Abstract：
:Rvb1 and Rvb2 are essential AAA(+) (ATPases associated with diverse cellular activities) helicases, which are important components of critical complexes such as chromatin remodeling and telomerase complexes. The oligomeric state of the Rvb proteins has been controversial. Independent studies from several groups have described the yeast and human Rvb1/Rvb2 complex both as a single and as a double hexameric ring complex. We found that histidine-tagged constructs of yeast Rvb proteins employed in some of these studies induced the assembly of double hexameric ring Rvb1/Rvb2 complexes. Instead, untagged versions of these proteins assemble into single hexameric rings. Furthermore, purified endogenous untagged Rvb1/Rvb2 complexes from Saccharomyces cerevisiae were also found as single hexameric rings, similar to the complexes assembled in vitro from the purified untagged components. These results demonstrate that some of the differences between the reported structures are caused by histidine tags and imply that further studies on the purified proteins should be carried out using untagged constructs.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Cheung KL,Huen J,Kakihara Y,Houry WA,Ortega Jdoi
10.1016/j.jmb.2010.10.003subject
Has Abstractpub_date
2010-12-03 00:00:00pages
478-92issue
3eissn
0022-2836issn
1089-8638pii
S0022-2836(10)01089-2journal_volume
404pub_type
杂志文章abstract：:The sigma 70 (sigma(70)) subunit of Escherichia coli RNA polymerase specifies transcription from promoters that are responsible for basal gene expression during vegetative growth. When sigma(70) is present within polymerase holoenzyme, two of its domains, 2.4 and 4.2, interact with sequences within the -10 and -35 reg...
journal_title：Journal of molecular biology
pub_type： 杂志文章
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abstract：:Dystrophin Dp71 is the main product of the Duchenne muscular dystrophy gene in the brain; however, its function is unknown. To study the role of Dp71 in neuronal cells, we previously generated by antisense treatment PC12 neuronal cell clones with decreased Dp71 expression (antisense-Dp71 cells). PC12 cells express two...
journal_title：Journal of molecular biology
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journal_title：Journal of molecular biology
pub_type： 杂志文章,评审
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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journal_title：Journal of molecular biology
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doi：10.1006/jmbi.1997.1060
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journal_title：Journal of molecular biology
pub_type： 杂志文章,评审
doi：10.1016/j.jmb.2018.07.013
更新日期：2018-09-14 00:00:00
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pub_type： 杂志文章
doi：10.1006/jmbi.1998.2021
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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doi：10.1006/jmbi.1994.1430
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journal_title：Journal of molecular biology
pub_type： 杂志文章,评审
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1016/j.jmb.2004.01.024
更新日期：2004-03-12 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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更新日期：2009-08-28 00:00:00
