Chromosomal localization of cotransformed transgenes in the hexaploid cultivated oat Avena sativa L. using fluorescence in situ hybridization.

Abstract:

:Fluorescence in situ hybridization (FISH) was used to localize two transgenes (gus and bar), carried on plasmids pACT-1F and pUBA, respectively, on mitotic metaphase squashes of T1 plants of the cultivated hexaploid oat Avena sativa L. cotransformed by microprojectile bombardment of embryogenic callus. Among the eight progeny analysed by FISH in each of two lines, we detected plants null, hetero- and homozygous for the two genes in one line, and plants null and heterozygous for the two genes in the other line. Our results demonstrated that in the two independent transformation events, the gus and bar genes had inserted in the same position relative to each other. In each transformation event, the insertions occurred on D satellite (SAT) chromosomes bearing a C genome translocation.

journal_name

Heredity (Edinb)

journal_title

Heredity

authors

Leggett JM,Perret SJ,Harper J,Morris P

doi

10.1046/j.1365-2540.2000.00627.x

keywords:

subject

Has Abstract

pub_date

2000-01-01 00:00:00

pages

46-53

eissn

0018-067X

issn

1365-2540

pii

her627

journal_volume

84 ( Pt 1)

pub_type

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