Abstract:
AIM:Several amplification and detection formats for the analysis of short tandem repeat loci are readily available to the forensic laboratory. Careful consideration must be given to the throughput, sensitivity, concordance, data interpretation, facility requirements, and costs of operation. The Pennsylvania State Police DNA Laboratory sought to establish that of any of the amplification or detection formats generally used in the United States generates concordant results and that the use of several formats within one laboratory provides a solution to the interpretation of difficult evidentiary samples. METHODS:Validation work consisting of sensitivity, precision, mixture, and substrate studies was performed by use of each of three detection formats (ABI Prism(r)310 Genetic Analyzer, ABI Prism(r)377 DNA Sequencer, and the Hitachi FMBIO(r)II Fluorescent Scanner) and three amplification systems (GenePrint(r) PowerPlex 16, GenePrint(r) PowerPlex 1.1/2.1, and AmpflSTR ProfilerPlus/COfiler). The results generated in each of the formats were compared, along with the problems incurred. RESULTS:All allele calls were concordant, with the exception of primer region variants, and all detection systems were sensitive and reliable. Even with the use of multiple formats, a general protocol can be written with only one set of interpretation guidelines. CONCLUSION:National databases can be used with input data from any of these formats. The use of several detection formats allowed the forensic scientist to select a system, based on sample quality, quantity, and throughput requirements. Interpretation issues resulting from complex mixtures, degraded samples, rare microvariants, internal primer variants, unusual heterozygote ratios, above or below ladder alleles, and potential tri-alleles can be verified.
journal_name
Croat Med Jjournal_title
Croatian medical journalauthors
Tomsey CS,Kurtz M,Kist F,Hockensmith M,Call Pkeywords:
subject
Has Abstractpub_date
2001-06-01 00:00:00pages
239-43issue
3eissn
0353-9504issn
1332-8166journal_volume
42pub_type
杂志文章abstract::Hereditary factor XI (FXI) deficiency is a mild bleeding disorder, rare in the general population but relatively common among Ashkenazi Jews. The human F11 gene comprises 15 exons, spanning over 23 kb of the long arm of chromosome 4 (4q35). Homozygotes or compound heterozygotes typically show severe FXI deficiency, wh...
journal_title:Croatian medical journal
pub_type:
doi:
更新日期:2020-02-29 00:00:00
abstract:AIM:To compare the health-related quality of life of unemployed and employed women with disabilities and establish factors affecting their life satisfaction. METHODS:The study included 318 women with disabilities, 160 of whom were employed and 158 unemployed, paired according to age and region of residence. The health...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2011.52.550
更新日期:2011-08-15 00:00:00
abstract:AIM:To test antinociceptive properties of botulinum toxin type A (BTX-A) in rats with carrageenan- and capsaicin-induced pain and inflammation. METHODS:Pain was provoked with carrageenan (1%) or capsaicin (0.1%) injection into the plantar surface of the rat paw-pad. The effect of BTX-A 5 U/kg on carrageenan- and capsa...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2005-04-01 00:00:00
abstract:AIM:To detect polymorphisms of 23 Y-chromosomal short tandem repeat (STR) loci, including 6 new loci, in a reference database of male population of Bosnia and Herzegovina, as well as to assess the importance of increasing the number of Y-STR loci utilized in forensic DNA analysis. METHODS:The reference sample consiste...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2013.54.286
更新日期:2013-06-01 00:00:00
abstract::Susac syndrome is a rare microangiopathy of cochlea, retina, and brain. We report a case of a 30-year-old man with Susac syndrome. The patient initially suffered from unilateral hearing loss associated with peripheral vestibular syndrome, and followed with recurrent arterial retinal occlusions and encephalopathy. The ...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2004-06-01 00:00:00
abstract:AIM:To determine the human Y-chromosome haplogroup backgrounds of intermediate-sized variant alleles displayed by short tandem repeat (STR) loci DYS392, DYS449, and DYS385, and to evaluate the potential of each intermediate variant to elucidate new phylogenetic substructure within the human Y-chromosome haplogroup tree...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2009.50.239
更新日期:2009-06-01 00:00:00
abstract:AIM:Oncostatin-M, a member of the gp 130 family of cytokines, has been associated with inflammation, connective tissue production, and extracellular matrix turnover. Since the reperfused heart is associated with an intense inflammatory reaction followed by scar formation, we tested the hypothesis that oncostatin-M is u...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2004-04-01 00:00:00
abstract:AIM:To evaluate the efficacy and toxicity of preoperative chemoradiotherapy with capecitabine in locally advanced rectal cancer. METHODS:Between June 2004 and January 2005, 57 patients with operable, clinical stage II-III adenocarcinoma of the rectum entered the prospective phase II study. Radiation dose was 45 Gy (25...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2006-10-01 00:00:00
abstract:: ...
journal_title:Croatian medical journal
pub_type: 社论,历史文章
doi:
更新日期:2018-10-31 00:00:00
abstract:AIM:To assess diseases outcomes and tolerability of real-life second-line nivolumab in a series of metastatic renal cell carcinoma (mRCC) patients. METHODS:This retrospective chart review involved prospectively monitored patients (named patient program) treated with second-line nivolumab for mRCC at the University Hos...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2020-08-31 00:00:00
abstract:AIM:To analyze the haplotype of the Ezhava population of Kerala, south India, using 8 short tandem repeat (STR) loci on the Y chromosome and trace the paternal genetic lineage of the population. METHODS:Whole blood samples (n=104) were collected from unrelated healthy men of the Ezhava population over a period of one ...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2011.52.344
更新日期:2011-06-01 00:00:00
abstract::The aim of this article is to offer a concise interpretation of the scientific data about the topic of Croatian genetic heritage that was obtained over the past 10 years. We made a short overview of previously published articles by our and other groups, based mostly on Y-chromosome results. The data demonstrate that C...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2011.52.225
更新日期:2011-06-01 00:00:00
abstract:AIM:To evaluate scientific production among research fellows employed at the Zagreb University School of Medicine and identify factors associated with their scientific output. METHOD:We conducted a survey among research fellows and their mentors during June 2005. The main outcome measure was publication success, defin...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2006-10-01 00:00:00
abstract::We report on the 10-year follow-up and clinical, cytogenetic, and molecular investigation of a girl admitted for evaluation because of speech delay, learning difficulties, aggressive behavior, and dysmorphic facial features that included high forehead, round face, epicanthic folds, low-set dysplastic ears, flat nasal ...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2011.52.415
更新日期:2011-06-01 00:00:00
abstract:AIM:To investigate the correlation between the length of the title of a scientific article and the number of citations it receives, in view of the common editorial call for shorter titles. METHODS:Title and the number of citations to all articles published in 2005 in 22 arbitrarily chosen English-language journals (n=...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2010.51.165
更新日期:2010-04-01 00:00:00
abstract:AIM:To immunohistochemically evaluate the expression of MAGE-A1, MAGE-A, and NY-ESO-1 cancer/testis (C/T) tumor antigens in medullary breast cancer (MBC) tumor samples and to analyze it in relation to the clinicopathological features. METHODS:This retrospective study included samples from 49 patients: 40 with typical ...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2011.52.171
更新日期:2011-04-15 00:00:00
abstract:AIM:To investigate breastfeeding practices among mothers of infants in the city of Mostar, Bosnia and Herzegovina. METHODS:The cross-sectional self-report study was conducted among 326 mothers of infants visiting the Advisory Center for Infants at the Medical Center in Mostar and at the Children's Department of Mostar...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2004-02-01 00:00:00
abstract::To examine ways of ensuring access to health services within the framework of primary health care (PHC), since the goal of PHC to make universal health care available to all people has become increasingly neglected amid emerging themes of globalization, trade, and foreign policy. From a public health point of view, we...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/2010.51.181
更新日期:2010-06-01 00:00:00
abstract:AIM:To assess the influence of depression on patients' satisfaction with lumbar discectomy performed by two different surgical techniques. METHODS:A prospective matched-cohort analysis of classical lumbar discectomy following static imaging (n = 45) and microlumbar "key-hole" discectomy after dynamic CT/myelography (n...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2002-12-01 00:00:00
abstract:AIM:To explore the economic justification for introducing ultrasound screening for developmental dysplasia of the hip in Croatia. METHODS:The analysis was based on the two formulas: that cost-benefit equals benefit/cost, and that net benefit equals benefit minus cost. Screening costs were expressed as a sum of trainin...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2001-04-01 00:00:00
abstract:AIM:To estimate the incidence of postural orthostatic tachycardia syndrome (POTS) in the population of Zagreb, Croatia, and to determine the patients' demographic and clinical characteristics. METHODS:From 2012-2017, we identified patients with POTS by a retrospective analysis of medical records at University Hospital...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2020-10-31 00:00:00
abstract:AIM:To determine the association of carotid atherosclerosis and right ventricular diastolic dysfunction (DD) among treated hypertensive Nigerian patients. METHODS:This was a single center cross-sectional study performed at the Cardiology Clinic of LAUTECH Teaching Hospital, Ogbomoso, Nigeria between January and Decemb...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2013.54.555
更新日期:2013-12-01 00:00:00
abstract::The attainment of the fifth Millennium Development Goal requires adequate national reserves of skilled birth attendants. Nurses, midwives, and their equivalents form the frontline of the formal health system are a critical element of global efforts to reduce ill-health and poverty in the poorest areas of the world. Pl...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2008.3.318
更新日期:2008-06-01 00:00:00
abstract:AIM:To assess long-term mental health outcomes in people who suffer from war-related posttraumatic stress disorder (PTSD) but do not receive appropriate treatment. METHODS:We interviewed 264 subjects from former Yugoslavia, who lived in Croatia, Serbia, Germany, and the United Kingdom. All of them had suffered from PT...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2009.50.465
更新日期:2009-10-01 00:00:00
abstract:AIM:To determine whether the students enrolled in the computer-based teaching program would take the final examination in pathology earlier than those who studied according to the previous traditional program. METHODS:The study included all medical students enrolled in the pathology course at the Zagreb University Sch...
journal_title:Croatian medical journal
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2005-06-01 00:00:00
abstract::Today the development of a unique professional language and publishing of professional and scientific publications is the basis of every profession, including the nursing profession. The task of the unique language specific to the nursing profession is to describe the nursing profession (to make it more familiar to th...
journal_title:Croatian medical journal
pub_type: 历史文章,杂志文章
doi:10.3325/cmj.2012.53.631
更新日期:2012-12-01 00:00:00
abstract:AIM:To evaluate the administration of antipyretics to children with upper respiratory tract infections (URTI) by their parents or guardians without consultation with physicians, and compare epidemiological and clinical characteristics of patients who received antipyretics and of untreated patients. METHODS:A prospecti...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2011.52.48
更新日期:2011-02-01 00:00:00
abstract:AIM:To investigate whether socioeconomic inequalities at a micro-scale, through their effect on major health risk factors and other health indicators, contribute to health status in an isolated island population with demonstrated reduced genetic and environmental variability. METHODS:This cross-sectional study was per...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2007-10-01 00:00:00
abstract:AIM:To investigate what Zagreb University students think about professor-student dating. METHODS:We surveyed 500 students from ten different Zagreb University schools using anonymous questionnaires. Twenty-five female and 25 male students from each school were asked to assess professor-student dating as either accepta...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2001-02-01 00:00:00
abstract::We present a paternity testing case in which a double incompatibility was found for two short tandem repeat (STR) markers, human fibrinogen alpha (FGA) and Penta E. Analysis of the trio (mother, father, and daughter) included the amplification with a battery of 15 autosomal short tandem repeats (STR) by using a commer...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2003-06-01 00:00:00