FISH banding methods: applications in research and diagnostics.

abstract：:Differences in protein levels and the activation of signaling pathways have been extensively studied in tumor tissues, but the implementation of protein profiling methods in routine hospital workflows lags far behind that of nucleic acid-based approaches. In this review, major technologies that are currently used for ...

journal_title：Expert review of molecular diagnostics

authors： Boellner S,Becker KF

更新日期：2015-01-01 00:00:00

abstract：:With the emergence of new viral infections, it is necessary to set up new target-specific assays, based on existing molecular techniques such as real-time PCR, as quickly as possible. Without these diagnostic tools, the geographical spread of new infections, follow-up of the disease outbreak and analysis of the pathog...

journal_title：Expert review of molecular diagnostics

authors： Pas SD,Koopmans MP,Niedrig M

更新日期：2016-01-01 00:00:00

abstract：INTRODUCTION:Tuberculosis (TB) is a leading killer worldwide. End TB strategy aims at ending the TB epidemic by 2030. Early, accurate, and affordable diagnosis represents a cornerstone to achieve this goal. Innovative strategies for TB diagnostics have been introduced. However, the ideal assay is yet unavailable and co...

journal_title：Expert review of molecular diagnostics

authors： El-Samadony H,Althani A,Tageldin MA,Azzazy HME

更新日期：2017-05-01 00:00:00

abstract：INTRODUCTION:Syphilis remains an important and preventable cause of stillbirth and neonatal mortality. About 1 million women with active syphilis become pregnant each year. Without treatment, 25% of them will deliver a stillborn baby and 33% a low birth weight baby with an increased chance of dying in the first month o...

journal_title：Expert review of molecular diagnostics

authors： Marks M,Mabey DC

更新日期：2017-04-01 00:00:00

abstract：:Molecular genetic testing involves DNA analysis using various methods for the purpose of diagnosing genetic disorders. In the prenatal DNA diagnostic setting, fetal DNA is usually tested for a specific single-gene disorder for which the fetal risk is 25% or more. In contrast, cytogenetic testing is often used to detec...

journal_title：Expert review of molecular diagnostics

authors： Roa BB,Pulliam J,Eng CM,Cheung SW

更新日期：2005-11-01 00:00:00

abstract：:Stroke is a multifactorial disease responsible for nearly 10% of deaths each year in industrialized countries. While some monogenic forms of stroke have been described, the vast majority result from the common polygenic form of the disease. Progress in molecular genetics has allowed the identification, through genome-...

journal_title：Expert review of molecular diagnostics

authors： Domingues-Montanari S,Mendioroz M,del Rio-Espinola A,Fernández-Cadenas I,Montaner J

更新日期：2008-07-01 00:00:00

abstract：:Renal cell carcinoma is one of the common malignancies of the genitourinary tract. In approximately one third of patients, distant metastases are present at the time of initial diagnosis and in another third, the tumor will recur even after nephrectomy with a curative intent. Renal cell carcinoma is resistant to all c...

journal_title：Expert review of molecular diagnostics

authors： Mohammed A,Shergill I,Little B

更新日期：2009-01-01 00:00:00

abstract：:Although comprehensive molecular diagnostics and personalized medicine have sparked excitement among researchers and clinicians, they have yet to be fully incorporated into today's standard of care. This is despite the discovery of disease-related oncogenes, tumor-suppressor genes and protein biomarkers, as well as ot...

journal_title：Expert review of molecular diagnostics

authors： Zoon CK,Starker EQ,Wilson AM,Emmert-Buck MR,Libutti SK,Tangrea MA

更新日期：2009-07-01 00:00:00

abstract：INTRODUCTION:Fine needle aspiration (FNA) with cytologic examination remains the standard of care for investigation of thyroid nodules. However, as many as 30% of FNA samples are cytologically indeterminate for malignancy, which confounds clinical management. To reduce the burden of repeat diagnostic testing and unnece...

journal_title：Expert review of molecular diagnostics

authors： Moore MD,Panjwani S,Gray KD,Finnerty BM,Zarnegar R,Fahey TJ 3rd

更新日期：2017-06-01 00:00:00

abstract：:Concomitant advances made by the Human Genome Project and in the development of nucleic acid screening technologies are driving the expansion of pharmacogenomic research and molecular diagnostics. However, most current technologies are restrictive due to their complexity and/or cost, limiting the potential of personal...

journal_title：Expert review of molecular diagnostics

authors： de Arruda M,Lyamichev VI,Eis PS,Iszczyszyn W,Kwiatkowski RW,Law SM,Olson MC,Rasmussen EB

更新日期：2002-09-01 00:00:00

abstract：:The generation of a draft sequence of a the human genome has provided the opportunity to characterize human diversity, even as it pertains to differences in host response to parasitic infection with organisms that cause lymphatic filariasis, malaria and schistosomiasis. Worldwide, human infection with filarial pathoge...

journal_title：Expert review of molecular diagnostics

authors： Choi EH,Nutman TB,Chanock SJ

更新日期：2003-05-01 00:00:00

abstract：:Skin melanoma, a life-threatening disease, has a recently reported worldwide increase in incidence, despite primary prevention. Skin melanoma statistics emphasize the need for finding markers related to the immune response of the host. The mechanisms that are able to over-power the local immune surveillance comprise m...

journal_title：Expert review of molecular diagnostics

authors： Neagu M,Constantin C,Tanase C

更新日期：2010-10-01 00:00:00

abstract：:It is becoming increasingly clear that the herpes simplex viruses (HSVs) 1 and 2 constitute a major, global, public health problem, particularly as genital herpes is implicated in the causation of a significant percentage of onwards transmission of the HIV virus. A major factor in the transmission of HSV is that most ...

journal_title：Expert review of molecular diagnostics

authors： Ryan C,Kinghorn G

更新日期：2006-09-01 00:00:00

abstract：:In recent years, the real time-based TaqMan(®) technology has allowed the development of highly sensitive hepatitis viral load tests with broad dynamic ranges. The increasing applications of these tests in clinical diagnostics have shown the utility of viral load as a predictive marker of treatment response and proven...

journal_title：Expert review of molecular diagnostics

authors： Colucci G,Knobel R

更新日期：2011-11-01 00:00:00

abstract：:Trypanosoma cruzi is the etiologic agent of Chagas' disease, a chronic inflammatory condition that results in heart and digestive complications. The first draft of the parasite genome is now complete and it is expected that, along with the published genomic and proteomic analyses discussed herein, it will lead to the ...

journal_title：Expert review of molecular diagnostics

authors： Huete-Pérez JA,Flores-Obando RE,Ghedin E,Caffrey CR

更新日期：2005-07-01 00:00:00

abstract：:The fundamental strategy of the current postgenomic era or the era of functional genomics is to expand the scale of biologic research from studying single genes or proteins to studying all genes or proteins simultaneously using a systematic approach. As recently developed methods for obtaining genome-wide mRNA express...

journal_title：Expert review of molecular diagnostics

authors： Ewis AA,Zhelev Z,Bakalova R,Fukuoka S,Shinohara Y,Ishikawa M,Baba Y

更新日期：2005-05-01 00:00:00

abstract：:Alzheimer's disease is the most common form of dementia in the elderly and its prevalence is rapidly rising. Although there is no cure for Alzheimer's disease, treatment can be administered to slow progression or delay the onset of symptoms. A major challenge is the early identification of patients who will develop Al...

journal_title：Expert review of molecular diagnostics

authors： Chang CY,Silverman DH

更新日期：2004-01-01 00:00:00

abstract：INTRODUCTION:Bodily fluids like serum and plasma contain significant amounts of tumor-derived circulating cell-free RNA, which holds the potential to serve as diagnostic biomarker. Consequently, liquid biopsies comprising circulating cell-free RNA might help to facilitate personalized treatment strategies for patients ...

journal_title：Expert review of molecular diagnostics

authors： Ellinger J,Gevensleben H,Müller SC,Dietrich D

更新日期：2016-10-01 00:00:00

abstract：INTRODUCTION:Bipolar disorder is a chronic and disabling mood disorder with a complex pathophysiological basis. A significant percentage of patients do not receive correct diagnosis which directly influences therapeutic response, rendering recovery troublesome. There is a long-standing need for proper non-clinically ba...

journal_title：Expert review of molecular diagnostics

authors： Teixeira AL,Salem H,Frey BN,Barbosa IG,Machado-Vieira R

更新日期：2016-11-01 00:00:00

abstract：:Colorectal cancer (CRC) is a slow-developing cancer (10-15 years) with one of the highest frequencies in the world's population. Many countries have implemented various CRC screening programs, but have not achieved the desired compliance. Colonoscopy - considered the gold standard for CRC screening - has its limitatio...

journal_title：Expert review of molecular diagnostics

authors： Molnár B,Tóth K,Barták BK,Tulassay Z

更新日期：2015-02-01 00:00:00

abstract：INTRODUCTION:A reliable liver autoimmune serology for the diagnosis of primary biliary cholangitis (PBC) is of particular importance. Recognition of patients at early stages and prompt treatment initiation may alter the outcome, slow progression, delays liver failure, and improves survival. AREAS COVERED:In this revie...

journal_title：Expert review of molecular diagnostics

authors： Gatselis NK,Dalekos GN

更新日期：2016-09-01 00:00:00

abstract：:The emergence of drug resistance remains one of the most challenging issues in the treatment of HIV-1 infection. The extreme replication dynamics of HIV facilitates its escape from the selective pressure exerted by the human immune system and by the applied combination drug therapy. This article reviews computational ...

journal_title：Expert review of molecular diagnostics

authors： Cordes F,Kaiser R,Selbig J

更新日期：2006-03-01 00:00:00

abstract：INTRODUCTION:Familial hypercholesterolemia (FH) is a common genetic cause of premature coronary heart disease that is widely underdiagnosed and undertreated. To improve the identification of FH and initiate timely and appropriate treatment strategies, genetic testing is becoming increasingly offered worldwide as a cent...

journal_title：Expert review of molecular diagnostics

authors： Iacocca MA,Hegele RA

更新日期：2017-07-01 00:00:00

abstract：:Lipoprotein-associated phospholipase (Lp-PL)A2 is a recently described and potentially useful plasma biomarker associated with cardiovascular disease. The enzyme, originally named platelet-activating factor acetylhydrolase (PAF-AH), has two prominent biological activities. First, it inactivates the prominent proinflam...

journal_title：Expert review of molecular diagnostics

authors： Carlquist JF,Muhlestein JB,Anderson JL

更新日期：2007-09-01 00:00:00

abstract：:Circulating tumor cells (CTCs) are increasingly recognized for their potential utility in cancer diagnosis and prognosis. Emerging technologies in the past decade have allowed possible isolation and characterization of CTCs in the peripheral blood of cancer patients, including immunomagnetic technique coupled with imm...

journal_title：Expert review of molecular diagnostics

authors： Cho WC

更新日期：2014-03-01 00:00:00

abstract：:Existing and future diagnostic technologies are providing a huge amount of data about the patient, which are to be sensibly managed by the treating physician. More than that, the sheer complexity of the interrelation between these data are calling for a radically new approach in the handling of medical data and inform...

journal_title：Expert review of molecular diagnostics

authors： Fierz W

更新日期：2002-07-01 00:00:00

abstract：INTRODUCTION:Rapidity, simplicity, and portability are highly desirable characteristics of tests and devices designed for performing diagnostics at the point of care (POC), either near patients managed in healthcare facilities or to offer bioanalytical alternatives in external settings. By reducing the turnaround time ...

journal_title：Expert review of molecular diagnostics

authors： Bissonnette L,Bergeron MG

更新日期：2017-05-01 00:00:00

abstract：INTRODUCTION:Preimplantation genetic diagnosis (PGD) was introduced as an alternative to prenatal diagnosis: embryos cultured in vitro were analysed for a monogenic disease and only disease-free embryos were transferred to the mother, to avoid the termination of pregnancy with an affected foetus. It soon transpired tha...

journal_title：Expert review of molecular diagnostics

authors： Sermon K

更新日期：2017-01-01 00:00:00

abstract：:A concise survey of the emerging PD-loop technology is presented, which outlines several exemplary methods with robust DNA diagnostic potential: duplex DNA capture, topological DNA labeling, nondenaturing DNA sequencing and hybridization of molecular beacons to double-stranded DNA. Advantages of these new PNA-based as...

journal_title：Expert review of molecular diagnostics

authors： Demidov VV

更新日期：2001-09-01 00:00:00

abstract：:Rheumatoid arthritis (RA) is a systemic, chronic and inflammatory disease of unknown etiology. HLA-DRB1 and PTPN22 1858T gene variants are risk factors of RA, clinical manifestations and rate of progression of joint destruction in this autoimmune disease. Currently, several immunopathogenetic models of other genes (CT...

journal_title：Expert review of molecular diagnostics

authors： Nagy ZB,Csanád M,Tóth K,Börzsönyi B,Demendi C,Rigó J Jr,Joó JG

更新日期：2010-07-01 00:00:00