Abstract:
OBJECTIVE:Calcium-dependent neutral cysteine proteinase (calpain) was originally referred to as an intracellular enzyme. However, recently it has come to be considered as an extracellular matrix proteinase, as well having a degrading effect on cartilage proteoglycan. In the present study we sought to determine whether human articular cartilage chondrocytes themselves have the capability to produce and secrete this interesting proteinase. METHODS:Human articular cartilage tissue cultures from osteoarthritic (11 specimens from 7 patients) and rheumatoid arthritic (3 specimens from 2 patients) knee joints were established, and the m-calpain released into the culture medium was concentrated and detected by immunoelectrophoretic blotting. The presence of m-calpain in the arthritic cartilage was also examined by immunohistochemistry before and after culturing. RESULTS:M-calpain was detectable in all of the cartilage tissue culture supernatants (conditioned medium) by western blotting. Positive intracellular immunostaining of m-calpain in chondrocytes was observed in all samples. Furthermore, m-calpain was found to be present in the matrix and on the articular surface of the cartilage in half of the specimens. CONCLUSION:The findings of our experiment suggest that cartilage chondrocytes may actively take part in m-calpain production and that they may have the capacity to release it into the extracellular matrices.
journal_name
Clin Exp Rheumatoljournal_title
Clinical and experimental rheumatologyauthors
Szomor Z,Shimizu K,Yamamoto S,Yasuda T,Ishikawa H,Nakamura Tkeywords:
subject
Has Abstractpub_date
1999-09-01 00:00:00pages
569-74issue
5eissn
0392-856Xissn
1593-098Xjournal_volume
17pub_type
杂志文章abstract::Patients with ankylosing spondylitis (AS) may develop cardiovascular manifestations ranging from asymptomatic forms to life threatening conditions. The most important cardiovascular manifestation of AS is aortitis, which frequently involves the aortic root and the ascending aorta leading to valvular insufficiency. The...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,评审
doi:
更新日期:2008-05-01 00:00:00
abstract::Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) effects in Ankylosing Spondylitis (AS) are only suspensive but because of their rapid efficacy on inflammatory symptoms they are the first-line treatment in AS. Short term efficacy of NSAIDs in AS is observed for most patients but the correlation of NSAID intake with the l...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2002-11-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:1989-11-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:1998-03-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2012-05-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:1985-07-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2011-03-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2006-09-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2011-11-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2015-05-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2020-07-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,评审
doi:
更新日期:2017-09-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1997-05-01 00:00:00
abstract:OBJECTIVES:To evaluate the micro-RNA (miRNA) expression profile in patients with early psoriatic arthritis (PsA) in order to assess the role of miRNAs as potential PsA biomarkers. METHODS:The expression of 723 mature miRNAs in peripheral blood mononuclear cells of early PsA patients in comparison with early-rheumatoid...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2017-01-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2014-05-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,meta分析,评审
doi:
更新日期:2013-09-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,实务指引,评审
doi:
更新日期:2016-09-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,评审
doi:
更新日期:2020-01-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,评审
doi:
更新日期:2019-09-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,评审
doi:
更新日期:2017-03-01 00:00:00
abstract:OBJECTIVES:Ankylosing spondylitis (AS) is a chronic, progressive, and disabling disease, but the diagnosis is often missed and markedly delayed. An early diagnosis is important to establish a treatment to reduce disability and modify the natural course of disease. The aim of this study was to investigate the diagnostic...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2012-07-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2011-11-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2008-09-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2008-07-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2020-09-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2020-09-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,评审
doi:
更新日期:2007-01-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,评审
doi:
更新日期:2015-07-01 00:00:00