Abstract:
:Fluorescence in situ hybridization (FISH) was used to localize two transgenes (gus and bar), carried on plasmids pACT-1F and pUBA, respectively, on mitotic metaphase squashes of T1 plants of the cultivated hexaploid oat Avena sativa L. cotransformed by microprojectile bombardment of embryogenic callus. Among the eight progeny analysed by FISH in each of two lines, we detected plants null, hetero- and homozygous for the two genes in one line, and plants null and heterozygous for the two genes in the other line. Our results demonstrated that in the two independent transformation events, the gus and bar genes had inserted in the same position relative to each other. In each transformation event, the insertions occurred on D satellite (SAT) chromosomes bearing a C genome translocation.
journal_name
Heredity (Edinb)journal_title
Heredityauthors
Leggett JM,Perret SJ,Harper J,Morris Pdoi
10.1046/j.1365-2540.2000.00627.xkeywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
46-53eissn
0018-067Xissn
1365-2540pii
her627journal_volume
84 ( Pt 1)pub_type
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