Abstract:
:Mannose-labeled glycopeptides from the surface of growing and nongrowing human diploid cells (KL-2) were separated into neutral glycopeptides and acidic glycopeptides by paper electrophoresis. Growth-dependent alterations occurred in oligomannosyl cores in the neutral glycopeptides; namely, the neutral glycopeptides from the surface of growing cells were more resistant to endo-beta-N-acetylglucosaminidase D but were more susceptible to alpha-mannosidase (EC 3.2.1.24; alpha-D-mannose mannohydrolase) than those derived from the surface of nongrowing cells. Another growth-dependent change was found when the endoglycosidase-resistant material from acidic glycopeptides was compared by paper electrophoresis at pH 1.9. The material from the surface of nongrowing cells contained a component that was absent or greatly reduced in growing cells.
journal_name
Proc Natl Acad Sci U S Aauthors
Ceccarini C,Muramatsu T,Tsang J,Atkinson PHdoi
10.1073/pnas.72.8.3139keywords:
subject
Has Abstractpub_date
1975-08-01 00:00:00pages
3139-43issue
8eissn
0027-8424issn
1091-6490journal_volume
72pub_type
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