Abstract:
OBJECTIVES:The development of a micro plate assay for cytotoxicity testing of dental materials based on a bioassay using brine shrimp larvae (artemia salina) as sensitive organisms. METHODS:Brine shrimp larvae are commonly used for cytotoxicity assays in pharmacology. These larvae are sensitive to toxic substances. The ratio between dead larvae (no motility) and living larvae (high motility) in comparison to a control without any toxic substances is used to estimate the toxicity of the test solutions. The test materials (Arabesk((R)), Solitaire((R)), Pertac((R)) II, Tetric((R)), Herculite((R)) and the compomer materials Dyract((R)), Hytac((R)), Compoglass((R))) were polymerized and consecutively milled. After incubation of 1g in 4ml distilled water at 37 degrees C for 48h, the solid materials were separated by centrifugation. The solutions were equibrilated with NaCl to a salt content of 25g/l. Aliquots of 200microl were distributed in eight micro wells and 50microl of a artemia salina containing (n=8-14) solution were added to each well. As controls eight wells with 250microl salt solution containing a comparable number of brine shrimp were used. At baseline, after 2, 5, 24 and 48h, the dead shrimp were counted using a stereo microscope. Finally all shrimps were sacrificed using Na-acid (5%) and counted to get the number of shrimps per well. RESULTS:All compomers and Solitaire caused 100% brine shrimp lethality after 24h and showed significantly (p<0.01, signed rank test) higher toxicities than the remaining composites. With the exception of Pertac II, all composites showed significantly higher toxic values than the control. Pertac II did not show any differences from the controls used. CONCLUSIONS:This new technique has some advantages for toxicity testing of restorative materials, because it can quickly be carried out at low costs. The disadvantage is the high quantity of material used and the low sensitivity.
journal_name
J Dentjournal_title
Journal of dentistryauthors
Pelka M,Danzl C,Distler W,Petschelt Adoi
10.1016/s0300-5712(00)00007-5keywords:
subject
Has Abstractpub_date
2000-07-01 00:00:00pages
341-5issue
5eissn
0300-5712issn
1879-176Xpii
S0300-5712(00)00007-5journal_volume
28pub_type
杂志文章abstract:OBJECTIVE:To evaluate the effect of compounds of Galla chinensis on the remineralisation of initial enamel carious lesions in vitro. METHODS:Sixty bovine enamel blocks with early lesions were prepared and randomly divided into six treatment groups. The lesions were subjected to a pH-cycling regime for 12 days. Each da...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2006.11.007
更新日期:2007-05-01 00:00:00
abstract:OBJECTIVES:To investigate the current prevalence of open access (OA) in the field of dentistry, the means used to provide OA, as well as the association between OA and citation counts. METHODS:PubMed was searched for dental articles published in 2013. The OA status of each article was determined by manually checking G...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2016.02.005
更新日期:2016-04-01 00:00:00
abstract:OBJECTIVES:To evaluate the toothbrush wear resistance of infiltrated artificial white spot lesions following two infiltration strategies, and to assess their ultramorphology. METHODS:Flat enamel surfaces from freshly extracted bovine teeth were polished and immersed in a Buskes demineralising solution for 30 days to c...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2011.02.009
更新日期:2011-05-01 00:00:00
abstract:OBJECTIVES:The aim of the study was to investigate the effect of silver diamine fluoride (SDF) therapy on the oral health-related quality of life (OHRQoL) of preschool children and their families in a school-based setting. METHODS:A prospective study was conducted in six kindergartens in Hong Kong. Parents of the chil...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2018.12.004
更新日期:2019-02-01 00:00:00
abstract:OBJECTIVE:To evaluate a novel methodology using industrial scanners as a reference, and assess in vivo accuracy of 3 intraoral scanners (IOS) and conventional impressions. Further, to evaluate IOS precision in vivo. METHODS:Four reference-bodies were bonded to the buccal surfaces of upper premolars and incisors in fiv...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2017.12.006
更新日期:2018-02-01 00:00:00
abstract:OBJECTIVES:We recently developed a new rechargeable composite with nanoparticles of amorphous calcium phosphate (NACP) having long-term calcium (Ca) and phosphate (P) ion release; however, this composite was not antibacterial. The objectives of this study were to: (1) incorporate dimethylaminohexadecyl methacrylate (DM...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2016.06.003
更新日期:2016-09-01 00:00:00
abstract:OBJECTIVE:Sodium hexametaphosphate (SHMP) is a widely used industrial preservative commonly found in children's drinks. In this paper we examined the effect of SHMP incorporated into children's drinks on acid production by the oral biofilm by monitoring salivary concentrations of lactic acid. MATERIALS AND METHOD:Twel...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2006.08.003
更新日期:2007-03-01 00:00:00
abstract:OBJECTIVES:The objective of the study was to determine the perceptibility and acceptability thresholds for dental ceramics using CIEDE2000 (ΔE(00)) and CIELAB (ΔE(ab)(*)) color difference formulas and a novel TSK Fuzzy Approximation. METHODS:A 13-observer panel performed independent observations of perceptibility and ...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2010.07.008
更新日期:2010-01-01 00:00:00
abstract:OBJECTIVE:This study aimed to evaluate the addition of copper nanoparticles (CuNp) on the clinical performance of a universal adhesive system used as etch-and-rinse (ER) and self-etch (SE). METHODS:216 restorations were randomly placed in 36 subjects according to the following groups: ERcu = etch-and-rinse with 0.1% C...
journal_title:Journal of dentistry
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.jdent.2019.103219
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVE:The study evaluated the clinical performance and marginal adaptation of direct and semi-direct class II composite restorations in a split-mouth design over 3.5 years. DESIGN:44 upper posterior teeth in 11 adults with primary carious lesions were treated with 22 direct and 22 semi-direct restorations. Convent...
journal_title:Journal of dentistry
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/j.jdent.2004.11.009
更新日期:2005-07-01 00:00:00
abstract:OBJECTIVES:This in vitro study examined the resistance to displacement offered by guide planes and the displacement mechanics of a bilateral bounded saddle cast cobalt chrome alloy removable partial denture framework under conditions simulating an average initial fit. METHODS:A specially designed model mouth jig fitte...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/s0300-5712(01)00016-1
更新日期:2001-05-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to compare inhibition, in vitro, of a resin-modified and a conventional glass ionomer cement. METHODS:Standardized Class V cavities were prepared in extracted human molar teeth and restored with Fuji II LC (n = 14) or Chemfil II (n = 14) according to manufacturers' guidelines. The ...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/0300-5712(95)00051-8
更新日期:1996-01-01 00:00:00
abstract:OBJECTIVES:To investigate the effects of acidic and neutral 30% hydrogen peroxide (HP) on human tooth enamel in terms of chemical structure, mechanical property, surface morphology and tooth colour. METHODS:Twenty-seven human dental blocks were obtained from premolars and randomly divided into three groups (n=9): Grou...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2011.07.011
更新日期:2011-10-01 00:00:00
abstract:OBJECTIVES:To evaluate the effect of addition of zinc oxide and copper nanoparticles (ZnO/CuNp) into universal adhesives, on antimicrobial activity (AMA), cytotoxicity (CTX), water sorption (WS) and solubility (SO), microhardness (MH) and in vitro degree of conversion (DC), as well as resin-dentin microtensile bond str...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2019.01.012
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVES:Understanding the mechanical properties of dentine is of importance as adhesive restorative materials mainly achieve their bonding to the tooth structure through dentine. The current study measures the hardness and modulus of elasticity of primary molar dentine using an Ultra-Micro-Indentation System (UMIS),...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/s0300-5712(03)00045-9
更新日期:2003-05-01 00:00:00
abstract:OBJECTIVES:The aim of this in vitro study was to evaluate the penetration of an infiltrant and a sealant, when applied as recommended, into fissure caries lesions. METHODS:The fissure systems of extracted human teeth were classified according to the international caries detection and assessment system (ICDAS, codes: 0...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2014.01.006
更新日期:2014-04-01 00:00:00
abstract::Replacement of pin-retained amalgam restorations is complicated by the loss of prime pin sites and remaining pin sites posing anatomical problems. Self-threading dentine pins can be removed with an ultrasonic handpiece, and the pin site reused with a large pin. Retention of replacement pins is similar to original pins...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/0300-5712(93)90109-4
更新日期:1993-10-01 00:00:00
abstract:OBJECTIVES:Methacrylic compounds such as 2-hydroxyethyl methacrylate (HEMA), triethylene glycol dimethacrylate (TEGDMA) and bisphenol A glycerolate (1 glycerol/phenol) dimethacrylate (Bis-GMA) are largely present in auto- or photopolymerizable composite resins. Since the polymerization reaction is never complete, these...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2006.07.008
更新日期:2007-03-01 00:00:00
abstract::Tooth wear attributed to the physical wear of teeth by clenching and grinding (attrition) has been described previously in alcoholic patients. However, the pattern of wear seen in the series of cases reported here is more consistent with chemical damage (erosion) than attrition. A possible mechanism for this process i...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/0300-5712(89)90168-1
更新日期:1989-10-01 00:00:00
abstract:OBJECTIVES:A monomer methacryloyloxydodecylpyridinium bromide (MDPB) has antibacterial activity before polymerization. Furthermore, the antibacterial agent is immobilized by the polymerization of MDPB and the resin-based material incorporating MDPB is able to show an antibacterial effect even after being cured. The pur...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/s0300-5712(97)00013-4
更新日期:1998-03-01 00:00:00
abstract:OBJECTIVE:The purpose of this study was to clarify the positional relationship between the crown contour and the pulp chamber using micro-CT system for restorations of maxillary second deciduous molar. METHODS:Five maxillary second deciduous molar teeth each from the deciduous dentition and the mixed dentition periods...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2005.12.001
更新日期:2006-08-01 00:00:00
abstract:OBJECTIVES:The aim of this in vivo study was to evaluate the effects of a home-bleaching agent on the color and surface properties of four tooth-colored restorative materials. METHODS:Two composite resins (a nano-hybrid and a packable), a polyacid-modified composite and a conventional glass-ionomer were tested. Specim...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2009.01.003
更新日期:2009-05-01 00:00:00
abstract:OBJECTIVES:The bonding of acrylic resin to dental prostheses constructed from metal has been the subject of extensive research in recent years, much of it between base-metal alloys such as Ni-Cr alloys and dental composite. The development of techniques for the manufacture of dental appliances from titanium and titaniu...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/s0300-5712(96)00051-6
更新日期:1997-09-01 00:00:00
abstract:OBJECTIVES:Manufacturers are adding fluoride (F) to calcium-containing chewing gums to further promote enamel remineralization. The aim of this study was to assess the effect of a chewing gum containing phosphoryl oligosaccharides of calcium (POs-Ca) and fluoride on remineralization of enamel subsurface lesions, in a d...
journal_title:Journal of dentistry
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.jdent.2011.08.009
更新日期:2011-11-01 00:00:00
abstract:OBJECTIVES:The light intensity of curing lights used in private dental offices was measured using commercial curing and heat radiometers and related to uniformity of cure depth of standardized composite specimens. METHODS:The intensity of 130 curing light from 107 dental offices was measured with curing and heat radio...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/s0300-5712(98)00052-9
更新日期:1999-03-01 00:00:00
abstract:OBJECTIVES:This study was performed to determine the compositions of commercial temporary restorative resins and to evaluate the leachability of plasticizer and residual monomer from them. METHODS:The chemicals in four commercial temporary restorative resins (Dura Seal, Fit Seal, Plast Seal Quick, and Poly Seal) were ...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2003.12.004
更新日期:2004-05-01 00:00:00
abstract:OBJECTIVE:An accurate evaluation of the extent of dental caries and possible progress of the lesion from enamel into dentin is very important in clinical dentistry. Dentists need an imaging technology that can noninvasively and reliably quantify the extent of caries. This study aimed to evaluate the effectiveness of no...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2012.10.007
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVES:Previously, we have reported that dental composite incorporating the new monomer methacryloyloxydodecylpyridinium bromide (MDB) showed no release of antibacterial components after being cured but still exhibited antibacterial activity against Streptococcus mutants on its surface. In this study, in order to e...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/0300-5712(95)93576-n
更新日期:1995-06-01 00:00:00
abstract:OBJECTIVES:The purpose of the study was to evaluate the intra- and inter-examiner variability of a panel of examiners using a 'glance and grade' marking system when assessing Class II preparations. METHODS:The examination panel consisted of five university staff with varying clinical and teaching experience. The panel...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/s0300-5712(97)00033-x
更新日期:1998-11-01 00:00:00
abstract:OBJECTIVES:To integrate items from two widely used oral health-related quality of life (OHRQoL) questionnaires, the General Oral Health Assessment Index (GOHAI) and the Oral Impacts on Daily Performances (OIDP), as well as culturally-specific items of the Oral Health Impact Profile (OHIP) into a four-dimensional OHRQoL...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2016.06.006
更新日期:2016-10-01 00:00:00