Abstract：
:Previously, we reported the presence of dual promoters, referred to as distal (DP) and proximal, with a negative regulatory element between them in the mouse mu-opioid receptor (mor) gene. Here we have identified a positive regulatory element influencing mor DP transcription, which contains multiple consensus binding motifs for Sox factors (sex-determining Sry-like high mobility group box-containing genes). In gel supershift assays, the Sox family member Sox18 bound directly to the multiple Sox consensus binding motifs of the mor DP enhancer. Overexpression of Sox18 cDNA increased luciferase activity regulated by the mor DP, and did so in a Sox18 concentration-dependent manner. In contrast, overexpression of another Sox member, Sox5, triggered no such trans-activation of mor DP-driven luciferase activity or DNA-protein binding activity. These results suggest that Sox18 directly and specifically stimulates mor gene expression, by trans-activating the mor DP enhancer.
journal_name
Mol Pharmacoljournal_title
Molecular pharmacologyauthors
Im HJ,Smirnov D,Yuhi T,Raghavan S,Olsson JE,Muscat GE,Koopman P,Loh HHdoi
10.1124/mol.59.6.1486keywords:
subject
Has Abstractpub_date
2001-06-01 00:00:00pages
1486-96issue
6eissn
0026-895Xissn
1521-0111journal_volume
59pub_type
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更新日期：1996-09-01 00:00:00
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doi：
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更新日期：1998-06-01 00:00:00
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更新日期：1988-05-01 00:00:00
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