fluid consumption of the general population were evaluated and any spontaneous preference or avoidance of specific foodstuffs and fluids of IC patients was investigated. METHODS:A verbal interview with 16 IC patients p...

    journal_title：European urology

    pub_type： 杂志文章

    doi：

    authors： Bade JJ,Peeters JM,Mensink HJ

    更新日期：1997-01-01 00:00:00