Differential melting of the transcription start site associated with changes in RNA polymerase-promoter contacts in initiating transcription complexes.

abstract：:Mannan-binding lectin (MBL) is the archetypical pathogen recognition molecule of the innate immune defense. Upon binding to microorganisms, reactions leading to the destruction of the offender ensue. MBL is an oligomer of structural subunits each composed of three identical polypeptides. We used atomic force microscop...

journal_title：Journal of molecular biology

authors： Jensenius H,Klein DC,van Hecke M,Oosterkamp TH,Schmidt T,Jensenius JC

更新日期：2009-08-07 00:00:00

abstract：:In order to develop a system which allows infection by an epitope-specific phage-antibody via an F-pilus expressing that epitope, a study on the expression of foreign sequences on F-pilin was undertaken. Initially, a plasmid library was constructed with random sequences encoding one to five amino acid residues fused t...

journal_title：Journal of molecular biology

authors： Rondot S,Anthony KG,Dübel S,Ida N,Wiemann S,Beyreuther K,Frost LS,Little M,Breitling F

更新日期：1998-06-12 00:00:00

abstract：:Carbon catabolite repression (CCR) of several operons in Bacillus subtilis and Bacillus megaterium is mediated by the cis-acting cre sequence and trans-acting catabolite control protein (CcpA). We describe purification of CcpA from B. megaterium and its interaction with regulatory sequences from the xyl operon. Specif...

journal_title：Journal of molecular biology

authors： Gösseringer R,Küster E,Galinier A,Deutscher J,Hillen W

更新日期：1997-03-07 00:00:00

abstract：:The 8-17 deoxyribozyme is an in vitro selected enzyme capable of sequence-specific cleavage of RNA. While selected to be a magnesium and zinc-utilizing enzyme, the 8-17 DNAzyme has been shown to utilize lead for its catalysis. Fluorescence-based experiments have indicated that the magnesium- and zinc-utilizing version...

journal_title：Journal of molecular biology

authors： Liu Y,Sen D

更新日期：2010-01-15 00:00:00

abstract：:Tau displacement from microtubules is the first step in the onset of tauopathies and is followed by toxic protein aggregation. However, other non-canonical functions of Tau might have a role in these pathologies. Here, we demonstrate that a small amount of Tau localizes in the nuclear compartment and accumulates in bo...

journal_title：Journal of molecular biology

authors： Siano G,Varisco M,Caiazza MC,Quercioli V,Mainardi M,Ippolito C,Cattaneo A,Di Primio C

更新日期：2019-02-15 00:00:00

abstract：:The secondary structure of ribonuclease T1 (RNase T1) in aqueous solution and its temperature-induced structural changes have been investigated by Fourier-transform infrared (FT-IR) spectroscopy. 13 to 14% alpha-helix and 32 to 33% beta-sheet were estimated from the resolution-enhanced FT-IR spectra, in agreement with...

journal_title：Journal of molecular biology

authors： Fabian H,Schultz C,Naumann D,Landt O,Hahn U,Saenger W

更新日期：1993-08-05 00:00:00

abstract：:The prokaryotic transcription factors GreA and GreB are involved in the regulation of transcript elongation by RNA polymerase (RNAP). Their known activities include suppression of transcription arrest, enhancement of transcription fidelity, and facilitation of the transition from abortive initiation to productive elon...

journal_title：Journal of molecular biology

authors： Koulich D,Nikiforov V,Borukhov S

更新日期：1998-02-20 00:00:00

abstract：:The intracellular innate antiviral response in human cells is an essential component of immunity against virus infection. As obligate intracellular parasites, all viruses must evade the actions of the host cell's innate immune response in order to replicate and persist. Innate immunity is induced when pathogen recogni...

journal_title：Journal of molecular biology

authors： Rustagi A,Gale M Jr

更新日期：2014-03-20 00:00:00

abstract：:Protein allostery plays a pivotal role in many regulatory processes. Prominent examples are cell-surface receptors, which allosterically transmit ligand-generated signals to their cytoplasmic domains, or bacterial transcription factors, which alternate between a free conformation and a DNA-bound conformation in respon...

journal_title：Journal of molecular biology

authors： Goeke D,Kaspar D,Stoeckle C,Grubmüller S,Berens C,Klotzsche M,Hillen W

更新日期：2012-02-10 00:00:00

abstract：:We have mapped the origin of DNA replication (oriB) downstream of the 23 S rRNA gene in each copy of the inverted repeat (IR) of tobacco chloroplast DNA between positions 130,502 and 131,924 (IR(A)) by a combination of approaches. In vivo chloroplast DNA replication intermediates were examined by two-dimensional agaro...

journal_title：Journal of molecular biology

authors： Kunnimalaiyaan M,Shi F,Nielsen BL

更新日期：1997-05-02 00:00:00

abstract：:The recent growth in protein databases has revealed the functional diversity of many protein superfamilies. We have assessed the functional variation of homologous enzyme superfamilies containing two or more enzymes, as defined by the CATH protein structure classification, by way of the Enzyme Commission (EC) scheme. ...

journal_title：Journal of molecular biology

authors： Todd AE,Orengo CA,Thornton JM

更新日期：2001-04-06 00:00:00

abstract：:Although numerous measurements of amyloid assembly of different proteins under distinct conditions in vitro have been performed, the molecular mechanisms underlying the specific self-association of proteins into amyloid fibrils remain obscure. Elucidating the nature of the events that initiate amyloid formation remain...

journal_title：Journal of molecular biology

authors： Eichner T,Radford SE

更新日期：2009-03-13 00:00:00

abstract：:The site-specific recombinases Flp and R from Saccharomyces cerevisiae and Zygosaccharomyces rouxii, respectively, are related proteins that share approximately 30% amino acid matches. They exhibit a common reaction mechanism that appears to be conserved within the larger Integrase family of site-specific recombinases...

journal_title：Journal of molecular biology

authors： Lee J,Serre MC,Yang SH,Whang I,Araki H,Oshima Y,Jayaram M

更新日期：1992-12-20 00:00:00

abstract：:Angiotensin I-converting enzyme (ACE), one of the central components of the renin-angiotensin system, is a key therapeutic target for the treatment of hypertension and cardiovascular disorders. Human somatic ACE (sACE) has two homologous domains (N and C). The N- and C-domain catalytic sites have different activities ...

journal_title：Journal of molecular biology

authors： Akif M,Georgiadis D,Mahajan A,Dive V,Sturrock ED,Isaac RE,Acharya KR

更新日期：2010-07-16 00:00:00

abstract：:Transfer RNA su7 G36 is a derivative of tRNA(Trp) with a 3'GUC anticodon complementary to the glutamine codon CAG. This tRNA requires a normally forbidden G-U wobble at the first codon position to suppress a UAG (amber) termination codon. Measurement of amber suppression by mutated su7 G36 tRNAs and correction for tRN...

journal_title：Journal of molecular biology

authors： Schultz DW,Yarus M

更新日期：1994-02-04 00:00:00

abstract：:Structure-based drug design is underway to inhibit the S100B-p53 interaction as a strategy for treating malignant melanoma. X-ray crystallography was used here to characterize an interaction between Ca(2)(+)-S100B and TRTK-12, a target that binds to the p53-binding site on S100B. The structures of Ca(2+)-S100B (1.5-A ...

journal_title：Journal of molecular biology

authors： Charpentier TH,Thompson LE,Liriano MA,Varney KM,Wilder PT,Pozharski E,Toth EA,Weber DJ

更新日期：2010-03-12 00:00:00

abstract：:When Escherichia coli cells lysogenic for bacteriophage lambda are induced with ultraviolet light, cells carrying cryptic lambda prophages are occasionally found among the apparently cured survivors. The lambda variant crypticogen (lambda crg) carries an insertion of the transposable element IS2, which increases the f...

journal_title：Journal of molecular biology

authors： Redfield RJ,Campbell AM

更新日期：1987-12-05 00:00:00

abstract：:The genome sciences face the challenge to characterize structure and function of a vast number of novel genes. Sequence search techniques are used to infer functional and structural information from similarities to experimentally characterized genes or proteins. The persistent goal is to refine these techniques and to...

journal_title：Journal of molecular biology

authors： Koppensteiner WA,Lackner P,Wiederstein M,Sippl MJ

更新日期：2000-03-03 00:00:00

abstract：:The folding pattern of the alpha-crystallin domain, a conserved protein module encoding the molecular determinants of structure and function in the small heat-shock protein superfamily, was determined in the context of the lens protein alphaA-crystallin by systematic application of site-directed spin labeling. The seq...

journal_title：Journal of molecular biology

authors： Koteiche HA,Mchaourab HS

更新日期：1999-11-26 00:00:00

abstract：:Rvb1 and Rvb2 are essential AAA(+) (ATPases associated with diverse cellular activities) helicases, which are important components of critical complexes such as chromatin remodeling and telomerase complexes. The oligomeric state of the Rvb proteins has been controversial. Independent studies from several groups have d...

journal_title：Journal of molecular biology

authors： Cheung KL,Huen J,Kakihara Y,Houry WA,Ortega J

更新日期：2010-12-03 00:00:00

abstract：:Previous work has shown that the α-tocopherol transfer protein (α-TTP) can bind to vesicular or immobilized phospholipid membranes. Revealing the molecular mechanisms by which α-TTP associates with membranes is thought to be critical to understanding its function and role in the secretion of tocopherol from hepatocyte...

journal_title：Journal of molecular biology

authors： Zhang WX,Thakur V,Lomize A,Pogozheva I,Panagabko C,Cecchini M,Baptist M,Morley S,Manor D,Atkinson J

更新日期：2011-01-28 00:00:00

abstract：:Hookworms are human parasites that have devastating effects on global health, particularly in underdeveloped countries. Ancylostoma ceylanicum infects humans and animals, making it a useful model organism to study disease pathogenesis. A. ceylanicum excretory-secretory protein 2 (AceES-2), a highly immunoreactive mole...

journal_title：Journal of molecular biology

authors： Kucera K,Harrison LM,Cappello M,Modis Y

更新日期：2011-04-22 00:00:00

abstract：:Loss-of-function mutations in the gene encoding the multifunctional protein, DJ-1, have been implicated in the pathogenesis of early-onset familial Parkinson's disease (PD), suggesting that DJ-1 may act as a neuroprotectant for dopaminergic (DA) neurons. Enhanced autophagy may benefit PD by clearing damaged organelles...

journal_title：Journal of molecular biology

authors： Gao H,Yang W,Qi Z,Lu L,Duan C,Zhao C,Yang H

更新日期：2012-10-19 00:00:00

abstract：:The RhaS and RhaR regulatory proteins are encoded in the Escherichia coli L-rhamnose gene cluster. We used complementation analysis and DNA mobility shift assays to show that RhaR is not the direct activator of the L-rhamnose catabolic operon, rhaBAD. An in-frame deletion of rhaS (rhaS-rhaR+) eliminated expression fro...

journal_title：Journal of molecular biology

authors： Egan SM,Schleif RF

更新日期：1993-11-05 00:00:00

abstract：:Despite proline being assumed to be a helix-breaker, a large number of alpha-helices are found to contain Pro in globular as well as membrane proteins. Proline has no free NH group and therefore cannot form the conventional intra-helical NH.O=C hydrogen bond. An analysis of known protein structures has shown that the ...

journal_title：Journal of molecular biology

authors： Chakrabarti P,Chakrabarti S

更新日期：1998-12-11 00:00:00

abstract：:A method using binding site "neighbor-effect" parameters (NEPs) is introduced to evaluate the effects of interaction between adjacent ligands on their binding to an infinite linear lattice. Binding site overlap is also taken into account. This enables the conditional probability approach of McGhee & von Hippel to be e...

journal_title：Journal of molecular biology

authors： Wolfe AR,Meehan T

更新日期：1992-02-20 00:00:00

abstract：:The biogenesis of the bacterial flagellum and chemotaxis apparatus in both Escherichia coli and Caulobacter crescentus requires the ordered expression of over 40 genes whose expression is controlled by a trans-acting regulatory hierarchy. In C. crescentus, additional control mechanisms ensure that the transcription of...

journal_title：Journal of molecular biology

authors： Kaplan JB,Dingwall A,Bryan R,Champer R,Shapiro L

更新日期：1989-01-05 00:00:00

abstract：:SnRK [SNF1 (sucrose non-fermenting-1)-related protein kinase] 2.6 [open stomata 1 (OST1)] is well characterized at molecular and physiological levels to control stomata closure in response to water-deficit stress. OST1 is a member of a family of 10 protein kinases from Arabidopsis thaliana (SnRK2) that integrates absc...

journal_title：Journal of molecular biology

authors： Yunta C,Martínez-Ripoll M,Zhu JK,Albert A

更新日期：2011-11-18 00:00:00

abstract：:Understanding the interaction of Arf and Hdm2 has recently become a central issue in cancer biology. In response to hyperproliferative signals, p14(Arf) stabilizes p53 by binding to Hdm2 and inhibits the ubiquitination and subsequent proteosome-dependent degradation of p53. The medical importance of the Arf-Hdm2-p53 r...

journal_title：Journal of molecular biology

authors： Bothner B,Lewis WS,DiGiammarino EL,Weber JD,Bothner SJ,Kriwacki RW

更新日期：2001-11-23 00:00:00

abstract：:Riboswitches are complex folded RNA domains found in noncoding regions of mRNA that regulate gene expression upon small molecule binding. Recently, Breaker and coworkers reported a tandem aptamer riboswitch (VCI-II) that binds glycine cooperatively. Here, we use hydroxyl radical footprinting and small-angle X-ray scat...

journal_title：Journal of molecular biology

authors： Lipfert J,Das R,Chu VB,Kudaravalli M,Boyd N,Herschlag D,Doniach S

更新日期：2007-02-02 00:00:00