Abstract：
:BACKGROUND This study aimed to investigate the role of miRNA-339-5p in pancreatic cancer cell invasion and migration. MATERIAL AND METHODS The differences between exosomal miRNAs of PANC02 and PANC02-H7 were studied by microarray analysis. We measured miRNA-339-5p expression in different groups; differences in cell invasion and migration were evaluated using the Transwell and wound healing assays and expression of relative proteins (E-cadherin, vimentin and ZNF689) was measured by WB assay. The correlation between miRNA-339-5p and ZNF689 expression was evaluated by luciferase reporter gene assay. RESULTS Compared with PANC02 exosome, microarray analysis indicated that miRNA-339-5p mRNA expression was significantly suppressed (P<0.001) in the PANC02-H7 exosome. Supplementation with miR-339-5p mimics led to a significant decrease in the invasion cell number and wound healing rate (P<0.001), with significantly enhanced E-cadherin expression and suppressed vimentin expression (P<0.001). However, transfection of a miR-339-5p inhibitor led to a significant increase in the invasion cell number and wound healing rate (P<0.001), with significantly suppressed E-cadherin expression and increased vimentin expression (P<0.001). Luciferase reporter gene assay demonstrated ZNF689 gene to be the target of miR-339-5p in the PANC02-H7 cell. With miR-339-5p and ZNF689 transfection, the invasion cell number and wound healing rate were significantly increased compared with those in the miR-339-5p group (P<0.001), with significantly increased expression of ZNF689 and vimentin and suppressed E-cadherin expression (P<0.001). CONCLUSIONS miR-339-5p suppresses the invasion and migration of pancreatic cancer cells via direct regulation of ZNF689 in vitro.
journal_name
Med Sci Monitjournal_title
Medical science monitor : international medical journal of experimental and clinical researchauthors
Yu Z,Zhao S,Wang L,Wang J,Zhou Jdoi
10.12659/MSM.917038subject
Has Abstractpub_date
2019-10-07 00:00:00pages
7509-7517eissn
1234-1010issn
1643-3750pii
917038journal_volume
25pub_type
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.917013
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
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doi：
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.890920
更新日期：2014-12-06 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.916855
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.894086
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2002-09-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2008-05-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.895689
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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更新日期：2018-06-15 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2005-06-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2002-10-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2001-05-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2004-06-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.914930
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
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doi：
更新日期：2000-03-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
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doi：
更新日期：2007-09-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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更新日期：2012-05-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
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更新日期：2018-11-03 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2005-12-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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更新日期：2019-12-19 00:00:00
