当前位置： SCI文献检索 > MYCOPATHOLOGIA期刊下所有文献 > The effects of four mycotoxins on the mitogen stimulated proliferation of bovine peripheral blood mononuclear cells in vitro.

The effects of four mycotoxins on the mitogen stimulated proliferation of bovine peripheral blood mononuclear cells in vitro.

Abstract：

:The effects of four mycotoxins, T-2 toxin, deoxynivalenol (DON), ochratoxin A (OTA) and fumonisin B1 (FB1) on the response of bovine peripheral blood mononuclear cells (PBM) in vitro to the mitogens concanavalin A (Con A), phytohaemagglutinin A (PHA) and pokeweed mitogen (PWM) were assayed after 4 days' incubation using 3H-thymidine uptake and the MTT bioassay. The concentrations of mycotoxin required to reduce the proliferative response of PBM by 50% for Con A, PHA and PWM as measured by 3H-thymidine incorporation was for T-2 toxin 0.30, 0.40 and 0.18 ng ml-1; for DON 0.07, 0.09 and 0.04 microgram ml-1; for OTA 0.10, 0.20 and 0.15 microgram ml-1, and for FB1 35, 18 and 11 micrograms ml-1 respectively. The concentrations of mycotoxin required to reduce the proliferative response of PBM by 50% for Con A, PHA and PWM as measured by the MTT bioassay were for T-2 toxin 2.0, 2.0 and 1.0 mg ml-1; for DON 0.70, 0.50 and 0.50 microgram ml-1; OTA 1.5, 1.5 and 1.5 micrograms ml-1; and FB1 > 50, > 50 and 20 micrograms ml-1 respectively. Further cytotoxicity assays including the LDH bioassay and Trypan blue exclusion were performed only on Con A-stimulated PBM cells after 72 h incubation. With the LDH-bioassay the 50% inhibition levels were T-2 toxin 0.3 ng ml-1, DON 0.4 microgram ml-1, OTA 1.4 micrograms ml-1 and FB1 3.5 micrograms ml-1; for Trypan blue uptake the 50% inhibition levels were T-2 toxin 5 ng ml-1, DON 2.3 micrograms ml-1 and OTA 4 micrograms ml-1 respectively.

journal_name

Mycopathologia

journal_title

Mycopathologia

authors

Charoenpornsook K,Fitzpatrick JL,Smith JE

doi

10.1023/a:1006971724678

keywords:

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

105-11

issue

2

eissn

0301-486X

issn

1573-0832

journal_volume

143

pub_type

杂志文章

相关文献

MYCOPATHOLOGIA文献大全
  • Cytotoxicity of mycotoxins evaluated by the MTT-cell culture assay.

    abstract：:The application of a modified colorimetric bioassay for the evaluation of the biological effects of mycotoxins is reported. Using three different monolayer cell lines (swine kidney, Madin Darby canine kidney, HeLa) the influence of nine different mycotoxins on the cellular methylthiazoltetrazolium (MTT)-cleavage activ...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/BF01138479

    authors： Hanelt M,Gareis M,Kollarczik B

    更新日期：1994-12-01 00:00:00

  • Effect of Citrus reticulata and Cymbopogon citratus essential oils on Aspergillus flavus growth and aflatoxin production on Asparagus racemosus.

    abstract：:Essential oils extracted from Citrus reticulata and Cymbopogon citratus were tested in vitro against the toxigenic strain of Aspergillus flavus, isolated from the tuberous roots of Asparagus racemosus, used in preparation of herbal drugs. The essential oils completely inhibited the growth of A. flavus at 750 ppm and a...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/s11046-010-9311-8

    authors： Singh P,Shukla R,Kumar A,Prakash B,Singh S,Dubey NK

    更新日期：2010-09-01 00:00:00

  • Tinea corporis due to Trichophyton rubrum in a woman and Tinea capitis in her 15-day-old baby: molecular evidence of vertical transmission.

    abstract：:We report a case of a 40-year-old Caucasian woman who came under our observation with a 7-year history of a chronic erythematous scaly dermatitis, diagnosed as psoriasis, involving gluteal area and thighs, and treated with topical steroids without benefit. During pregnancy, a progressive worsening of her condition and...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/s11046-011-9492-9

    authors： Mapelli ET,Borghi E,Cerri A,Sciota R,Morace G,Menni S

    更新日期：2012-03-01 00:00:00

  • Coccidioidomycosis in Patients with Selected Solid Organ Cancers: A Case Series and Review of Medical Literature.

    abstract：:Coccidioidomycosis is a common infection in the desert southwestern USA; approximately 3 % of healthy persons in Arizona alone become infected annually. Coccidioidomycosis may be severe in immunocompromised persons, but experience among patients with solid organ cancer has not been fully described. Therefore, we aimed...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/s11046-016-0044-1

    authors： Fitterer C,Berg Z,Halfdanarson TR,Vikram HR,Kusne S,Orenstein R,Seville MT,Blair JE

    更新日期：2016-12-01 00:00:00

  • Resistance Mechanism in a Terbinafine-Resistant Strain of Microsporum canis.

    abstract：:To clarify the terbinafine (TRF) resistance mechanism in a TRF-resistant strain of Microsporum canis, the expression of the pleiotropic drug resistance (PDR1), multidrug resistance (MDR1), MDR2 and MDR4 genes were investigated by real-time quantitative PCR (RT-qPCR) analysis, given the known interaction of the corresp...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/s11046-018-0242-0

    authors： Kano R,Hsiao YH,Han HS,Chen C,Hasegawa A,Kamata H

    更新日期：2018-06-01 00:00:00

  • Isolation of scopoletin from leaves of Hevea brasiliensis and the effect of scopoletin on pathogens of H. brasiliensis.

    abstract：:Scopoletin (7-hydroxy-6-methoxy coumarin) which inhibited the conidial germination of Corynespora cassiicola was isolated from the uninfected mature leaves of Hevea brasiliensis. Scopoletin was not detected in uninfected immature rubber leaves. The immature leaves produced scopoletin after being infected with C. cassi...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1023/a:1014910132595

    authors： Silva WP,Deraniyagala SA,Wijesundera RL,Karunanayake EH,Priyanka UM

    更新日期：2002-01-01 00:00:00

  • Production of a fungistatic substance by Pseudallescheria boydii isolated from soil amended with vegetable tissues and its significance.

    abstract：:Four fungal isolates that were able to use vegetable tissues for multiplication in soil were isolated and identified as Pseudallescheria boydii based on morphological characteristics and ITS sequence similarity. When grown in broth prepared from the same vegetable tissues used in soil amendment, all these isolates of ...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/s11046-009-9237-1

    authors： Ko WH,Tsou YJ,Ju YM,Hsieh HM,Ann PJ

    更新日期：2010-02-01 00:00:00

  • Incidence of histoplasmin skin test reactivity in Somalia: an epidemiological study.

    abstract：:Histoplasmosis is an important systemic mycotic infection with a wide geographic distribution. Its occurrence has been mostly studied in the US (6) and in Central America (6), but very little is known about its distribution in Africa, where a specific variant exists. Skin test surveys in the Democratic Republic of Som...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/BF00437292

    authors： Maresca B,Ali AI,Kobayashi GS,Sacco M

    更新日期：1987-05-01 00:00:00

  • Identification of Pythium carolinianum causing 'root rot' of cotton in Egypt and its possible biological control by Pseudomonas fluorescens.

    abstract：:A severe root rot disease of cotton caused by Pythium carolinianum was diagnosed in a cotton field in Beni-Musa village, 20 km southwest of El-Minia city, Egypt, during the summer of 1996. This was the first reported isolation of this fungus in Egypt. In the light of the importance of the cotton industry in Egypt, res...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1023/A:1006906212689

    authors： Abdelzaher HM,Elnaghy MA

    更新日期：1998-01-01 00:00:00

  • Red grain mycetoma of the scalp (Actinomadura pelletieri). A case report from India.

    abstract：:A case of red grain mycetoma on the scalp is described. The disease, caused by Actinomadura pelletieri, is extremely rare in this part of the world, and from India this is perhaps the first case with scalp involvement. The lesion showed satisfactory response to partial excision followed by chemotherapy with streptomyc...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/BF00441259

    authors： Bedi TR,Kaur S,Kumar B

    更新日期：1978-07-28 00:00:00

  • Retrospective analysis of 15 cases of Penicilliosis marneffei in a southern China hospital.

    abstract：:We retrospectively analyzed the medical records and clinical characteristics of 15 patients diagnosed with Penicilliosis marneffei (PSM) between January 1, 1993, and December 31, 2012, at the Third Affiliated Hospital of Sun Yat-sen University. The most common symptoms of PSM were fever (14/15, 93 %), cough (13/15, 87...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/s11046-014-9737-5

    authors： Zhou F,Bi X,Zou X,Xu Z,Zhang T

    更新日期：2014-06-01 00:00:00

  • Identification of a Candida parapsilosis strain producing extracellular serine peptidase with keratinolytic activity.

    abstract：:A yeast strain isolated from feather waste from a chicken processing plant was identified as Candida parapsilosis by biochemical tests and morphological studies. The yeast was able to grow in phosphate-buffered saline supplemented with 1% native feather as the sole carbon and nitrogen source. A keratin substrate was o...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/s11046-009-9231-7

    authors： Vermelho AB,Mazotto AM,de Melo AC,Vieira FH,Duarte TR,Macrae A,Nishikawa MM,da Silva Bon EP

    更新日期：2010-01-01 00:00:00

  • Fungal flora of Egyptian baladi bread with special reference to the mutagenic effects of their toxic metabolites.

    abstract：:The fungal flora of wheat flour and baladi bread in upper Egypt were investigated. Most of the isolated fungal species belong to the genus Aspergillus. The presence of non heat resistant fungi of the both flat surfaces of baladi bread, came from contamination after baking and from improper handling at homes. Among the...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/BF00437130

    authors： Megalla SE,Abdou RF,Bagy MM

    更新日期：1985-01-01 00:00:00

  • Mycological analysis of the sands of a box for children's play.

    abstract：:Ecologic and hygienic interests led us to analyze the mycoflora of the sands of a sand box for children's play in Turin. Along with traditional methods, we have also employed selective techniques suitable for the isolation of cellulolytic and keratinolytic fungi. A total of 89 species were isolated. As supported by th...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/BF00437178

    authors： Filipello Marchisio V,Luppi Mosca AM

    更新日期：1982-10-22 00:00:00

  • In vitro activities of caspofungin, amphotericin B and azoles against Coccidioides posadasii strains from Northeast, Brazil.

    abstract：:Coccidioidomycosis is a systemic infection caused by the soil-dwelling dimorphic fungi Coccidioides spp. The disease is endemic in semiarid Northeast Brazil, where it is caused by C. posadasii. The aim of this study was to perform antifungal susceptibility tests of clinical and environmental strains of C. posadasii fr...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/s11046-005-0177-0

    authors： Cordeiro RA,Brilhante RS,Rocha MF,Fechine MA,Costa AK,Camargo ZP,Sidrim JJ

    更新日期：2006-01-01 00:00:00

  • Hair and scalp mycobiota in school children in Nablus area.

    abstract：:Hair and scalp mycobiota of 1389 clinically normal children aged 6-12 years attending 12 schools in the Nablus District, Palestinian Authority, was assessed on three occasions over 8-month period (October 1998-May 1999) using the hair brush technique. One hundred and one fungal species belonging to 33 genera were reco...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1023/a:1010989431375

    authors： Ali-Shtayeh MS,Salameh AA,Abu-Ghdeib SI,Jamous RM

    更新日期：2001-01-01 00:00:00

  • Genes Encoding Proteolytic Enzymes Fungalysin and Subtilisin in Dermatophytes of Human and Animal Origin: A Comparative Study.

    abstract：:Dermatophytes are among the most successful fungal pathogens in humans, but their virulence mechanisms have not yet been fully characterized. Dermatophytic fungi secrete proteases in vivo, which are responsible for fungal colonization and degradation of the keratinized tissue during infection. In the present study, we...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/s11046-019-00367-2

    authors： Kaplan E,Gonca S,Kandemir H,Döğen A,Hilmioğlu-Polat S,Ilkit M,Tanaka R,Yaguchi T,Uhrlaβ S,Nenoff P

    更新日期：2020-02-01 00:00:00

  • Further toxic properties of the fungal metabolite dothistromin.

    abstract：:The toxicity of the fungal phytotoxin dothistromin (l) to microorganisms, its lysis of human red blood corpuscles and beetroot tissue, and its unexpectedly selective inhibition of radicle elongation for Trigonella foenum-graecum were strongly light-dependent. Dothistromin was also toxic to Artemia salina but without r...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/BF00436652

    authors： Stoessl A,Abramowski Z,Lester HH,Rock GL,Towers GH

    更新日期：1990-12-01 00:00:00

  • Reappraisal of Conventional Diagnosis for Dermatophytes.

    abstract：:Dermatophytoses include a wide variety of diseases involving glabrous skin, nails and hair. These superficial infections are a common cause of consultation in dermatology. In many cases, their diagnosis is not clinically obvious, and mycological analysis therefore is required. Direct microscopic examination of the sam...

    journal_title：Mycopathologia

    pub_type： 杂志文章,评审

    doi：10.1007/s11046-016-0071-y

    authors： Pihet M,Le Govic Y

    更新日期：2017-02-01 00:00:00

  • Optimal concentration of ammonium ion in a minimal synthetic medium for the growth of Candida albicans.

    abstract：:Candida albicans strain B 311-10 with and without starvation was cultivated in the minimal synthetic medium of Shepherd et al., modified without biotin, amino acids, low glucose concentration and with decreasing amounts of (NH4)2SO4, to determine the optimal growth requirement for this strain. All the experiments were...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/BF00436116

    authors： Vidotto V,Ochoa LG,Cortes JM,Bruatto M

    更新日期：1991-03-01 00:00:00

  • In vitro Candida albicans biofilm induced proteinase activity and SAP8 expression correlates with in vivo denture stomatitis severity.

    abstract：:Denture stomatitis is a common inflammatory disorder of the palatal mucosa amongst denture wearers. The pathological changes are induced by Candida albicans biofilm on the fitting surface of the upper denture, and different individuals experience different levels of disease. C. albicans is known to produce secreted as...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/s11046-012-9522-2

    authors： Ramage G,Coco B,Sherry L,Bagg J,Lappin DF

    更新日期：2012-07-01 00:00:00

  • Cytologic findings in 43 cases of blastomycosis diagnosed ante-mortem in naturally-infected dogs.

    abstract：:A retrospective study of the cytology of blastomycosis was undertaken. Sixty-one samples from 43 naturally infected dogs diagnosed ante-mortem by means of cytology were reviewed. Skin and lymph nodes rendered the highest number of positive samples with 17 out of 18 and 14 out of 17 respectively. Transtracheal washes c...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/BF01102884

    authors： Garma-Aviña A

    更新日期：1995-08-01 00:00:00

  • Fumonisin production by Fusarium species isolated from freshly harvested corn in Iran.

    abstract：:Fifty-one strains of Fusarium verticillioides and F. proliferatum isolated from corn collected from four different geographic areas in Iran, namely Fars, Khuzestan, Kermanshah and Mazandaran (an endemic oesophageal cancer (OC) area) were evaluated for their ability to produce fumonisins B1 (FB1), B2 (FB2) and B3 (FB3)...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/s11046-004-3899-5

    authors： Ghiasian SA,Rezayat SM,Kord-Bacheh P,Maghsood AH,Yazdanpanah H,Shephard GS,van der Westhuizen L,Vismer HF,Marasas WF

    更新日期：2005-01-01 00:00:00

  • Fine structural studies of the Chrysosporium state of Renispora flavissima.

    abstract：:Aspects of the fine structural characteristics of conidial and hyphal cells of the imperfect Chrysosporium state of the newly reported gymnoascaceous fungus, Renispora flavissima, are described and illustrated by electron micrographs of ultrathin sections. Preservation of the substructure of the thick-walled, smooth t...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/BF00443903

    authors： Garrison RG,Mirikitani FK

    更新日期：1983-01-17 00:00:00

  • Effects of antifungal agents alone and in combination against Candida glabrata strains susceptible or resistant to fluconazole.

    abstract：:The rise of Candida spp. resistant to classic triazole antifungal agents has led to a search for new therapeutic options. Here, we evaluated combinations of antifungals in a checkerboard assay against two groups of Candida glabrata strains: one containing fluconazole-susceptible clinical isolates (FS) and another cont...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/s11046-012-9538-7

    authors： Alves IA,Bandeira LA,Mario DA,Denardi LB,Neves LV,Santurio JM,Alves SH

    更新日期：2012-09-01 00:00:00

  • Aspergillosis in German cockroach Blattella germanica (L.) (Blattoidea: Blattellidae).

    abstract：:Natural infection of Aspergillus flavus was observed in adults of Blattella germanica. Though the adult insects exhibited no external symptoms, they became hypoactive and later died. The dead and experimentally infected insects repeatedly yielded Aspergillus flavus in culture on Czapek's medium. Direct microscopic obs...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1023/a:1006859620780

    authors： Kulshrestha V,Pathak SC

    更新日期：1997-01-01 00:00:00

  • Electrophoretic migration of Paracoccidioides brasiliensis specific antigenic fraction.

    abstract：:Using the technique of immunoelectroosmophoresis-immunodiffusion (IEOP-ID), two antigenic fractions of Paracoccidioides brasiliensis one of them species-specific, produced precipitin bands in both the cathodic and anodic zones. Reactions of complete identity among the bands formed in these zones were demonstrated by a...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/BF00493814

    authors： Conti-Díaz IA,Mackinnon JE

    更新日期：1980-10-31 00:00:00

  • Cytobrush-culture method to diagnose tinea capitis.

    abstract：:This is a comparative study to isolate the dermatophytes of tinea capitis using the cytobrush and comparing it versus the standard method. A prospective, observational, comparative trial of 178 probable cases of tinea capitis was conducted in two dermatological centers. Each patient underwent mycological tests that in...

    journal_title：Mycopathologia

    pub_type： 临床试验,杂志文章

    doi：10.1007/s11046-007-9019-6

    authors： Bonifaz A,Isa-Isa R,Araiza J,Cruz C,Hernández MA,Ponce RM

    更新日期：2007-06-01 00:00:00

  • Immunohistochemical Cross-Reactivity Between Arthrographis kalrae and Highly Pathogenic Coccidioides posadasii, Histoplasma capsulatum, and Paracoccidioides Fungal Species.

    abstract：:Recently, we have reported serological cross-reactivity between paracoccidioidomycosis ceti and paracoccidioidomycosis, histoplasmosis, and coccidioidomycosis. However, data on the interaction of Arthrographis kalrae with the above pathogenic fungal infections are lacking. A. kalrae is a widely occurring ascomycetous ...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/s11046-019-00348-5

    authors： Shumoto G,Nagashima LA,Itano EN,Minakawa T,Ueda K,Sano A

    更新日期：2019-06-01 00:00:00

  • Pathogenicity of some Chrysosporium species isolated in France.

    abstract：:In order to appreciate the pathogenicity of several geophilic Chrysosporium species (including Anixiopsis stercoraria, Chrysosporium keratinophilum, C. tropicum, C. pannorum, C. state Arthroderma curreyi, C. state of A. multifidum, and C. state of A. tuberculatum), the authors have realized two series of experimental ...

    journal_title：Mycopathologia

    pub_type： 杂志文章

    doi：10.1007/BF00443059

    authors： Chabasse D,De Gentile L,Bouchara JP

    更新日期：1989-06-01 00:00:00