当前位置： SCI文献检索 > MOLECULAR PHARMACOLOGY期刊下所有文献 > Time course of 72-kilodalton heat shock protein induction and appearance of trifluoroacetyl adducts in livers of halothane-exposed rats.

Time course of 72-kilodalton heat shock protein induction and appearance of trifluoroacetyl adducts in livers of halothane-exposed rats.

Abstract：

:Previous studies have shown that exposure of phenobarbital-pretreated rats to halothane in 10% O2 causes centrilobular necrosis, induces expression of the 72-kDa heat shock protein (HSP72), and produces several trifluoroacetylated adducts. In the present study the time course of development of the centrilobular lesion, as measured by histochemistry, was compared with the time course of appearance of both trifluoroacetylated adducts and HSP72, as measured by Western blotting. One group of 20 rats was pretreated with phenobarbital for 5 days, whereas a second group of two rats was left as untreated controls. Ten phenobarbital-pretreated rats were exposed for 2 hr to 1% halothane in 10% O2 and 10 were exposed to 1% halothane in 20% O2. At either 2, 4, 6, or 24 hr after exposure, livers were excised and frozen without fixation. Thin sections stained with hematoxylin and eosin demonstrated that centrilobular lesions occurred at 6 hr and became extensive at 24 hr in rats pretreated with phenobarbital and exposed to 1% halothane in 10% O2. The time course of appearance of both trifluoroacetylated adducts and HSP72 was determined by Western blotting. Trifluoroacetylated adducts appeared in all rats exposed to halothane by 2 hr, lasted until 6 hr, and then diminished by 24 hr. In contrast, HSP72 was induced only in the rats pretreated with phenobarbital and exposed to 1% halothane in 10% O2. HSP72 appeared in both the nuclear and supernatant fractions at 6 hr after exposure and was intense 24 hr after exposure.

journal_name

Mol Pharmacol

journal_title

Molecular pharmacology

authors

Lin WQ,van Dyke RA,Marsh HM,Trudell JR

subject

Has Abstract

pub_date

1994-10-01 00:00:00

pages

639-43

issue

4

eissn

0026-895X

issn

1521-0111

journal_volume

46

pub_type

杂志文章

相关文献

MOLECULAR PHARMACOLOGY文献大全
  • Differences in affinity and efficacy of benzodiazepine receptor ligands at recombinant gamma-aminobutyric acidA receptor subtypes.

    abstract：:Two forms of the multisubunit gamma-aminobutyric acid (GABA)A receptor were expressed in Xenopus oocytes by injecting mRNA encoding the bovine GABAA receptor subunit cDNAs for alpha 1 beta 1 gamma 2L and alpha 3 beta 1 gamma 2L. The properties of these two combinations were examined by electrophysiological recording o...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：

    authors： Wafford KA,Whiting PJ,Kemp JA

    更新日期：1993-02-01 00:00:00

  • Bifunctional DNA alkylator 1,3-bis(2-chloroethyl)-1-nitrosourea activates the ATR-Chk1 pathway independently of the mismatch repair pathway.

    abstract：:The presence of DNA damage initiates signaling through the ataxia-telangiectasia mutated kinase (ATM) and the ATM- and the Rad3-related kinase (ATR), which phosphorylate, thus activating, the checkpoint kinases (Chk) 1 and 2, which leads to cell cycle arrest. The bifunctional DNA alkylator 1,3-bis(2-chloroethyl)-1-nit...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：10.1124/mol.108.053124

    authors： Cui B,Johnson SP,Bullock N,Ali-Osman F,Bigner DD,Friedman HS

    更新日期：2009-06-01 00:00:00

  • Regulation of pulmonary and hepatic cytochrome P4501A expression in the rat by hyperoxia: implications for hyperoxic lung injury.

    abstract：:Supplemental oxygen therapy is frequently used in the treatment of pulmonary insufficiency, as is encountered in premature infants, and in patients with acute respiratory distress syndrome. However, hyperoxia causes lung damage in experimental animals and may do so in humans. Cytochrome P4501A enzymes have been implic...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：10.1124/mol.61.3.507

    authors： Couroucli XI,Welty SE,Geske RS,Moorthy B

    更新日期：2002-03-01 00:00:00

  • A Truncated Six Transmembrane Splice Variant MOR-1G Enhances Expression of the Full-Length Seven Transmembrane μ-Opioid Receptor through Heterodimerization.

    abstract：:The μ-opioid receptor gene undergoes extensive alternative splicing to generate an array of splice variants. One group of splice variants excludes the first transmembrane (TM) domain and contains six TM domains. These 6TM variants are essential for the action of a novel class of analgesic drugs, including 3-iodobenzoy...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：10.1124/mol.120.119453

    authors： Zhang T,Xu J,Pan YX

    更新日期：2020-10-01 00:00:00

  • Antibodies Targeting Chemokine Receptors CXCR4 and ACKR3.

    abstract：:Dysregulation of the chemokine system is implicated in a number of autoimmune and inflammatory diseases, as well as cancer. Modulation of chemokine receptor function is a very promising approach for therapeutic intervention. Despite interest from academic groups and pharmaceutical companies, there are currently few ap...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章,评审

    doi：10.1124/mol.119.116954

    authors： Bobkov V,Arimont M,Zarca A,De Groof TWM,van der Woning B,de Haard H,Smit MJ

    更新日期：2019-12-01 00:00:00

  • 125I-Tyro-sauvagine: a novel high affinity radioligand for the pharmacological and biochemical study of human corticotropin-releasing factor 2 alpha receptors.

    abstract：:Corticotropin-releasing factor (CRF) receptors encoded by two distinct genes have recently been identified and termed CRF1 and CRF2. CRF and the non-mammalian-related peptide sauvagine bind to and activate CRF1 receptors with high affinity and equal potency. Although CRF is significantly weaker at the CRF2 receptor, s...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：

    authors： Grigoriadis DE,Liu XJ,Vaughn J,Palmer SF,True CD,Vale WW,Ling N,De Souza EB

    更新日期：1996-09-01 00:00:00

  • Desensitization and Tolerance of Mu Opioid Receptors on Pontine Kölliker-Fuse Neurons.

    abstract：:Acute desensitization of mu opioid receptors is thought to be an initial step in the development of tolerance to opioids. Given the resistance of the respiratory system to develop tolerance, desensitization of neurons in the Kölliker-Fuse (KF), a key area in the respiratory circuit, was examined. The activation of G p...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：10.1124/mol.117.109603

    authors： Levitt ES,Williams JT

    更新日期：2018-01-01 00:00:00

  • Insights into the gating mechanism of the ryanodine-modified human cardiac Ca2+-release channel (ryanodine receptor 2).

    abstract：:Ryanodine receptors (RyRs) are intracellular membrane channels playing key roles in many Ca(2+) signaling pathways and, as such, are emerging novel therapeutic and insecticidal targets. RyRs are so named because they bind the plant alkaloid ryanodine with high affinity and although it is established that ryanodine pro...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：10.1124/mol.114.093757

    authors： Mukherjee S,Thomas NL,Williams AJ

    更新日期：2014-09-01 00:00:00

  • Allosteric ligands for the corticotropin releasing factor type 1 receptor modulate conformational states involved in receptor activation.

    abstract：:Allosteric modulators of G-protein-coupled receptors can regulate conformational states involved in receptor activation ( Mol Pharmacol 58: 1412-1423, 2000 ). This hypothesis was investigated for the corticotropin-releasing factor type 1 (CRF(1)) receptor using a novel series of ligands with varying allosteric effect ...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：10.1124/mol.107.042978

    authors： Hoare SR,Fleck BA,Gross RS,Crowe PD,Williams JP,Grigoriadis DE

    更新日期：2008-05-01 00:00:00

  • Expression of tandem glutathione S-transferase recombinant genes in COS cells for analysis of efficiency of protein expression and associated drug resistance.

    abstract：:Expression vectors were designed and constructed to achieve optimum production of two different isozymes of rat glutathione S-transferase (GST) (EC 2.5.1.18) in COS cells, for studies of drug resistance. Promoter-enhancer elements from the simian virus 40 (SV40) early-region or the mouse alpha 2(I)-collagen gene, GST ...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：

    authors： Manoharan TH,Welch PJ,Gulick AM,Puchalski RB,Lathrop AL,Fahl WE

    更新日期：1991-04-01 00:00:00

  • Involvement of phenyl radicals in iodonium inhibition of flavoenzymes.

    abstract：:Iodonium inhibition of the flavoenzymes neutrophil NADPH oxidase and cytochrome P450 reductase has been suggested to require reductive metabolism of the inhibitor to a phenyl radical. Inhibition would ultimately result from covalent attachment of phenyl radicals to either the flavin cofactor or adjacent amino acid sid...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：

    authors： O'Donnell VB,Smith GC,Jones OT

    更新日期：1994-10-01 00:00:00

  • The effect of thiopurine methyltransferase expression on sensitivity to thiopurine drugs.

    abstract：:Although the thiopurine drugs 6-mercaptopurine (6-MP) and 6-thioguanine (6-TG) are well established agents for the treatment of leukemia, controversies remain regarding their main mode of action. Previous evidence has suggested that although 6-TG exerts a cytotoxic effect through incorporation of 6-thioguanine nucleot...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：10.1124/mol.62.1.102

    authors： Coulthard SA,Hogarth LA,Little M,Matheson EC,Redfern CP,Minto L,Hall AG

    更新日期：2002-07-01 00:00:00

  • Polymorphism and ligand dependent changes in human glucagon-like peptide-1 receptor (GLP-1R) function: allosteric rescue of loss of function mutation.

    abstract：:The glucagon-like peptide-1 receptor (GLP-1R) is a key physiological regulator of insulin secretion and a major therapeutic target for the treatment of type II diabetes. However, regulation of GLP-1R function is complex with multiple endogenous peptides that interact with the receptor, including full-length (1-37) and...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：10.1124/mol.111.072884

    authors： Koole C,Wootten D,Simms J,Valant C,Miller LJ,Christopoulos A,Sexton PM

    更新日期：2011-09-01 00:00:00

  • 3'-Phosphoadenosine-5'-phosphosulfate: photoaffinity ligand for sulfotransferase enzymes.

    abstract：:Sulfation is an important pathway in the biotransformation of many drugs, xenobiotic compounds, neurotransmitters, and hormones. The sulfate donor for these reactions is 3'-phosphoadenosine-5'-phosphosulfate (PAPS). We set out to determine whether PAPS might serve as a photoaffinity ligand for sulfotransferase enzymes...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：

    authors： Otterness DM,Powers SP,Miller LJ,Weinshilboum RM

    更新日期：1991-01-01 00:00:00

  • Defining a cellular map of cAMP nanodomains.

    abstract：:By limiting unrestricted activation of intracellular effectors, compartmentalised signalling of cyclic nucleotides confers specificity to extracellular stimuli and is critical for the development and health of cells and organisms. Dissecting the molecular mechanisms that allow local control of cyclic nucleotide signal...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：10.1124/mol.119.118869

    authors： Schleicher K,Zaccolo M

    更新日期：2020-02-28 00:00:00

  • Structural domains of chimeric dopamine-noradrenaline human transporters involved in the Na(+)- and Cl(-)-dependence of dopamine transport.

    abstract：:Catecholamine transporters constitute the biological targets for several important drugs, including antidepressants, cocaine, and related compounds. Some information exists about discrete domains of these transporters that are involved in substrate translocation and uptake blockade, but delineation of domains mediatin...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：10.1124/mol.58.6.1404

    authors： Syringas M,Janin F,Mezghanni S,Giros B,Costentin J,Bonnet JJ

    更新日期：2000-12-01 00:00:00

  • Immortalized hypothalamic GT1-7 neurons express functional gamma-aminobutyric acid type A receptors.

    abstract：:Neuronal cell lines provide a source of pure populations of neurons and allow the properties of many neurotransmitter receptors to be studied. However, none of these cells have been reported to express functional gamma-aminobutyric acid (GABA)A receptors. Indeed, there have been no reports of cell lines expressing fun...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：

    authors： Hales TG,Kim H,Longoni B,Olsen RW,Tobin AJ

    更新日期：1992-08-01 00:00:00

  • Structural Insights into the Atomistic Mechanisms of Action of Small Molecule Inhibitors Targeting the KCa3.1 Channel Pore.

    abstract：:The intermediate-conductance Ca2+-activated K+ channel (KCa3.1) constitutes an attractive pharmacological target for immunosuppression, fibroproliferative disorders, atherosclerosis, and stroke. However, there currently is no available crystal structure of this medically relevant channel that could be used for structu...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：10.1124/mol.116.108068

    authors： Nguyen HM,Singh V,Pressly B,Jenkins DP,Wulff H,Yarov-Yarovoy V

    更新日期：2017-04-01 00:00:00

  • A novel pentamethoxyflavone down-regulates tumor cell survival and proliferative and angiogenic gene products through inhibition of IκB kinase activation and sensitizes tumor cells to apoptosis by cytokines and chemotherapeutic agents.

    abstract：:Most anticancer drugs have their origin in traditional medicinal plants. We describe here a flavone, 5,3'-dihydroxy-3,6,7,8,4'-pentamethoxyflavone (PMF), from the leaves of the Thai plant Gardenia obtusifolia, that has anti-inflammatory and anticancer potential. Because the nuclear factor-κB (NF-κB) pathway is linked ...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章,收录出版

    doi：10.1124/mol.110.067512

    authors： Phromnoi K,Reuter S,Sung B,Prasad S,Kannappan R,Yadav VR,Chanmahasathien W,Limtrakul P,Aggarwal BB

    更新日期：2011-02-01 00:00:00

  • The antiallergic mast cell stabilizers lodoxamide and bufrolin as the first high and equipotent agonists of human and rat GPR35.

    abstract：:Lack of high potency agonists has restricted analysis of the G protein-coupled receptor GPR35. Moreover, marked variation in potency and/or affinity of current ligands between human and rodent orthologs of GPR35 has limited their productive use in rodent models of physiology. Based on the reported modest potency of th...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：10.1124/mol.113.089482

    authors： MacKenzie AE,Caltabiano G,Kent TC,Jenkins L,McCallum JE,Hudson BD,Nicklin SA,Fawcett L,Markwick R,Charlton SJ,Milligan G

    更新日期：2014-01-01 00:00:00

  • The role of the seventh transmembrane region in high affinity binding of a beta 2-selective agonist TA-2005.

    abstract：:To determine the structural basis for binding subtype selective agonists in the beta-adrenergic receptor (beta AR), we examined the interaction of the mutant beta 2AR and chimeric beta 1/beta 2AR with a selective beta 2AR agonist, TA-2005 (8-hydroxy-5-[(1R)-1-hydroxy-2-[N-[(1R)-2-(p-methoxyphenyl)-1-methyle thy l] am...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：10.1124/mol.53.1.128

    authors： Kikkawa H,Isogaya M,Nagao T,Kurose H

    更新日期：1998-01-01 00:00:00

  • Effect of breast cancer resistance protein (Bcrp/Abcg2) on the disposition of phytoestrogens.

    abstract：:The effect of breast cancer resistance protein (Bcrp/Abcg2) on the disposition of the phytoestrogens daidzein, genistein, and coumestrol was investigated using Bcrp(-/-) mice. Expression of the genes for either mouse Bcrp or human BCRP in MDCK II cells induced apically directed transport of the three phytoestrogens, w...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：10.1124/mol.107.034751

    authors： Enokizono J,Kusuhara H,Sugiyama Y

    更新日期：2007-10-01 00:00:00

  • Pharmacological and histochemical distinctions between molecularly defined sarcolemmal KATP channels and native cardiac mitochondrial KATP channels.

    abstract：:A variety of direct and indirect techniques have revealed the existence of ATP-sensitive potassium (KATP) channels in the inner membranes of mitochondria. The molecular identity of these mitochondrial KATP (mitoKATP) channels remains unclear. We used a pharmacological approach to distinguish mitoKATP channels from cla...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：

    authors： Hu H,Sato T,Seharaseyon J,Liu Y,Johns DC,O'Rourke B,Marbán E

    更新日期：1999-06-01 00:00:00

  • Muscarinic responses and binding in a murine neuroblastoma clone (N1E-115). Selective loss with subculturing of the low-affinity agonist site mediating cyclic GMP formation.

    abstract：:Cells of the murine neuroblastoma clone N1E-115 possess muscarinic receptors that influence the intracellular level of cyclic nucleotides. The stimulation of [3H]cyclic GMP levels occurs only with intact cells and has an EC50 near the "low-affinity" agonist equilibrium dissociation constant (KL) determined by radiolig...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：

    authors： McKinney M,Stenstrom S,Richelson E

    更新日期：1984-09-01 00:00:00

  • Interactions between the Mas-related receptors MrgD and MrgE alter signalling and trafficking of MrgD.

    abstract：:When expressed via an inducible promoter in human embryonic kidney 293 cells, the rat Mas-related gene D (rMrgD) receptor responded to beta-alanine but not L-alanine by elevating intracellular [Ca(2+)], stimulating phosphorylation of the mitogenactivated protein kinases known as extracellular signal-regulated kinase (...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：10.1124/mol.105.018788

    authors： Milasta S,Pediani J,Appelbe S,Trim S,Wyatt M,Cox P,Fidock M,Milligan G

    更新日期：2006-02-01 00:00:00

  • Mastoparan increases the intracellular free calcium concentration in two insulin-secreting cell lines by inhibition of ATP-sensitive potassium channels.

    abstract：:The mechanisms underlying mastoparan-induced elevation of the intracellular free calcium concentration ([Ca2+]i) were investigated in the insulin-secreting cell lines RINm5F and HIT. In both cell types, micromolar concentrations of mastoparan induced a prompt increase of [Ca2+]i, measured as an increase in fura-2 fluo...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：

    authors： Eddlestone GT,Komatsu M,Shen L,Sharp GW

    更新日期：1995-04-01 00:00:00

  • The aryl hydrocarbon receptor interacts with transcription factor IIB.

    abstract：:The aryl hydrocarbon receptor (AHR) and its DNA binding partner, the AHR nuclear translocator (ARNT), are basic helix-loop-helix transcription factors that mediate many of the toxic and carcinogenic effects of polyhalogenated aromatic hydrocarbons. The basic regions of the AHR and ARNT contact the GCGTG recognition si...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：

    authors： Swanson HI,Yang JH

    更新日期：1998-10-01 00:00:00

  • Structural characteristics that govern binding to, and modulation through, the cardiac ryanodine receptor nucleotide binding site.

    abstract：:Comparative molecular field analysis (CoMFA) predicts that the large electrostatic field around the phosphate groups of ATP plays a crucial role in stabilizing the open state of the cardiac ryanodine receptor (RyR) channel. We therefore investigated the effects of adenosine-5'-(beta,gamma-methylenetriphosphate) (AMP-P...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：10.1124/mol.63.1.174

    authors： Chan WM,Welch W,Sitsapesan R

    更新日期：2003-01-01 00:00:00

  • Ceramide negatively regulates glutathione S-transferase gene transactivation via repression of hepatic nuclear factor-1 that is degraded by the ubiquitin proteasome system.

    abstract：:The level of cellular ceramide, an apoptotic rheostat, is increased by sphingomyelinase or de novo synthesis. The expression of the glutathione S-transferase (GST) gene, whose induction accounts for cell viability, is regulated by activation of CCAAT/enhancer binding protein-beta (C/EBPbeta) and NF-E2-related factor-2...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：10.1124/mol.65.6.1475

    authors： Park IN,Cho IJ,Kim SG

    更新日期：2004-06-01 00:00:00

  • Effects of beta-naphthoflavone on insulin receptor binding and protein kinase activity in rat liver and placenta.

    abstract：:Studies investigating the effects of beta-naphthoflavone (beta NF) on insulin receptor binding and its intrinsic protein kinase activity in rat liver and placenta were performed. Membranes were prepared from maternal liver and placenta on gestation day 11 and used for [125I]insulin radioreceptor assay. Scatchard analy...

    journal_title：Molecular pharmacology

    pub_type： 杂志文章

    doi：

    authors： Wang SL,Raizada MK,Shiverick KT

    更新日期：1988-03-01 00:00:00