当前位置： SCI文献检索 > MEDICAL SCIENCE MONITOR期刊下所有文献 > Underlying Genes Involved in Atherosclerotic Macrophages: Insights from Microarray Data Mining.

Underlying Genes Involved in Atherosclerotic Macrophages: Insights from Microarray Data Mining.

Abstract：

:BACKGROUND In an atherosclerotic artery wall, monocyte-derived macrophages are the principal mediators that respond to pathogens and inflammation. The present study aimed to investigate potential genetic changes in gene expression between normal tissue-resident macrophages and atherosclerotic macrophages in the human body. MATERIAL AND METHODS The expression profile data of GSE7074 acquired from the Gene Expression Omnibus (GEO) database, which includes the transcriptome of 4 types of macrophages, was downloaded. Diﬀerentially expressed genes (DEGs) were identified using R software, then we performed functional enrichment, protein‑protein interaction (PPI) network construction, key node and module analysis, and prediction of microRNAs (miRNAs)/transcription factors (TFs) targeting genes. RESULTS After data processing, 236 DEGs were identified, including 21 upregulated genes and 215 downregulated genes. The DEG set was enriched in 22 significant Gene Ontology (GO) terms and 25 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and the PPI network constructed with these DEGs comprised 6 key nodes with degrees ≥8. Key nodes in the PPI network and simultaneously involved in the prime modules, including rhodopsin (RHO), coagulation factor V (F5), and bestrophin-1 (BEST1), are promising for the prediction of atherosclerotic plaque formation. Furthermore, in the miRNA/TF-target network, hsa-miR-3177-5p might be involved in the pathogenesis of -atherosclerosis via regulating BEST1, and the transcription factor early growth response-1 (EGR1) was found to be a potential promoter in atherogenesis. CONCLUSIONS The identified key hub genes, predicted miRNAs/TFs, and underlying molecular mechanisms may be involved in atherogenesis, thus potentially contributing to the treatment and diagnosis of patients with atherosclerotic disease.

journal_name

Med Sci Monit

journal_title

Medical science monitor : international medical journal of experimental and clinical research

authors

Wang W,Zhang K,Zhang H,Li M,Zhao Y,Wang B,Xin W,Yang W,Zhang J,Yue S,Yang X

doi

10.12659/MSM.917068

subject

Has Abstract

pub_date

2019-12-25 00:00:00

pages

9949-9962

eissn

1234-1010

issn

1643-3750

pii

917068

journal_volume

pub_type

杂志文章

在线工具

Underlying Genes Involved in Atherosclerotic Macrophages: Insights from Microarray Data Mining.