Abstract：
:The membrane-permeable, noncompetitive inositol 1,4,5-trisphosphate (IP3)-receptor inhibitor 2-aminoethoxydiphenyl borane (2-APB) has been widely used to probe for IP3-receptor involvement in calcium signaling pathways. However, a number of recent studies in different cell types revealed other sites of action of 2-APB. In this study, we examined the influence of 2-APB on capacitative calcium entry and intracellular Ca2+ concentrations in rat basophilic leukemia (RBL-2H3 m1) cells. 2-APB was found to inhibit capacitative calcium entry, but at concentrations greater than 50 microM, a new effect of 2-APB was observed. When capacitative calcium entry was blocked with Gd3+, 2-APB caused an increase in cytoplasmic Ca2+. This increase in intracellular Ca2+ was not caused by altered buffering of cytoplasmic Ca2+ and was not caused by or in any way affected by the depletion of intracellular Ca2+ stores. Associated with the increase in intracellular Ca2+, in the presence of 2 mM Ca2+, 2-APB activated single channels in the plasma membrane with a conductance of approximately 50 pS. These channels seem to be nonselective cation channels; monovalent cations are the major carriers of current, but finite permeability to Ca2+ leads to a significant intracellular Ca2+ signal. Experiments with excised patches indicate that 2-APB activates these channels from the outer aspect of the cell membrane. This effect of 2-APB further illustrates the complex actions of this compound and reveals the presence in RBL-2H3 m1 cells of a novel, ligand-gated calcium-permeable channel.
journal_name
Mol Pharmacoljournal_title
Molecular pharmacologyauthors
Braun FJ,Aziz O,Putney JW Jrdoi
10.1124/mol.63.6.1304keywords:
subject
Has Abstractpub_date
2003-06-01 00:00:00pages
1304-11issue
6eissn
0026-895Xissn
1521-0111pii
63/6/1304journal_volume
63pub_type
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更新日期：1991-12-01 00:00:00
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更新日期：1982-11-01 00:00:00
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doi：
更新日期：1993-12-01 00:00:00
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doi：
更新日期：1985-04-01 00:00:00
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更新日期：1993-09-01 00:00:00
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更新日期：1991-11-01 00:00:00
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更新日期：1988-09-01 00:00:00
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