Abstract：
OBJECTIVE:The aim of this study was to investigate the effects of hypo-osmotically induced calcium (Ca(2+)) transients on the organization of the actin cytoskeleton in articular chondrocytes. The secondary hypothesis tested was that actin restructuring following hypo-osmotic stress is mediated by gelsolin. METHODS:Isolated porcine chondrocytes were exposed to hypo-osmotic stress, and [Ca(2+)](i)was monitored using laser scanning microscopy. Calcium transients were monitored using fluorescent ratiometric imaging. The intracellular distribution of actin was examined using fluorescent immunohistochemistry and transient transfection with the pEGFP-actin plasmid. The intracellular distribution of gelsolin was investigated using fluorescent immunohistochemistry. RESULTS:Osmotic stress induced transient increases in [Ca(2+)](i)caused reorganization of intracellular actin through a mechanism that required Ca(2+)in the extracellular media. Fluorescence microscopy revealed that gelsolin was colocalized with F-actin immediately following hypo-osmotic stress but dissociated over time. CONCLUSION:These results indicate that hypo-osmotic stress induces a gelsolin-mediated reorganization of actin through a transient increase in [Ca(2+)](i).
journal_name
Osteoarthritis Cartilagejournal_title
Osteoarthritis and cartilageauthors
Erickson GR,Northrup DL,Guilak Fdoi
10.1053/s1063-4584(02)00347-3keywords:
subject
Has Abstractpub_date
2003-03-01 00:00:00pages
187-97issue
3eissn
1063-4584issn
1522-9653pii
S1063458402003473journal_volume
11pub_type
杂志文章abstract：OBJECTIVE:Reliable evaluation of joint space width and subchondral sclerosis of osteoarthritic joints is difficult. The present study describes a new digital method to analyse standard radiographs of the ankle. DESIGN:Standardized radiographs were taken of the ankle of 12 patients with severe osteoarthritis (OA) under...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1053/joca.2000.0384
更新日期：2001-04-01 00:00:00
abstract：:Inappropriate biomechanics, namely wear-and-tear, has been long believed to be a main cause of osteoarthritis (OA). However, this view is now being re-evaluated, especially when examined alongside mechanobiology and new biomechanical studies. These are multiscale experimental and computational studies focussing on cel...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,评审
doi：10.1016/j.joca.2016.09.023
更新日期：2017-02-01 00:00:00
abstract：OBJECTIVE:To evaluate anterior cruciate ligament transection (ACLT) and destabilization of the medial meniscus (DMM) surgical instability models of osteoarthritis (OA) in the 129/SvEv mouse knee joint. DESIGN:Micro-surgical techniques were used to perform ACLT or DMM under direct visualization. Histological scoring wa...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2007.03.006
更新日期：2007-09-01 00:00:00
abstract：OBJECTIVE:Hip labrum pathology has only begun to emerge as a significant source of groin pain in the last decade since the development of hip arthroscopy. Few data are available on the anatomy, histology and function of this structure. Moreover, no metabolic data exist at cellular level. The aim of this study was to ch...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2012.03.023
更新日期：2012-07-01 00:00:00
abstract：OBJECTIVES:Curcumin (diferuloylmethane) is the principal biochemical component of the spice turmeric and has been shown to possess potent anti-catabolic, anti-inflammatory and antioxidant, properties. This article aims to provide a summary of the actions of curcumin on articular chondrocytes from the available literatu...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,评审
doi：10.1016/j.joca.2009.10.002
更新日期：2010-02-01 00:00:00
abstract：:Osteoarthritis (OA) is not caused by a simple consequence of aging and cartilage degradation. Based on the conventional paradigm, OA has been considered a degenerative joint disorder. However, the dominant clinical symptom has been characterized by a non-infectious chronic inflammatory condition with infiltration of i...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1053/joca.1998.0224
更新日期：1999-07-01 00:00:00
abstract：OBJECTIVE:Calcitonin (CT) has been recently shown to exhibit direct protective effects on articular cartilage against joint degenerative disease. It has been proposed that CT might act via the CT receptor (CTR) to activate the cyclic AMP (cAMP) pathway and protect type II collagen degradation. In this study, we investi...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2007.08.003
更新日期：2008-04-01 00:00:00
abstract：OBJECTIVE:Rat intervertebral disc (IVD) is one of the most commonly used and cost-effective alternative models for human IVD. Many IVD related clinical studies need to be pre-tested on rat IVDs. However, studies on the heterogeneous cell clusters of the rat IVD are inadequate, and a further understanding of the marker ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2020.12.005
更新日期：2020-12-15 00:00:00
abstract：OBJECTIVE:The aim of the present study was to determine if the electroporation-mediated gene transfer of SOX trio enhances the chondrogenic potential of adipose stem cells (ASCs). DESIGN:ASCs were transfected with SOX trio genes using an electroporation technique and cultured for 3 weeks. The pellets were analyzed for...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2011.01.005
更新日期：2011-04-01 00:00:00
abstract：INTRODUCTION:In clinical tissue-engineering-based approaches to articular cartilage repair, various types of flap are frequently used to retain an implanted construct within the defect, and they are usually affixed by suturing. We hypothesize that the suturing of articular cartilage is associated with a loss of chondro...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2008.01.009
更新日期：2008-09-01 00:00:00
abstract：:To highlight methodological challenges in the design and conduct of randomized trials of surgical interventions and to propose strategies for addressing these challenges. This paper focuses on three broad areas: enrollment; intervention; and assessment including implications for analysis. For each challenge raised in ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,评审
doi：10.1016/j.joca.2015.02.024
更新日期：2015-05-01 00:00:00
abstract：OBJECTIVE:Meniscal injuries can lead to mechanical overloading of articular cartilage and eventually to knee osteoarthritis. The objective was to evaluate the potential of contrast enhanced computed tomography (CECT) to image contrast agent (CA) diffusion in human menisci with a clinical cone beam CT scanner. DESIGN:I...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2015.03.037
更新日期：2015-08-01 00:00:00
abstract：OBJECTIVE:The goal of this study was to model the longitudinal progression of knee osteoarthritis (OA) and build a prognostic tool that uses data collected in 1 year to predict disease progression over 8 years. DESIGN:To model OA progression, we used a mixed-effects mixture model and 8-year data from the Osteoarthriti...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2018.08.003
更新日期：2018-12-01 00:00:00
abstract：OBJECTIVE:To define for the first time the transcriptomes of normal and end-stage osteoarthritis (OA) hip cartilage. MATERIALS AND METHODS:RNA was isolated from cartilage within 2h of joint replacement surgery. Gene expression was analyzed using Agilent GeneSpring GX 11 following hybridization to Illumina Human HT-12 ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2012.05.006
更新日期：2012-09-01 00:00:00
abstract：OBJECTIVE:This systematic literature review aimed to evaluate the use of conventional radiography (CR) in hand osteoarthritis (OA) and to assess the metric properties of the different radiographic scoring methods. DESIGN:Medical literature databases up to November 2013 were systematically reviewed for studies reportin...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,评审
doi：10.1016/j.joca.2014.05.026
更新日期：2014-10-01 00:00:00
abstract：OBJECTIVE:To evaluate the efficacy of a single intra-articular (IA) dose of FX006, an extended-release formulation of triamcinolone acetonide (TCA) in poly(lactic-co-glycolic acid) (PLGA) microspheres, on the sequelae of repeated episodes of synovitis. DESIGN:Three flares of localized synovitis in the right knee of ra...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2014.09.019
更新日期：2015-01-01 00:00:00
abstract：OBJECTIVE:Solutes and interstitial water are naturally transported from cartilage by load-induced interstitial fluid pressures. Fluid and solute recovery during joint articulation have been primarily attributed to passive diffusion and mechanical 'pumping' from dynamic loading. This paper tests if the sliding action of...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2017.08.014
更新日期：2017-12-01 00:00:00
abstract：OBJECTIVE:Adult articular cartilage is a physiologically hypoxic tissue with a proposed gradient of oxygen tension ranging from <10% oxygen at the cartilage surface to <1% in the deepest layers. This gradient may be disturbed during diseases of the joint, for example in rheumatoid arthritis when synovial fluid pO(2)fal...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1053/joca.2000.0396
更新日期：2001-05-01 00:00:00
abstract：OBJECTIVE:Here we investigate whether monolayer culture or culture at 21% oxygen influences activity of cytochrome c oxidase, the terminal enzyme in the respiratory chain whose activity is essential for oxidative metabolism and whether return to three dimensional (3-D) culture restores cytochrome c oxidase activity to ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2009.03.001
更新日期：2009-08-01 00:00:00
abstract：OBJECTIVE:To study the effect of 17β-estradiol (E2) and the superficial zone (SFZ) on cell death and proteoglycan degradation in articular cartilage after a single injurious compression in vitro. METHOD:Cartilage explants from the femoropatellar groove of 2 year old cows with or without the SFZ were cultured serum-fre...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2013.07.007
更新日期：2013-11-01 00:00:00
abstract：INTRODUCTION:Systematic reviews agree that knee osteoarthritis (OA) is related to occupational activities, but have not quantified the overall risks. METHODS:Systematic review of observational studies of knee OA and occupation. Job titles, elite sport, heavy work, kneeling, and other activities were included. Relative...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.joca.2011.02.016
更新日期：2011-07-01 00:00:00
abstract：BACKGROUND:The functional index for hand osteoarthritis (FIHOA), a 10-item investigator-administered questionnaire, was validated in 1995. It is relevant, reliable and has good external and internal validities. OBJECTIVE:To assess the sensitivity to change over time of the FIHOA. PATIENTS/METHODS: PATIENTS:symptomat...
journal_title：Osteoarthritis and cartilage
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1053/joca.2000.0332
更新日期：2000-01-01 00:00:00
abstract：OBJECTIVE:Abnormal metabolic activities of chondrocytes may cause articular cartilage (AC) degradation, but key transcription factors regulating metabolic activities in AC of aging individuals remain unknown. This study aimed to investigate the role of transcription factor NFAT1 in regulating the expression of anabolic...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2016.02.003
更新日期：2016-07-01 00:00:00
abstract：OBJECTIVE:Chondrocyte death, a notable feature of osteoarthritis, may play a role in the initiation of cartilage degeneration. The present study was aimed at uncovering the nature and involvement of cell death in the initiation of cartilage degeneration induced by mechanical trauma. METHODS:Articular cartilage discs o...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2006.03.012
更新日期：2006-10-01 00:00:00
abstract：OBJECTIVE:To date semiquantitative whole-organ scoring of knee osteoarthritis (OA) relies on 1.5 Tesla (T) Magnetic resonance imaging (MRI) systems. Less costly 1.0 T extremity systems have been introduced that offer superior patient comfort, but may have limitations concerning field-of-view and image quality. The aim ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,多中心研究
doi：10.1016/j.joca.2009.08.017
更新日期：2010-02-01 00:00:00
abstract：OBJECTIVE:Sensitive biomarkers are needed to understand synovial response to therapy in osteoarthritis (OA). Dynamic, contrast-enhanced magnetic resonance imaging (DCE MRI) provides quantitative, novel measures of synovial inflammation. This exploratory study examined DCE-assessed synovial response to intra-articular c...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2014.05.024
更新日期：2014-10-01 00:00:00
abstract：OBJECTIVE:Extracellular inorganic pyrophosphate (ePPi) plays a key role in the regulation of normal and pathologic mineralization. The purpose of this work was to evaluate the role of P1 and P2 purine receptors in modulating ePPi production by articular chondrocytes. METHODS:Porcine cartilage explants and chondrocyte ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2010.08.004
更新日期：2010-11-01 00:00:00
abstract：OBJECTIVE:Cam hip shape morphology is a recognised cause of femoroacetabular impingement (FAI) and is associated with hip osteoarthritis. Our aim was to systematically review the available epidemiological evidence assessing the prevalence of cam hip shape morphology in the general population and any studied subgroups i...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,评审
doi：10.1016/j.joca.2015.12.020
更新日期：2016-06-01 00:00:00
abstract：OBJECTIVE:To summarise important findings from biomarker studies relevant to osteoarthritis (OA), published between April 2016 and March 2017; to consider these findings in the context of new discoveries and technologies, and clinical and scientific need in OA. DESIGN:Studies were selected by PubMed search, conducted ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,评审
doi：10.1016/j.joca.2017.10.016
更新日期：2018-03-01 00:00:00
abstract：OBJECTIVE:Nerve growth factor (NGF) has a pivotal role in peripheral hyperalgesia and inflammation; anti-NGF antibodies attenuate pain responses in inflammatory pain models, and in people with osteoarthritis (OA) or low back pain. The aim of this study was to characterise the peripheral mechanisms contributing to the a...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2016.05.015
更新日期：2016-09-01 00:00:00
