Abstract:
:Programmed cell death ligand 1 (PD-L1) is a key suppressor of the cytotoxic immune response. In colorectal carcinoma (CRC), PD-L1 expression results in immune escape and poor prognosis. Extensive researches suggested that metformin had a potential efficacy of enhancing anti-tumor immune response in different types of cancer. However, the detail mechanisms underlying the efficacy in CRC are unclear. Here, we showed that metformin decreases PD-L1 and YAP1 expression in vitro and in vivo. After silencing or inhibiting YAP1 expression by Verteporfin (VP), the inhibitor of YAP1, the expression of PD-L1 were decreased in protein level in CRC cells. Furthermore, metformin directly phosphorylated YAP1 and restricted YAP1 to entry in the nucleus, so that PD-L1 was reduced via western blot and immunofluorescence assays in SW480 and HCT116 cells. Finally, subcutaneous xenotransplanted tumor models of HCT116 cells were established in BALB/c nude mice. Compared with the control group, PD-L1 and YAP1 expressions in tumor tissues, detected by immunohistochemistry, were reduced in the group of metformin treatment. These findings illuminate a new regulatory mechanism, metformin activates Hippo signaling pathway to regulate PD-L1 expression and suggests that metformin has the possibility to increase the efficacy of immunotherapy in human CRC.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Zhang JJ,Zhang QS,Li ZQ,Zhou JW,Du Jsubject
Has Abstractpub_date
2019-11-15 00:00:00pages
6965-6976issue
11issn
1943-8141journal_volume
11pub_type
杂志文章abstract::The aim of this study was to investigate the in vivo and in vitro effects of miR-665 on sevoflurane anesthesia-induced cognitive dysfunction. SH-SY5Y cells and male SD rats were treated with sevoflurane to simulate anesthesia-induced cognitive dysfunction. The cells and rats both were transfected with a miR-665 mimic,...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-03-15 00:00:00
abstract::Gastric cancer (GC) causes nearly one million deaths worldwide each year. However, the molecular pathway of GC development remains unclear. Increasing evidences have shown that microRNAs (miRNAs) are highly associated with tumor development. However, relative little is known about the potential role of miRNAs in gastr...
journal_title:American journal of translational research
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2012-01-01 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-03-15 00:00:00
abstract::Organ transplantation is often the only effective treatment for patients with end-stage diseases, such as heart, liver, kidney and small bowel failure and is carried out frequently worldwide. Still the post-transplantation complications remain health- and life-threatening outcome that needed to be resolved. With the r...
journal_title:American journal of translational research
pub_type: 杂志文章,评审
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更新日期:2018-11-15 00:00:00
abstract::Extracorporeal pulsed electromagnetic field (PEMF) has been shown the ability to improve regeneration in various ischemic episodes. Here, we examined whether PEMF therapy facilitate cardiac recovery in rat myocardial infarction (MI), and the cellular/molecular mechanisms underlying PEMF-related therapy was further inv...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2014-05-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-02-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2010-06-10 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章,收录出版
doi:
更新日期:2015-12-15 00:00:00
abstract:BACKGROUND:Nasopharyngeal carcinoma (NPC) is a highly invasive malignancy which has unique characteristics when found among individuals from certain ethnic or geographic populations. The role and molecular mechanism of Polo-like kinase 1 (PLK1) in NPC remain yet to be clarified. Hence, the aim of this study is to ident...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-12-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2021-01-15 00:00:00
abstract::Breast cancer, a worldwide disease with increasing incidence, develops from ductal/lobular epithelium. Nipple aspirate fluid (NAF), secreted from the breast ducts and lobules, can be analyzed to assess metabolic activity in breast microenvironment. Premalignant and malignant cell alterations may produce biochemical si...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2010-10-30 00:00:00
abstract:BACKGROUND:A meta-analysis was conducted to evaluate the accuracy of MRI, CT and FDG PET/CT in TNM stage of nasopharyngeal carcinoma patients (NPC). METHODS:Through a search of studies from 1996 to April 2015, pooled estimated sensitivity, specificity, pooled diagnostic odds ratio (DOR), summary receiver operating cha...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-11-15 00:00:00
abstract:BACKGROUND:Atherosclerosis remains a leading cause of cardiology disease worldwide, which vascular smooth muscle cells (VSMCs) proliferation and apoptosis are involved. Increasing evidences have revealed that long non-coding RNAs (lncRNAs) considered to be critical regulatory factors of VSMCs function. However, the mol...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-06-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-03-15 00:00:00
abstract::Human epithelial mucin, the major glycoprotein component of mucus, plays a critical role in host innate defense response against invading microbes by facilitating the mucociliary clearance. Excess mucin production, however, overwhelms the mucociliary clearance, resulting in not only defective mucosal defense but also ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2009-05-08 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-10-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-05-15 00:00:00
abstract::Neural Wiskott-Aldrich syndrome protein (N-WASP) is an important member of the WASP family involved in the actin cytoskeleton reorganization. Recent evidence suggests that N-WASP may play important roles in tumor progression and metastasis. However, the contribution of N-WASP to cervical cancer is still unknown. The p...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-02-15 00:00:00
abstract::The purpose of the present study was to assess the ocular surface health status in primary open angle glaucoma (POAG) patients switching from topical application of preserved latanoprost (LT) to preservative-free tafluprost (PFT) by tear proteomic monitoring. Tear fluid of POAG patients showing dry eye symptoms, using...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-10-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-03-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章,已发布勘误
doi:
更新日期:2018-12-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-05-15 00:00:00
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journal_title:American journal of translational research
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doi:
更新日期:2019-04-15 00:00:00
abstract::miR-101 is significantly downregulated in various human cancers, including oral squamous cell carcinoma (OSCC). However, the role of miR-101 in OSCC has not been elucidated. In this study, miR-101 lowly expressed in OSCC tissues and cell lines compared with that in adjacent normal tissues and human normal oral keratin...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-11-15 00:00:00
abstract::Cyclooxygenase-2 expression by malignant tumors, including colonic adenocarcinoma, is associated with increased tumor aggression and poor prognosis. Nuclear factor kappa B is a key regulator of cyclooxygenase-2 and is regulated by two pathways, the 'canonical' and the 'alternative' pathway. The alternative pathway is ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2015-09-15 00:00:00
abstract::This study aimed to determine applicable value of DWIBS in diagnosis of solitary pulmonary lesions. This study involved 32 solitary lung disease patients. T1W1, T2W1, T2WI-SPAIR were examined using MRI scanner and analyzed with View-forum 6.0 workstation. Imaging characteristics of pulmonary solitary lesions on DWIBS ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2021-01-15 00:00:00
abstract:BACKGROUND:Infection can induce and aggravate chronic kidney disease (CKD), and the chemotaxis of Th22 cells may aggravate CKD. However, the mechanism underlying group A Streptococcus (GAS) infections in CKD through the chemotaxis of Th22 cells remains unknown. METHODS:The experiment was divided into a normal control ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-12-15 00:00:00
abstract::Evidence has shown that gastrin-releasing peptide receptor (GRPR) is involved in responses to stress and anxiety. The primary role of GRPR is to stimulate corticotrophin-releasing hormone (CRH) or adrenocorticotropic hormone (ACTH) secretion. Thus, the mechanisms of GRPR signaling should be elucidated to discover nove...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-07-15 00:00:00
abstract::Glioma is an aggressive nervous system tumor with poor prognosis. Although the therapeutic strategies to overcome glioma have been improved largely recent years, the potential mechanism of its carcinogenesis remains largely unclear. The present study aimed to investigate the role of long non-coding RNA HOMEOBOX A11 an...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-10-15 00:00:00